AZ Starman2112 said:

By strong stuff I just meant that you need a lot less of the yeast than you do LME to make a nutrient boost in the agar. 1g is like nothing compared to 16g.

I am going to make a .5 batch and a normal batch with the added yeast. I’ll experiment. I’ve got nothing to lose but plates and time.

Your suggestion of transferring to both type of plates is a good one.

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I see, well the DME(dry malt extract) is mainly simple sugars(food), the nitrogen is used  mainly for digesting that food and build new biomass(on agar), so even though you are adding more nutrients, they are for different things. Like you can't fix a plant with nitrogen deficiency by giving it phosphorus, if that makes sense.

I'll do it with you, I have some T0 pan cyan plates I'm going to transfer to T1's tomorrow, I'll take a transfer to one of my fermaid O plates as well and we can see how that develops

Kloakk said:

I see, well the DME(dry malt extract) is mainly simple sugars(food), the nitrogen is used  mainly for digesting that food and build new biomass(on agar), so even though you are adding more nutrients, they are for different things. Like you can't fix a plant with nitrogen deficiency by giving it phosphorus, if that makes sense.

I'll do it with you, I have some T0 pan cyan plates I'm going to transfer to T1's tomorrow, I'll take a transfer to one of my fermaid O plates as well and we can see how that develops

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You’ll be ahead of me by a few days, but this will be fun and informative.

Asura said:

I love seeing what you guys are doing, but ya'll do know this isn't
the official pan thread, right?

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Yeah I know, it's cozy in here tho right? But seriously, if you prefer I'll move future post about this yeast business to the official thread, just say the word and it's done, this thread is your baby

PuffM4gicDr4gon said:

If there is no official way to cultivate pans, how can there be an official thread? /philosophy

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Asura said:

I'm good with whatever, just wasn't sure if ya'll took a wrong turn

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All the time, but I often end up in interesting places.

Asura said:

I'm good with whatever, just wasn't sure if ya'll took a wrong turn

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Asura said:

Puduwoke said:

Edit: Does bisporus grow noticeably slower on agar than pan cyans? Mine seems to so thought I would ask

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I am really only now growing bisporus "regularly". Tree frog would know better, but my casual observation is
that the cyans #'s are doubled. Where something takes 5 days with cyans w/ bisporus it takes 10.

You could reach out to kirkeng too for question about bispo because that guy knows his shit.
He got me going with my latest rounds of them.

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A lot of cool stuff happening in this thread

Bisporus runs about as fast as cyans for me in all phases aside from pinning speed.  On plates, in grains, and on substrate.  Time to pin, double sounds about right.  But they mature about the same.

That said, it's been cool and bisporus are subtropical and cyans tropical.  So, they have different temperature preferences and I imagine my cyans would be much zippier if it was about 5f warmer in my lab and tent.

re: Yeast.  I've played with yeast a bit but never found it improved things on plates.  I can see adding it to jars though depending on the grain type used.  There was a study done on bisporus and the preferred carbon to nitrogin ratio was around 30:1.  Low nutrient plates likely fall within this range simply from the nitrogin in the agar agar (it's 6% protien, so contains some nitrogin already).  But maybe adding nitrogin to 2% plates would be of benefit.  And may even create better expression in the mycelium.  Also, take the study with a grain of salt in regards to it being bisporus rather than a two spored cyan strain.  Pan taxonomy is messy without sequencing.  And as far as I'm aware, the Asian Pan bisporus are all Pan cyan var. bisporus and sequence closer to ttbvi than say the san diego and colville collections we refer to here as bisporus.

AZ Starman2112 said:

Kloakk - I poured 41 0.5% plates this morning. I’m getting better at it! Had to pour real hot though because I had to leave for work. I poured with gloves on with folded up paper towels inside the gloves as soon on as the PC was cooled enough that the pressure was zero. I also used a paper towel sprayed with ISO around the neck of the bottles. Didn’t burn myself, but it might take a couple extra days for the condensation to evaporate. Gonna pour some regular plates with the yeast this week too.

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Very nice man, glad you didn't burn yourself, need those hands for cult you know

If you stacked the plates 10 or more I'm sure the condensation will have cleared except for the top two of each stack in 2-3 days(SAB), and if you have a hood or SAB with armhole covers they can stay unwrapped until they're good, well at least in a SAB, don't have a hood so I don't know if it could dry out plates quick.

Yeah do that, as I said I don't know how often I'll use yeast in plates, but I am going to do more test and see if it has a place in my cultivation. I did 2 transfers of pan cyan today, one to my regular 1,6 and one to my 1,6 with fermaid O, but it's going to be a couple of days before there is any update worth pictures.

I also got some MiB RS and Pan Jambo spores to agar, I'm excited about that as well

AZ Starman2112 said:

To some of the Pan experts, how are these jars looking? Spawned 11 days ago. First two jars with red lids are TTBVI, single jar with red lid is Pan Cyan Peace River.

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Red left looks good, red right I suggest a break & shake check, green look a little sus, nothing to worrisome, but pans really don't like bacteria and just a little can fuck them over.. Would I spawn them all? Hell yeah, Am I fine with loss? Yes I am, I just make more substrate and go again

Kloakk said:

AZ Starman2112 said:

To some of the Pan experts, how are these jars looking? Spawned 11 days ago. First two jars with red lids are TTBVI, single jar with red lid is Pan Cyan Peace River.

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Red left looks good, red right I suggest a break & shake check, green look a little sus, nothing to worrisome, but pans really don't like bacteria and just a little can fuck them over.. Would I spawn them all? Hell yeah, Am I fine with loss? Yes I am, I just make more substrate and go again

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AZ Starman2112 said:

Kloakk said:

AZ Starman2112 said:

To some of the Pan experts, how are these jars looking? Spawned 11 days ago. First two jars with red lids are TTBVI, single jar with red lid is Pan Cyan Peace River.

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Red left looks good, red right I suggest a break & shake check, green look a little sus, nothing to worrisome, but pans really don't like bacteria and just a little can fuck them over.. Would I spawn them all? Hell yeah, Am I fine with loss? Yes I am, I just make more substrate and go again

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I agree! I have another grain jar and a plate of PCPR ready to go to make a drop. I was thinking of breaking all 3 and seeing how they recover. Then make some sub when recovered and let the nose be the judge.

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