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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29418685 -

tree frog said:
Some lines of bvi are starting to show inbreeding depression.  Both are fast, fruit well, and are plenty strong.

I can't speak for the alkaloid profile, because I only grew MiB be once and I didn't eat it.  But I haven't heard anyone complain about it.



Thank you so much! For better or worse, those are the two varieties available to me. I have read that TTBVI can be a bit flinty, experience wise, but these things are so subjective.

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Re: Official Copelandia/Panaeolus Thread [Re: crossfields]
    #29418725 -

It can be strong and is often described as aggressive (both as far as fruiting and tripping).

But genetics are just one factor in both potency and alkaloid profile.  And even the genetics can vary in an individual variety.  Some BVI is just average potency, and some is very strong.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29418808 -

tree frog said:
And since no one else answered this or had an idea on what it is, I'm just going to say I've never seen anything like it before and I imagine I would compost it based on your description.

But it does look like there is a pin coming up on the side, so if you want you could let that mature and get a print.  And that might give you some idea if the rest of the tray is worth trying to save.



Thank you, tree frog.
I have tossed those cultures with that yellow stuff on it.
Had made some prints before they turned yellow, 6 dark prints and 4 weak prints, so the genetics are saved.
Have three more trays which have not turned yellow yet and made some more grain jars yesterday. Next try! :mushroom2:

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29418811 -

Anomalocaris said:
That dont look good my dude. Some contamination.

Once again the gods ram cock in fuckin ass



Yeah, an elephant cock.
But one day i will fuck back. :grin:

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Re: Official Copelandia/Panaeolus Thread [Re: psychedelicmind]
    #29419402 -

Just curious if the running consensus with cyans is (still?) either T0 or T1 to grain. That is T0 is spore print to agar > if clean take edges to grain. Obviously T1 is one transfer to agar beyond that.


Also... I have some really old (like, a year?) condiment cup T0 that I had done. The agar is highly likely to be dried by now but I haven't checked. Would you guys bother using them (the presumably clean growth) and if so how?

I'll post pics of a few in an hour or so, if helpful


--------------------
My posts are long winded and dense.
Supplemented coir experiments< Currently ongoing spring 2026.

~Agar~ 1000mL water = 1000g water. use ~1% agar...1% of 1000g is 10g.  For cubes, use between 1-2% nutes (10-20g). For pans use 0.5% nutes (5g).

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.

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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente] * 1
    #29419421 -

Ime going many transfers in, like 7+ leads to weaker flushes. But going with T0 and T1 is a crap shoot. Maybe I get a vigorous flusher and maybe I get nothing or contams from what looked like a clean plate. Maybe my eye for various pan myc is not fully dialed in, but I get the most reliable results from a clean T2 to T4.

I do okay with clones and stored LI as well, which some others avoid. So ymmv.

I don't have experience with growing out from an old agar plate for pans, but I wouldn't hesitate to do a transfer and see what happens.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Official Copelandia/Panaeolus Thread [Re: Kunta Kente] * 3
    #29419425 -

I think the t0/t1 thing was a fad.  Faht has been doing excellent work with clones.  And if early was really that much better, cloned pans would be horrible.

Clean growth > send it early.

Likewise, if your plate culture revives after transferring to a clean plate, I don't see any issues with running it.

Beaten so :whathesaid:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (12/04/25 12:45 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 4
    #29419458 -

tree frog said:
I think the t0/t1 thing was a fad.



Possibly. I've only ever done it because my results have been better doing it.

Never personally had amazing results with clones...but obviously that is nothing wrong
with cloning, just my process there.

Ultimately, I think most people try it all, every which way and settle on what works for them. I think that's
what we are all doing whether we recognize that or not.

We can all try it like faht...but none of is faht :lol:


--------------------
Cultivating Panaeolus cyanescens


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Re: Official Copelandia/Panaeolus Thread [Re: Asura] * 1
    #29419463 -

Faht's a :wizard: for sure.

And I can't say I've ran many things out beyond four transfers outside of rare cultures that I'm still trying to fruit :shrug:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29419529 -

Anomalocaris said:
Isnt overnight overkill? Shouldnt take so long for a quart jar. I dont really have a way to measure core temp. I can do 3 hours if you think it needs longer



3 hours probably plenty.  Even better if you can take the jars out and shake them a couple times to move the substrate around during the process to evenly heat everything.  I think there is a broad range of what works in pasteurization.  Less than 140 not hot enough.  Over 170 too hot.  Minimum 2 hours.  Does not seem to be a hard maximum time.  So if in doubt go longer.  I've yet to have trich show up early on with my pasteurized stuff  and I really like pasteurization better than sterilization now.  I was worried about getting it just right.

Sterilization is a no brainer.  15psi in PC for 3 hours kills everything, but pasteurization is better since it preserves the thermophillic organisms that help prevent contamination. Took me a while to break down and try pasteurization, but it is really not that hard.  Err on the side of hotter and longer at first, but not over 170 for sure and you will find your way.  I still like moving things around a bit (with kitchen mitts on) to make sure I get the core hot.  (no core probe here)


--------------------
I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Copelandia/Panaeolus Thread [Re: Hardhead]
    #29419703 -

Asura always a pleasure when you chime in. I read for several months before posting researching pans exclusively, and some of your advice is up there with RR.

I started a thread with some BRF cakes working with a cinctulus or olivacecus or something else, but 2g dry = lvl 2 trip, and I feel the genetics of whatever it is are worth investigating. If you have any advice I'd love to hear it.

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon] * 1
    #29419722 -



Some decent TTBVI plates. All made from the same genetics.
The 2nd one is from a drop of LC. And the last one charcoal agar.

I have a bunch of others on charcoal agar but they grow a bit weird and not symmetrical. Only that one looked nice. Perhaps pan cyan dont like charcoal much. But the charcoal ones I have I also increased the LME to 1% versus 0.5% for the other ones. Perhaps that could also be the reason and they prefer lower nutrients.

I can show the not as nice ones on charcoal agar if someone is interested.

Should I make transfers to other plates from all 4 of these or which one would you pick?


--------------------

Edited by Anomalocaris (12/04/25 08:33 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29419745 -

I would inoculate with any of those plates.  And I would love to see the not nice looking ones with charcoal.  For science :grin:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 2
    #29419754 -



Here they are. What do you think is going on with the not as nice looking ones? They are all transfers from a couple of nice looking and symmetrical plates. I have an FFU and sterilise my cork borer in PC to make transfers and flame it outside my FFU every about 3 transfers.

Either theres some bacterias riding along somehow or its just how some pan cyan grows im starting to think.

And/or maybe they also dont like charcoal that much?


--------------------

Edited by Anomalocaris (12/04/25 09:01 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29419762 -

Why does one plate have 2?

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29419764 -

I get them from time to time, myc fragments


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #29419767 -

Interesting if the fragments were that prolific I would be working that up and keeping track which one it is, as well as the other transfers that looked the best.

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29419769 -

tultedspoon said:
Why does one plate have 2?




Because I dropped the piece of agar on the side at first then moved it in the middle, so some mycelium residue got on the side and started growing there too.


--------------------

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris] * 1
    #29419780 -

See...fragments 😂


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Official Copelandia/Panaeolus Thread [Re: cronicr]
    #29419791 -

Plenty of room left, would a touchdown and then placing the wedge strategically be a good method? Seems like you could easily double your mycelium and cut the number of plates used in half. Instead of dropping in middle, touch 1/3 distance, then place 2/3 distance.

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