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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Kunta Kente]
    #29475690 -

How long can I store steam-sterilized pf-tek BRF jars at room temperature before needing to either refrigerate or inoculate? I think because BRF PF-tek jars sit after inoculation for weeks, that I can wait like 1 day after sterilization and removal from steaming pot, before inoculation? Thanks =)

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Re: Ask Quick Questions, Get Quick Answers [Re: HydroShroom24]
    #29475712 -


You should be able to wait a couple of weeks. Most likely it'll dry out and start pulling away from the sides before contaminating.

See Mr Piggy's post below.


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LAGM 2026 grow log | My library/trade list

Edited by Phaethon (02/06/26 07:17 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: HydroShroom24] * 1
    #29475715 -

I would use them when you make them (and they have cooled) if you are steam sanitizing (it’s not sterilizing) and are using a traditional dry verm barrier.  Day or two ideal range probably.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29475725 -

I defer. :smile: I wrote that comment with my experience with pressure cooked BRF pucks in mind.


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 1
    #29475762 -



I picked this little fella off of one of my cakes because he was looking a little brown.

I believe he was on my cake while it was in the jar, there are a few smaller/larger guys that also have some slight browning. I think it's just bruising from the dunk and roll, but I figure I'd ask to be safe. I have lots of new pins that are perfectly white, so if the brownish ones are rotting, I'd rather pluck em then risk it.

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29476087 -

Phaethon said:
I defer. :smile: I wrote that comment with my experience with pressure cooked BRF pucks in mind.


I’m also totally guessing so yours is as good as mine :shrug:


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29476113 -

Are cyrovials like these sterile from the factory?

I’m trading some wedges and would hate to do so in a non-sterile vial. I haven’t opened these or used them previously.


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Re: Ask Quick Questions, Get Quick Answers [Re: bongoman]
    #29476136 -

I don't believe they are. If they where it would definitely make a point to say so in the description where you bought them.

Better safe than sorry though. just a quick run 15 min run Will do.

Plus people usually put water in them. so you'd have to sterilize that anyways.

( water is optional but I would imagine its preferred. I've never done it without water though so I couldn't actually tell you)

Also be advised if you're planning on sending in regular mail it won't work because they can't make it through the machines.  unless you pay extra postage for non-machinable.

otherwise you'll have to send an actual package..

Make sure you ask the person you're sending to if that's okay with them.


Ps. Small Ziploc Gram baggies work perfectly for sending regular letters with wedges through the mail.




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:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: bongoman] * 1
    #29476177 -

:whathesaid:

Might be sterile, but why take the chance. You can fit 14 of them in a 4 oz jar. I often use the Instant Pot instead of setting up the 23 quart pressure cooker for these. 30-45 minute cycle just to be sure since it's lower PSI.

For sending wedges on the cheap, check out Beef's smash wedge writeup. If you don't mind spending a bit more to mail a padded envelope (about $6 for domestic US mail), you can definitely use the 2ml cryos to put wedges in, with or without sterile water. But most people when mailing fresh wedges use small eppendorf centrifuge tubes (or baggies as in Beef's tek).

Edit: Sorry, didn't mean to discourage you from using what you already have to trade cultures. Eppendorf tubes are cheaper but even cheaper is what you've already got!


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LAGM 2026 grow log | My library/trade list

Edited by Phaethon (02/07/26 01:19 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29476209 -

:specialralph:

Hi, I have a quick question.

On the one hand, people press coir mixed with grains using a road roller, while on the other hand people grow mushrooms on a substrate suspended loosely in levitation chambers.

How does this relate to the ratio?
If I have a glass with tightly compacted coir vs. loosely coir, both will have the same volume but different weights. Which coir — compacted or loose, should be included in the ratio?


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.
"How do you eat an elephant? One bite at a time."

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 1
    #29476211 -

I like the "pill pouch" baggies from the pharmacy. They're a good size and they're thick so they're easy to handle And bend to hold open when putting wedges inside of them. Couple bucks for a hundred I think.



These mylar bags


are good too. But I have had some issues with one or two here and there leaking out the top when they get smashed.

  However the receivers said it never was a problem. And I would just make sure to tape them in the future or double bag them.both seems to work.


I also like using the little eppi tubes.


And if you do choose to go to padded envelope route or whatever make sure you go to a place like pirateship.com and buy your own label.  Its cheaper.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


Edited by callmehippie (02/07/26 12:20 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Blizzloo]
    #29476305 -

Blizzloo said:
:specialralph:

Hi, I have a quick question.

On the one hand, people press coir mixed with grains using a road roller, while on the other hand people grow mushrooms on a substrate suspended loosely in levitation chambers.

How does this relate to the ratio?
If I have a glass with tightly compacted coir vs. loosely coir, both will have the same volume but different weights. Which coir — compacted or loose, should be included in the ratio?


Ratio of what? Grain to substrate? My ratio is determined by how clean and healthy my grain is. Suspect grain gets a lower ratio like 1:1, good clean healthy grain I will go up to 1:4 grain to coir sometimes.

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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog]
    #29476309 -

levitation chamber?


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog]
    #29476356 -

Arkyhog said:
Ratio of what? Grain to substrate? My ratio is determined by how clean and healthy my grain is. Suspect grain gets a lower ratio like 1:1, good clean healthy grain I will go up to 1:4 grain to coir sometimes.


Thanks for your reply.
I am new to coir and so far, nowhere I have read has anyone mentioned this approach, but it sounds reasonable, thx.

As for my question, generally, everywhere I look, I only see people experimenting with ratios ranging from 1:1 to even 1:6. I'm just wondering about this - grains (of anything, for simplicity's sake) with a volume of 1 cup cannot really be packed any more tightly into such a volume. Coir, on the other hand, is a different matter. You can pour coir very loosely into a 1 cup container, then tap the container to make room for more, then you can even push it down with a spoon and add more, and finally, you can even use all your strength and compress it to the max, which will ultimately turn the initial 1 cup loosely poured in into 1/5-1/4 cup.

As I don't know much about this method yet, I decided to ask someone knowledgeable how it should be done.

Coir loose or compressed should be taken into account when calculating the volume before mixing with the grains?


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.
"How do you eat an elephant? One bite at a time."

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Re: Ask Quick Questions, Get Quick Answers [Re: Blizzloo]
    #29476382 -

Blizzloo said:
Arkyhog said:
Ratio of what? Grain to substrate? My ratio is determined by how clean and healthy my grain is. Suspect grain gets a lower ratio like 1:1, good clean healthy grain I will go up to 1:4 grain to coir sometimes.


Thanks for your reply.
I am new to coir and so far, nowhere I have read has anyone mentioned this approach, but it sounds reasonable, thx.

As for my question, generally, everywhere I look, I only see people experimenting with ratios ranging from 1:1 to even 1:6. I'm just wondering about this - grains (of anything, for simplicity's sake) with a volume of 1 cup cannot really be packed any more tightly into such a volume. Coir, on the other hand, is a different matter. You can pour coir very loosely into a 1 cup container, then tap the container to make room for more, then you can even push it down with a spoon and add more, and finally, you can even use all your strength and compress it to the max, which will ultimately turn the initial 1 cup loosely poured in into 1/5-1/4 cup.

As I don't know much about this method yet, I decided to ask someone knowledgeable how it should be done.

Coir loose or compressed should be taken into account when calculating the volume before mixing with the grains?


Ah I got you now. Once i decide my mix ratio based of the health of the grain spawn, I mix it in a large zip lock bag or 20qt tub with good matching lid so I can mix the hell out of it with the coir. Then I just fill up what I want to grow in to the height required. Usually 1.5 inches to 2 inches deep in my 20qt tubs to keep it right below the moded holes or completely full on small trays, or 1 inch to 2 in a shoe box. Once you grow for awhile your going to have all kinds of containers you grow mushrooms in. I always press my substrate by the way once in the tub or trays, but I do a loose casing on second flushes.

Edit: Had to correct spelling and clarify instructions for compacting. Thanks Piggy for having my back, I need to start reading through my post before submitting!

Edited by Arkyhog (02/07/26 12:08 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog] * 2
    #29476413 -

Loose.  You compact it after setting up a tub, not before.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29476454 -

So I was out of town while I had quite a few jars colonizing. Unfortunately, temps were relatively low, closer to 18 Celcius inside the house.

A lot of the jars are fully colonized, but appear wispy. These have been shaken when I got back and left to recover, but I guess the colder temperatures might've made them lazier/whispier? Do I need to let them consolidate?



I was using some old plates as well, trying to get prints from genetics I would've otherwise lost.

A few of my jars might've been under hydrated, which is another thing that could've caused this.

Anyhow, these are going to be for shoeboxes, not g2g or big bulk. I just want to re-acquire my collection cause I was a fool and threw out my bag of prints while cleaning


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I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29476455 -

If you put a cup of Coir in first and then pack it down it's still a cup of coir it doesn't matter if you packed it.

Just because it's packed down and now only measures to maby 1/4cup. In the container  The volume of is still the same.

100 lb of stones doesn't weigh more than 100 lb of feathers even if  the feathers can be packed in more.

It dosnt change.  I mean sure if you add more then it changes..


Stick to arkys and piggys advice it's golden.



--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29476488 -

Spore swabs sort of escape me and Im never too sure if I hit contam or not.





Its easier to tell with spore prints, but did these cultivate any mycelium?


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Successful Grows: ONE

Agar Plates Gone Through: 46
Agar Plates successfully cultivated: 6

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Re: Ask Quick Questions, Get Quick Answers [Re: ladynightshade]
    #29476493 -

I think so, put it on a new plate and see if it grows well


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Extras: Filter Print Post Top
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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