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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29418057 -

Isnt overnight overkill? Shouldnt take so long for a quart jar. I dont really have a way to measure core temp. I can do 3 hours if you think it needs longer


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29418064 -

Does this yellow stuff still look healthy?
The other trays also start getting those yellow spots.
Hard to see on the pic, but to my eyes it looks hairy and powdery.
The surface of the casing is moist. Have cut out a little piece and checked with my fingers.
Did the swap test but there was nothing yellow on the swab.


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Re: Official Copelandia/Panaeolus Thread [Re: psychedelicmind]
    #29418092 -

That dont look good my dude. Some contamination.

Once again the gods ram cock in fuckin ass


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29418093 -

Anomalocaris said:
Isnt overnight overkill? Shouldnt take so long for a quart jar. I dont really have a way to measure core temp. I can do 3 hours if you think it needs longer


All it really needs is to be held at those temperatures for an hour.  But that hour starts when the heat has penetrated the glass and the substrate.  Three hours might be enough :shrug:

As to overnight being overkill, we are essentially hot composting. Meaning we are raising the temperature up into a range where thermophilic organisms like to grow and mesophilic organisms die.  If they run out of air you might have problems.  But I have pasteurized things overnight at 140f and it's always been fine.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29418101 -

So its like cooking meat sous-vide. It dont matter how long you cook it for it will be the same.

On ask quick questions thread someone said: A 2015 study in the Brazilian Journal of Microbiology tested hot-water immersion on substrates and found 140°F/60 min reduced Trichoderma by >5 logs, but extending to 160°F/90 min eliminated regrowth in 98% of trials, even with post-treatment inoculation. Anything over 180 risks killing the good stuff.

So thats why I want to go a bit over 150


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Edited by Anomalocaris (12/02/25 07:43 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29418104 -

Yeah as long as it doesn't run out of oxygen. They are living beings so they need to be able to breathe, and if they can't you'll be propagating organisms you don't want that live off co2.

But there's probably enough oxygen even in a sealed quart jar for overnight.  In all honesty though I haven't tested that, I pasteurize in unicorn bags generally.  And use either my insta Pot on warm or pressure cooker at 150f.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (12/02/25 07:44 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29418108 -

tree frog said:
Yeah as long as it doesn't run out of oxygen. They are living beings so they need to be able to breathe, and if they can't you'll be propagating organisms you don't want that live off co2.



Im using the same jars as my grain spawn with some synthetic filter disc so they can breathe


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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29418117 -

I think you will be good then. And yeah I saw that edit just now with the 160 at 90 minute trials.

So, I have generally pasteurized stuff at 140 or 145 for 2 or 3 hours.  But after seeing that post in the quick questions thread I am shooting for more like 150 myself.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29418130 -

FWIW I sous vide my unicorn poo and casing bags at 160F for 6 hours and never see contam in trays for many weeks

I’ll add that I seal em and use them several times over the next two weeks without repasteurizing and don’t have contam issues. If more than a week goes by though I’ll pasteurize again

Edited by philmyco (12/02/25 08:04 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: philmyco] * 1
    #29418242 -

Anyone ever tried to replace the entire composted manure with worm casting? 25% worm casting/75% coir. A grower I know told me to do that. Curious if its better or not. What you think?


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Edited by Anomalocaris (12/02/25 11:10 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Anomalocaris]
    #29418381 -

Hi! I'm struggling with pan cyan LC. I live in country where no one is offering spore prints or swabs, so I bought BVI and TTBVI from one of the local vendors. I didn't have issues with cube LC from them so I thought pan cyan would be ok too.

I started BVI liquid culture and grain bags from the same syringe, and nothing happened, waited 3 weeks, no growth and mckennai from the same order is already colonizing substrate in monotubs.  No grow, nothing. I thougt maybe culture was bad. So I ordered ttbvi but here nothing again. LC seams almost dead. I tried using heating pad after week, I placed it few centimetres under the jar and bags, because a temperature in room is about 20-21C, reached about 25C on jar for 5 days but nothing grows.

So here is my question- is it possible too low temperature killed it?

(Im going to make some agar plates with the rest of it to check, but thought LC would be fine if cube was fine :frown: )

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Re: Official Copelandia/Panaeolus Thread [Re: kamylka]
    #29418396 -

They'll colonize at 20-21c.

And for reference I keep my cultures in a 50f mini fridge and they'll still move.  So unless they got close to freezing on your front porch or something, it's unlikely that temperature was the issue.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29418483 -

Thank you! So I will definitely move on agar to check it.

Do you know which vendor send spores worldwide?

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Re: Official Copelandia/Panaeolus Thread [Re: philmyco]
    #29418502 -

do you stick a meat thermometer in it and wait till internal hits 160 then count the 6 hours or do you just pop it in there once the water reaches 160 and let it go for 6 hours?

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Re: Official Copelandia/Panaeolus Thread [Re: ihansterx4i]
    #29418507 -





My Pan (TTBVI) shoeboxes have stalled. I started with three and the best one started pinning and then trich got it.

These two I’ve moved into a Martha but don’t have any idea how much humidity I should run into it etc. No trich which is good I guess but nothings really happened in the last 2 weeks. Cased about 2.5 weeks ago with pasteurized jiffy. 50/50 hpoo color mix that was pasteurized as well for the sub.

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Re: Official Copelandia/Panaeolus Thread [Re: ihansterx4i]
    #29418524 -

Hi, 
I'm an inexperienced grower (some cubes 25 years ago, currently growing ochras, and grow p.cyanescens in my garden), and my question is- would you recommend MIB or TTBVI for an inexperienced grower? Is one "easier"? Or is there little difference? I'm not really looking for extreme potency, but if there is an objective difference in quality of experience (I understand this is extremely unlikely) then I would take that into account too.

Apologies if this is a stupid or unanswerable question, but it's almost impossible to search for it.

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Re: Official Copelandia/Panaeolus Thread [Re: kamylka]
    #29418618 -

kamylka said:
Thank you! So I will definitely move on agar to check it.

Do you know which vendor send spores worldwide?


Unfortunately, we can't recommend vendors or mention who we use here.  It helps give them plausible deniability, so it's a long-standing rule of the forum.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: 99.99]
    #29418622 -

@ihansterx4i

The mycelium looks dry.  I would suggest giving them a good mist and dialing in your tent so they don't dry back out.

This recent post is one approach.  And there's good discussion here at the end about oxygen and humidity in tents.

https://www.patreon.com/posts/142299311?utm_campaign=postshare_fan&utm_content=android_share

99.99 said:
It’s been a while since I’ve posted the information on my pan grows
my process uses a Martha greenhouse with a humidifier wich supplies, all the moisture and the fresh air for them
The only problem is the pans have a problem with aborts when they get moisture on them after the pinning process ,so to resolve this, I grow them in aluminum trays with drainage holes in the bottom then I put an oversized plastic lid over them, keep the moisture from falling on the mushrooms, but the humidity and the fresh air is perfect for growing conditions
My substrate is 50% colonized wheat and 50% coir which is a little bit different than what most people use
I then put dry Pete moss for a casing about eighth of an inch thick
The reason I use dry is because the humidity from the Martha will do the right moisture content for the Pete boss
But it gives an extra week or so of not being able to contaminate with trich



My martha set… up humidifier is set for 15 minutes on 15 minutes off. The temperature is set at 80°.





--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (12/03/25 12:53 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: crossfields]
    #29418627 -

crossfields said:
Hi, 
I'm an inexperienced grower (some cubes 25 years ago, currently growing ochras, and grow p.cyanescens in my garden), and my question is- would you recommend MIB or TTBVI for an inexperienced grower? Is one "easier"? Or is there little difference? I'm not really looking for extreme potency, but if there is an objective difference in quality of experience (I understand this is extremely unlikely) then I would take that into account too.

Apologies if this is a stupid or unanswerable question, but it's almost impossible to search for it.


Some lines of bvi are starting to show inbreeding depression.  Both are fast, fruit well, and are plenty strong.

I can't speak for the alkaloid profile, because I only grew MiB be once and I didn't eat it.  But I haven't heard anyone complain about it.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: psychedelicmind]
    #29418628 -

psychedelicmind said:
Does this yellow stuff still look healthy?
The other trays also start getting those yellow spots.
Hard to see on the pic, but to my eyes it looks hairy and powdery.
The surface of the casing is moist. Have cut out a little piece and checked with my fingers.
Did the swap test but there was nothing yellow on the swab.




And since no one else answered this or had an idea on what it is, I'm just going to say I've never seen anything like it before and I imagine I would compost it based on your description.

But it does look like there is a pin coming up on the side, so if you want you could let that mature and get a print.  And that might give you some idea if the rest of the tray is worth trying to save.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Jump to top Pages: < First | | 1990 | 1991 | 1992 | 1993 | 1994 | 1995 | 1996 | 1997 | 1998 | 1999 | 2000 | 2001 | 2002 | 2003 | 2004 | 2005 | 2006 | 2007 | 2008 | 2009 | 2010 | | Last >

Shop: Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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