Mackload said:

Qq why isn't spores bukkake a thing on this site?

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DiscoDave said:

@Kloakk I guess it makes sense we have the same experience, as I am working with your genetics

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Should it matter when you start from spores? I got my spores from Nektar, and the print you got was from a first flush of that, but

Phaethon said:

If you fold the bags carefully, and let the pressure cooker cool before removing the bags, they will basically seal themselves. I just carefully take out the bags and put them into a tote. I wipe the outside of the sealed lip with isopropyl before opening it up (in front of an FFU) to inoculate.

How are you planning to inoculate substrate bags in a SAB?

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Quote:

You seen this?

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So awesome

Phaethon said:

You can preseal the bags, but there's a higher risk of the bags failing as the air inside expands and can't get out fast enough during heating. The seal from the contraction of steam during cooling is pretty effective - I can't remember one contaminating due to that seal failing, and I never unload the cooker under sterile conditions.

Hope you have a very large SAB.

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tultedspoon said:

Is all tap water generally ok for misting? If it's heavy in calcium could that be problematic?

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markh said:

I have 4 jars of oats that I injected Azure LC into over a month ago and no signs of life yet. Assuming the LC was dead. I have 1 jar of ape spawn that has been consolidating for 6 weeks now. My question is, can I use the 4 jars that did not take and do G2G with the Ape spawn into those jars? Or should I just make more jars of grain?

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FounderZ said:

I sealed the bags, we'll see if they blow up... lmfao I wasn't able to vacuum seal them (machine failed) so we'll see how bloated they are after 90 minutes at 15PSI. If no explosions we'll try this a few times till failure or never stop. I'll update with photo's if there was a blow out! What the damage/mess is.

First round, no seal PC @ 15PSI for 2hrs, transfer to sealer (open air kitchen) immediately and put in still air box to chill out. Second round seal and PC @ 15PSI for 90 minutes.

Hoping second rounds take so I can fearlessly run bags.

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Phaethon said:

FounderZ said:

I sealed the bags, we'll see if they blow up... lmfao I wasn't able to vacuum seal them (machine failed) so we'll see how bloated they are after 90 minutes at 15PSI. If no explosions we'll try this a few times till failure or never stop. I'll update with photo's if there was a blow out! What the damage/mess is.

First round, no seal PC @ 15PSI for 2hrs, transfer to sealer (open air kitchen) immediately and put in still air box to chill out. Second round seal and PC @ 15PSI for 90 minutes.

Hoping second rounds take so I can fearlessly run bags.

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People seal them up first without vacuum. You won't see them bloated - by the time you open the PC they will have cooled. My concern is while they are heating, the air inside will expand and the filter heavily restricts the expanded gas from escaping. I don't think the risk of failure is super high with the filter type on a typical grow bag (14A), but you really can just not seal it until you inoculate it, and save yourself a seal and cut cycle. The contracting bag seals against itself until you physically pull apart the bag opening.

How big are these bags and what are you putting in them? I don't go less than 3.5 hours for 5 lb bags of supplemented hardwood pellets, but that's with 4 or 8 fairly tightly packed in the PC or sterilizer. (The only sterilized substrate I've used for cubes has been in quart containers, which I only do for a couple hours.)

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Vacuum wouldn't work, bag type wasn't right. So I did just that sealed it and PC'd it. I threw just two bags in 5LB mixes each one  of hardwood and wheat bran. Water levels good nice field capacity.

There was some air in on of the bags before PC I think the vacuum + seal was a flop but I am testing it's just $1.00 in materials and a waste of time but we'll experiment and get comfortable.

I'll know in about 20 minutes when I open that hot lid what's up. I'll probably lay them so air can escape easy while sitting in the still air box.

You're saying the cooling process inside the cooker seals those bags tighter than ever? The bags are relatively big, I got the
60-Pack Autoclavable Mushroom Growing Bag Bulk with Microporous Filter Patchs - Large 8"x5"x20" Extra Thick 80 Micron (=3 Mil)| Mushroom Cultivation Spawn Supply Myco Bag Bulk Substrate Bags

Also for PC time, I've always been a 60 minute guy never issues, I'm being generous when I did 2 hours the first time, I'll probably do tests with 60 minutes more and bench them to see while I stick to a 1hr and 30 minute basis atm, this may change but I'm lazy, though sterile, lazy.

*edit*
I'll take pictures when that lid opens, we've either got a nice mess on our hands or don't. I do think my dogs heard something at the 30 minute mark of pressure cooking so we'll see soon if the pot had something explode. (I don't monitor my pressure cookers no more I put to many safeties in place).

Phaethon said:

Pretty sure that's the size I have. Unicorn calls them 14A.

This one has been sitting in a tote for months:


(The culture I tried with a few from this batch didn't like the beet pulp pellets I supplemented these with...and then I fell out of the hobby for a while. I'll probably still try to use these for oysters though.)

It's still perfectly sealed up, just from folding the bag before sterilizing and letting it cool in the sterilizer (some in an AA 75X and some in a Presto 23 quart canner.)

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