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andymc
cocoa beetles from zanzibar

Registered: 11/25/09
Posts: 2,635

Re: Ask Quick Questions, Get Quick Answers

[Re: callmehippie]

4
#29453274 - 01/12/26 04:37 AM (9 months, 25 days ago)

callmehippie said:

Is it okay if I leave it hanging in this Brew bag outside like this and come back to it tomorrow?

Around here, the birds would be into that in no time. You just have to wake up earlier than the ... ok it's too corny, I can't type the rest :birdfeed:

:birdfeed:

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How I make spore prints
FFU build

"The idiots ~~are taking~~ have taken over" -NOFX

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Mackload
Bungler

Registered: 11/14/23
Posts: 4,259
Loc: No0b purgatory
Last seen: 50 minutes, 11 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: AwareWithoutSelf]

1
#29453309 - 01/12/26 07:30 AM (9 months, 25 days ago)

The important thing is good surface conditions with maximum air, if you can get that with the sponges, great. And if your substrate is at field capacity you might not have to spray at all. However you set it up so you get tiny beads of moisture across the mycelium once it appears, without misting, is the right way to do it.

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Learn it, know it, live it

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CaptainTrips420

Registered: 09/28/21
Posts: 2,891
Last seen: 1 month, 7 days

Re: Ask Quick Questions, Get Quick Answers

1
#29453335 - 01/12/26 08:17 AM (9 months, 25 days ago)

Is primordia in cubensis white at first? This morning my casing has a bunch of tiny dots that look like bumps but all white. Im not sure if it is primordia pinning but its a different looking growth its to small to take a picture properly plus im trying to keep it as humid as possible ill know for sure in a few days but I suspect its pins.

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I am the master of my fate,
I am the captain of my soul.

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BagShaker
Noob, but enthusiast

Registered: 12/19/25
Posts: 83
Last seen: 5 months, 4 days

Re: Ask Quick Questions, Get Quick Answers

[Re: BagShaker]
#29453367 - 01/12/26 09:09 AM (9 months, 25 days ago)

What kind of bacteria /toxin in this? thank you

Edited by BagShaker (01/12/26 09:10 AM)

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andymc
cocoa beetles from zanzibar

Registered: 11/25/09
Posts: 2,635

Re: Ask Quick Questions, Get Quick Answers

[Re: CaptainTrips420]
#29453403 - 01/12/26 10:23 AM (9 months, 25 days ago)

CaptainTrips420 said:

Is primordia in cubensis white at first?

Yes

:thumbup:

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How I make spore prints
FFU build

"The idiots ~~are taking~~ have taken over" -NOFX

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The Tao
Read more, post less.

Registered: 09/12/19
Posts: 3,936
Loc: Canada

Last seen: 47 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: CaptainTrips420]
#29453412 - 01/12/26 10:51 AM (9 months, 25 days ago)

CaptainTrips420 said:

Is primordia in cubensis white at first? This morning my casing has a bunch of tiny dots that look like bumps but all white. Im not sure if it is primordia pinning but its a different looking growth its to small to take a picture properly plus im trying to keep it as humid as possible ill know for sure in a few days but I suspect its pins.

Does it look like this?

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,584
Loc: Exact Center
Last seen: 1 minute, 49 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: BagShaker]
#29453413 - 01/12/26 10:59 AM (9 months, 25 days ago)

Yes cap, prinordia are white
Bag Shaker, it looks genetic not environmental (contam)

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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jeffreykite
Stranger

Registered: 12/30/25
Posts: 72
Last seen: 1 month, 14 days

Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]
#29453486 - 01/12/26 01:01 PM (9 months, 25 days ago)

Should I tape the inoculation points on my pf tek jars, or just leave em? I inoculated yesterday.

I did do the dry verm layer at the top of my jars, but I just want to be sure before I leave them alone for a while.

Edited by jeffreykite (01/12/26 01:06 PM)

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flapping_bird
Human, Be-ing

Registered: 01/03/25
Posts: 1,848

Re: Ask Quick Questions, Get Quick Answers

[Re: jeffreykite]
#29453562 - 01/12/26 02:34 PM (9 months, 25 days ago)

jeffreykite said:

Should I tape the inoculation points on my pf tek jars, or just leave em? I inoculated yesterday.

I did do the dry verm layer at the top of my jars, but I just want to be sure before I leave them alone for a while.

You don’t necessarily need to, the dry verm layer is intended to seve the same function as tape would, but, me being a “belt and suspenders “ kinda guy, if you have tape, I feel there is no harm in using it & only good can come from doing so. Best of luck to you on your grow!

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CaptainTrips420

Registered: 09/28/21
Posts: 2,891
Last seen: 1 month, 7 days

Re: Ask Quick Questions, Get Quick Answers

[Re: The Tao]
#29453580 - 01/12/26 02:58 PM (9 months, 25 days ago)

The Tao said:

CaptainTrips420 said:

Is primordia in cubensis white at first? This morning my casing has a bunch of tiny dots that look like bumps but all white. Im not sure if it is primordia pinning but its a different looking growth its to small to take a picture properly plus im trying to keep it as humid as possible ill know for sure in a few days but I suspect its pins.

Does it look like this?

Cool ya the little white spots but mine has no pins I never noticed them before because only seen pictures they seem to be getting larger even in the regular looking mycelium.

mine are really small just little dots that I noticed this morning it had been going rhizomorphic from coirs low nutrient.

The liquid culture replacement my vendor sent haves very fast mycelium but its got little black spots in it. When I used it I did maybe .5 CC on agar it hasn't shown contamination but is looking healthy and completely contamination free so far. I am afraid to use it because it has little black spots I don't understand how they didn't get on the agar its a low amount in the syringe though I missed it at first didn't notice till recently.

I don't think I should use it further and even transfer the agar before using just to be safe im really surprised the last batch was so much worse.

--------------------

I am the master of my fate,
I am the captain of my soul.

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shroomerhumor
Professional Noob

Registered: 08/12/21
Posts: 155
Last seen: 6 months, 17 days

Re: Ask Quick Questions, Get Quick Answers

[Re: CaptainTrips420]
#29453602 - 01/12/26 03:38 PM (9 months, 25 days ago)

My LC syringe from vendor also had little black spots. They explained that it was from the rust of the metal lid where the ports were drilled.. so far no problem on my side.

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shroomerhumor
Professional Noob

Registered: 08/12/21
Posts: 155
Last seen: 6 months, 17 days

Re: Ask Quick Questions, Get Quick Answers

[Re: shroomerhumor]
#29453606 - 01/12/26 03:46 PM (9 months, 25 days ago)

This was a few days after 1-5-26 incubating around 72f. Are the little spots bacterial contam? All 25 plates had such spots.

They're not visible anymore. Here's another plate from today (yes I should've numbered them)

Thanks for the help guys :smile:

:smile:

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Kloakk
Strange

Registered: 10/21/24
Posts: 6,437
Loc: Scandinavia
Last seen: 4 hours, 23 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: shroomerhumor]

1
#29453611 - 01/12/26 03:51 PM (9 months, 25 days ago)

shroomerhumor said:

Are the little spots bacterial contam?

Yes, best would be to take a new transfers before mycelium reaches the bacteria. If you take transfers now I would take them through a cabin.

--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett

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shroomerhumor
Professional Noob

Registered: 08/12/21
Posts: 155
Last seen: 6 months, 17 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Kloakk]
#29453625 - 01/12/26 04:11 PM (9 months, 25 days ago)

Kloakk said:

shroomerhumor said:

Are the little spots bacterial contam?

Yes, best would be to take a new transfers before mycelium reaches the bacteria. If you take transfers now I would take them through a cabin.

Dude that's an awesome tek. Thank you! I had made 5 A2A transfers early on using the regular method and chose the cleanest sectors.
I posted a picture of that plate in my journal and it seems clean so far. My only worry about doing the cabin method in my SAB is the more prolonged air exposure time.

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JuniperTea
Stranger

Registered: 11/14/25
Posts: 89
Last seen: 2 months, 7 days

Re: Ask Quick Questions, Get Quick Answers

[Re: shroomerhumor]

2
#29453643 - 01/12/26 04:29 PM (9 months, 25 days ago)

Any body got experience with hillbilly pumpkin? This dudes are hilarious, how tall could I expect them to get. Im having trouble finding examples of what they look like when their veil breaks. There about 173993 tiny pins going, I'm so curious as to how it will turn out

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Binary thinking is the best thinking.

plz, tell me exactly what to do :wink:

:wink:

.

If more of us valued food and cheer and song above hoarded gold, it would be a merrier world.

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xsanriu
Stranger

Registered: 09/24/24
Posts: 67
Last seen: 1 month, 25 days

Re: Ask Quick Questions, Get Quick Answers

[Re: JuniperTea]
#29453647 - 01/12/26 04:31 PM (9 months, 25 days ago)

Two bins from earlier have made no progress in a week except one little side pin. I have jars ready to go. Should I dump the bins and do a bleach blast at this point?

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Nokumana
Trying to walk a path

Registered: 12/11/25
Posts: 762
Loc: No matter where I go, there I ...
Last seen: 6 days, 11 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: shroomerhumor]

3
#29453657 - 01/12/26 04:43 PM (9 months, 25 days ago)

shroomerhumor said:

Kloakk said:

shroomerhumor said:

Are the little spots bacterial contam?

Yes, best would be to take a new transfers before mycelium reaches the bacteria. If you take transfers now I would take them through a cabin.

Dude that's an awesome tek. Thank you! I had made 5 A2A transfers early on using the regular method and chose the cleanest sectors.
I posted a picture of that plate in my journal and it seems clean so far. My only worry about doing the cabin method in my SAB is the more prolonged air exposure time.

Speaking as a noob who had similar worries, I can tell you it's not as bad as it seems. I just did five cabin sequestrations over the weekend to try and salvage a culture.

Once you get everything set up in your box and you have thought through the plan, things go pretty smoothly. Keep your plate that you're going to take the roofs from upside down. Pick it up and cut a checkerboard into it so you can just go in and pluck out a roof when you are ready. Then set yourself up to take the wedge from your contaminated plate and put it into a plate into which you've already cut out the cabin. Once you've got the wedge in place, putting the roof on top is pretty elementary. One thing that took a bit of learning for me was making sure I could get the roof off of the scalpel blade easily. A second instrument like a sterile pair of pickups might be helpful but would also increase your open air time

--------------------
-Noku
Eskawata kaya waikiki
HHG
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CaptainTrips420

Registered: 09/28/21
Posts: 2,891
Last seen: 1 month, 7 days

Re: Ask Quick Questions, Get Quick Answers

[Re: shroomerhumor]
#29453659 - 01/12/26 04:44 PM (9 months, 25 days ago)

shroomerhumor said:

My LC syringe from vendor also had little black spots. They explained that it was from the rust of the metal lid where the ports were drilled.. so far no problem on my side.

Dang sorry about the bacteria. Mine doesn't have any contamination so I figured its just the rust you were talking about I did a bunch of jars hopefully it will turn out alright. I have been thinking about making my own LC but I have never had success with LC ever before.

--------------------

I am the master of my fate,
I am the captain of my soul.

Extras:

shroomerhumor
Professional Noob

Registered: 08/12/21
Posts: 155
Last seen: 6 months, 17 days

Re: Ask Quick Questions, Get Quick Answers

[Re: CaptainTrips420]
#29453722 - 01/12/26 06:26 PM (9 months, 25 days ago)

xsanriu said:

Two bins from earlier have made no progress in a week except one little side pin. I have jars ready to go. Should I dump the bins and do a bleach blast at this point?

Show clear pictures of the whole substrate surfaces. What date is "earlier" and what date did you make the tubs?

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shroomerhumor
Professional Noob

Registered: 08/12/21
Posts: 155
Last seen: 6 months, 17 days

Re: Ask Quick Questions, Get Quick Answers

[Re: CaptainTrips420]
#29453726 - 01/12/26 06:31 PM (9 months, 25 days ago)

CaptainTrips420 said:

shroomerhumor said:

My LC syringe from vendor also had little black spots. They explained that it was from the rust of the metal lid where the ports were drilled.. so far no problem on my side.

Dang sorry about the bacteria. Mine doesn't have any contamination so I figured its just the rust you were talking about I did a bunch of jars hopefully it will turn out alright. I have been thinking about making my own LC but I have never had success with LC ever before.

If you want a peace of mind then just message the vendor to confirm. What are you growing now? I am trying to get golden teacher going. How come you've never had success making an LC? It looks very easy to do and it's something I will attempt sooner than later.

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