Huskies said:

AstroSporeFiend said:

Senescence question. If a variety doesn’t drop many spores, therefore pretty much needs to be cloned on agar, grown out, cloned again, grown out, etc… is senescence avoided by the fruiting process? Just something I was thinking about earlier.

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Consider Enigma, which is sporeless, and must be cloned.

Thousands of people have been cultivating it for years through that, still seems to be good.

I think the fears of senescence are more due to industrial growing of commercial strains.

That being said, going back to spore more often can be a good idea, I think. Even varieties that don't drop spores can be swabbed with sterile q-tips (I wrap 6 in aluminum foil and PC them, then place them in baggies)

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Phaethon said:

I suspect if our actual process was as you described (fruit, clone from fruit, grow out that clone, repeat ad nauseum), senescence might actually be something to worry about. It's more about cell division than time, and that's a whole lot of cell divisions from the first spore to the (say) 20th serially cloned mushroom. (And that is how senescence rears its head in the commercial button mushroom business too - the big spawn producers expand a tiny amount of "young" culture into thousands of pounds of spawn via many grain to grain generations.)

What we actually do is clone a mushroom, and keep the resulting culture around for later grows. Even if you don't use some kind of stasis storage, the number of cell divisions leading from that first clone plate to your (say) T20 plate is essentially negligible compared to 20 serial g2g expansions or 20 serial clone-grow-clone... cycles.

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Mackload said:

xsanriu said:

Tubs in a tent

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Nichrome said:

Fuck no. Leucoagaricus gongylophorus has been tended as a clonal culture by the ants and has not produced spores in millions of years.

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Mr Piggy said:

Nichrome said:

Fuck no. Leucoagaricus gongylophorus has been tended as a clonal culture by the ants and has not produced spores in millions of years.

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Cannot imagine how horny that myc is.

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