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Re: Ask Quick Questions, Get Quick Answers [Re: JW123] * 2
    #29345392 -



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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao] * 5
    #29345407 -

The Tao said:
Check the SAB for spiders(I work in the basement), load the SAB, wash my hands and arms up to the elbows, dry with paper towels and get to work.



The spiders just want to learn proper clean work techniques and you're just gatekeeping


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 2
    #29345414 -

Classic shroomery gatekeeping. Good catch Puff.:lol:

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Re: Ask Quick Questions, Get Quick Answers [Re: JW123]
    #29345467 -

This is my first 2nd flush and I think i overdid it with the misting.

The mushrooms are pretty wet inside. The inside of some of the caps are dark blue/black and spongy. Are these safe to consume or starting to rot inside?

They smell ok, but the first flush did not have this much water in them and the caps were white inside.

Thanks

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Re: Ask Quick Questions, Get Quick Answers [Re: JW123] * 2
    #29345470 -

I do store my SAB right side up and keep some of my gear in the SAB. That means that fluff and stuff doesn’t float down into the inverted SAB. I work in the basement of a 1950’s bungalow out in the sticks. Spiders are expected indoors and out. Once they’re large enough to bump the SAB  I pay attention.


Edited by The Tao (09/15/25 10:12 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29345491 -



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wow

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical]
    #29345494 -

The second but if you're going through all the steps to get a good agar plate you might as well just put it to grain :shrug:

Or you could let the plate fully colonize and get a plin


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Re: Ask Quick Questions, Get Quick Answers [Re: tinkscat] * 1
    #29345522 -

tinkscat said:
This is my first 2nd flush and I think i overdid it with the misting.

The mushrooms are pretty wet inside. The inside of some of the caps are dark blue/black and spongy. Are these safe to consume or starting to rot inside?

They smell ok, but the first flush did not have this much water in them and the caps were white inside.

Thanks




That black splotchy look is likely a pseudomonas infection. Eat at your own risk. Some say a dehydration course or making tea will make it safe. Anything affected as much as the pictured fruit id probably personally discard.


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tossed_salad]
    #29345527 -

For various reasons I can't go grain / bulk for the moment sadly.
So BRF it is !

Ty !


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wow

Edited by Tropical (09/15/25 10:58 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical]
    #29345539 -

Any great post about when to transfer agar / when to expect it and such ?
I've found some threads but they show what to expect visualy, but not much about anything else.

Ty


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wow

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Re: Ask Quick Questions, Get Quick Answers [Re: Synack]
    #29345541 -

Synack said:
So I'm making the switch to agar and self-prepped millet jars. I did ~18-hour soak on the millet, drained 15m in a paint strainer bag, loaded wet, and 120m PC cycle (mix of pint and quart jars). I put a 1/4-plate wedge of what seemed to be a clean T1 transfer of RustyWhyte from a clone plate into this pint jar on 9/7, so it's now 8 days later. It's not that the growth is stalled, it does seem to be growing each day, it just seems very fucking slow, which is worrying me. I have some PE that I also put to grain about the same time which is also slow, but it's PE so slow feels normal. But RW doesn't seem like it should be super slow? (I wish I had remembered to take pictures of the plates before I put them to grain)

Should I be worried or STFU and LITFA?






Bump


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Profile Pic is the work of Dan Eder
https://www.artstation.com/artwork/L2nLoK

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Re: Ask Quick Questions, Get Quick Answers [Re: Vivi]
    #29345543 -

Shake it to speed up colonization. If it stalls thats a problem, if it recovers it will speed things up.

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #29345552 -

Stipe-n Cap said:
Shake it to speed up colonization. If it stalls thats a problem, if it recovers it will speed things up.



Thanks Stipe. Will do. I've been hesitant to shake "too early" because then I won't see that pretty myc bloom taking over like half the jar from the single source :smile:

How slow a recovery is too slow? I've shaken mostly-colonized jars/bags and had them recover in a couple days, but this being only like 10% colonized, not sure how quickly I should expect it to go?


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:blackspy:00010010
"When a long train of abuses and usurpations, pursuing invariably the same Object evinces a design to reduce them under absolute Despotism, it is their right, it is their duty, to throw off such Government, and to provide new Guards for their future security."


The Hitchhiker's Guide to the Shroomery <-- This is the way.



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Re: Ask Quick Questions, Get Quick Answers [Re: Synack]
    #29345560 -

Is boiling water for 10 minutes enough to sterilize it ?
Looking to do a swab with this tek https://www.shroomery.org/forums/showflat.php/Number/28649340/fpart/all that says :

Spore print - I like to use a sterile swab dipped in sterile water. Wipe a spot on the print and holding the jar sideways so you don't place your hand over the puck, shove the broken off tip into the puck. This is especially effective for stubborn spores.

ty


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wow

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29345579 -

tryptkaloids said:
tinkscat said:
This is my first 2nd flush and I think i overdid it with the misting.

The mushrooms are pretty wet inside. The inside of some of the caps are dark blue/black and spongy. Are these safe to consume or starting to rot inside?

They smell ok, but the first flush did not have this much water in them and the caps were white inside.

Thanks




That black splotchy look is likely a pseudomonas infection. Eat at your own risk. Some say a dehydration course or making tea will make it safe. Anything affected as much as the pictured fruit id probably personally discard.



Thanks, I've binned these.

Any tips for avoiding this in future?

The first flush was fine

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical] * 1
    #29345583 -

Tropical said:
Any great post about when to transfer agar / when to expect it and such ?
I've found some threads but they show what to expect visualy, but not much about anything else.

Ty



You should transfer as soon as possible to get good uncontaminated samples to use. Let it grow a few days until an area with good mycelium presents itself.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 1
    #29345585 -

You should see recovery in roughly 12 to 24 hours. By recovery I mean the mycelium on the exterior of the grains will become fuzzy again, new growth. If it takes days or weeks to recover, that's a solid indicator for contamination.

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #29345634 -

With this tek : https://www.shroomery.org/forums/showflat.php/Number/28649340/fpart/all
I have steel lids, he advises 2 small holes. But how small ?

thank you !


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wow

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical] * 1
    #29345644 -

is anyone else worried that the increase and continued practice of crossing varieties may result in the loss of more "classic" varieties?

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Re: Ask Quick Questions, Get Quick Answers [Re: Tropical]
    #29345652 -

Tropical said:
With this tek : https://www.shroomery.org/forums/showflat.php/Number/28649340/fpart/all
I have steel lids, he advises 2 small holes. But how small ?

thank you !



You want them big enough for your syringe needle to fit through.

Edited by DERRAYLD (09/16/25 01:51 AM)

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck


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