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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 3,545
Loc: uacious, but can't spel gud

Re: Ask Quick Questions, Get Quick Answers

[Re: 1NFIN8]

1
#29341811 - 09/12/25 04:16 PM (1 year, 1 month ago)

:bigyesnod:

Pretty much sums it up.

If I am just looking for growth patterns (like testing lc), multiples to one plate can work fine. When I plan to take a pretty plate to grain I like to let a single transfer grow out to nearly fill the plate (~75% +) to feel really confident that it is clean.

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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
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Gilmour Hendrix
Village Idiot

Registered: 08/02/25
Posts: 1,689
Loc: Bacta-Mold HQ

Re: Ask Quick Questions, Get Quick Answers

[Re: ReverendMyc]
#29341822 - 09/12/25 04:25 PM (1 year, 1 month ago)

Ok cool! I'm going to do some transfers this weekend to expand, make a master or two, and make sure I have good cultures for grain. Thanks for the info!

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Azule
Stranger

Registered: 09/10/25
Posts: 117
Loc: EU🇪🇺
Last seen: 9 days, 3 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: Gilmour Hendrix]
#29341840 - 09/12/25 04:41 PM (1 year, 1 month ago)

It looks like LME that I bough doesn't dissolve completely in the water. Is filtering it through coffee filter a good idea if I want to make agar plates/LC from it?

Edited by Azule (09/12/25 05:33 PM)

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Wedgie
Maysin

Registered: 07/26/15
Posts: 1,311
Loc: 👻

Re: Ask Quick Questions, Get Quick Answers

[Re: Azule]
#29341869 - 09/12/25 05:18 PM (1 year, 1 month ago)

For one US quart of CVG 2-3 tsp of CaO(H)2 should work for a casing layer
mix for Panaeolus or psilocybe species?

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Nichrome
-675 kPa

Registered: 12/17/18
Posts: 8,963

Re: Ask Quick Questions, Get Quick Answers

[Re: Azule]

1
#29341902 - 09/12/25 05:42 PM (1 year, 1 month ago)

Azule said:

It looks like LME that I bough doesn't dissolve completely in the water. Is filtering it through coffee filter a good idea if I want to make agar plates/LC from it?

Some nice mystery sediment there... Yes running it through a coffee filter would clean it up. running it through a cheese cloth would be a good pretreatment though as that sediment will clog up a coffee filter really fast and you'll be waiting forever unless you have a fancy buchner funnel and a vacuum. You could also settle it in the fridge overnight and use a syringe if you have a big one to pull the clean layer off the top.

Personally I wouldn't worry about it though, it's just grain cells and mush don't mind that at all, I mean fuck, I've used brf to make agar before a number of times.

Unless you are trying to count spores under the scope or something, clear agar is merely aesthetic.

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Question everything, it's very responsive.

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Shroomster420au
Stranger

Registered: 09/05/25
Posts: 10
Last seen: 11 months, 17 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Wedgie]
#29341903 - 09/12/25 05:44 PM (1 year, 1 month ago)

Good morning :smile:

:smile:

We used old spores from the cleanest paddock that backs onto national park in the border ranges. Being wild, i expect a bit of cleaning up on agar.

Question is how long for growth to appear. Its been 6 days, i think. Its the first month of Spring - temps would be around 16-26c on estimate.

Thank you :smile:

:smile:

i have the 2 agar plates in the pantry behind a sheet to help moderate tempeature, they are in ziplock bags.

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BeefExtreme
Meatkeeper

Registered: 11/03/11
Posts: 14,584
Loc:

Last seen: 11 minutes, 28 seconds

Re: Ask Quick Questions, Get Quick Answers

1
#29341904 - 09/12/25 05:44 PM (1 year, 1 month ago)

my plates are always ugly. just gonna mutilate them anyway.

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Nichrome
-675 kPa

Registered: 12/17/18
Posts: 8,963

Re: Ask Quick Questions, Get Quick Answers

[Re: Wedgie]

1
#29341905 - 09/12/25 05:44 PM (1 year, 1 month ago)

Wedgie said:

For one US quart of CVG 2-3 tsp of CaO(H)2 should work for a casing layer
mix for Panaeolus or psilocybe species?

2-3 tsp of quick lime would significantly raise the pH of one quart of casing. Probably much further than you want.

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Question everything, it's very responsive.

Culture Storage

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Nichrome
-675 kPa

Registered: 12/17/18
Posts: 8,963

Re: Ask Quick Questions, Get Quick Answers

[Re: BeefExtreme]
#29341907 - 09/12/25 05:45 PM (1 year, 1 month ago)

BeefSupremeJr said:

my plates are always ugly. just gonna mutilate them anyway.

:cantarguewiththat:

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Question everything, it's very responsive.

Culture Storage

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Azule
Stranger

Registered: 09/10/25
Posts: 117
Loc: EU🇪🇺
Last seen: 9 days, 3 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: Nichrome]
#29341935 - 09/12/25 06:27 PM (1 year, 1 month ago)

Ah, I know the mystery. I bought malt instead of malt extract.

Thanks for the advice :smile:

:smile:

Edited by Azule (09/12/25 06:27 PM)

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Wedgie
Maysin

Registered: 07/26/15
Posts: 1,311
Loc: 👻

Re: Ask Quick Questions, Get Quick Answers

[Re: Azule]
#29341939 - 09/12/25 06:29 PM (1 year, 1 month ago)

Hey I wonder if malt would work instead of malt extract...
just mind you the tannins and protein and such in the malted grain (grain is it?) potentially causing cloudiness.
I think.

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Nature Reclaims All.

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cronicr

Registered: 08/07/11
Posts: 65,681
Loc: Van Isle

Last seen: 8 hours, 9 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Wedgie]

1
#29341983 - 09/12/25 07:02 PM (1 year, 1 month ago)

Clear lc is only there to satisfy those with ocd

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GenesisCorrupted
Cowboy, Cultivator, Dude

Registered: 08/01/23
Posts: 33,860
Loc: PNW

Re: Ask Quick Questions, Get Quick Answers

[Re: cronicr]

1
#29341993 - 09/12/25 07:09 PM (1 year, 1 month ago)

It's nice watching it clear up.
You're not left with so many questions.

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mushboy
modboy

Registered: 04/24/05
Posts: 41,034
Loc: the woods

Re: Ask Quick Questions, Get Quick Answers

[Re: GenesisCorrupted]

3
#29341996 - 09/12/25 07:12 PM (1 year, 1 month ago)

i made a 500ml lc with 10g of brf by accident that shit looked like and poured like grits.

grew shrooms tho. i do like a clear lc. the clearer the broth gets as it colonizes has usually been a good sign for me.

always test on agar
:thaaannks:

:thaaannks:

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:hypnotoad:

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Mushboy likes these teks : Making shroom tea
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Sneeb
Stranger

Registered: 03/25/25
Posts: 1
Last seen: 9 months, 6 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: mushboy]
#29342163 - 09/12/25 09:42 PM (1 year, 1 month ago)

Hi!

I've isolated this tub, but wanted an opinion. Is this Trich?

Two others from the same agar plate.

Edited by Sneeb (09/12/25 09:45 PM)

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Vivi

Registered: 01/10/25
Posts: 59
Loc: Black Mage Village

Re: Ask Quick Questions, Get Quick Answers

[Re: Sneeb]
#29342189 - 09/12/25 10:09 PM (1 year, 1 month ago)

Oh no!! This seems like trychoderm.. the green 😭

And maybe the white isn't mych after all, but cobweb? 😭

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Edited by Vivi (09/12/25 10:09 PM)

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mushboy
modboy

Registered: 04/24/05
Posts: 41,034
Loc: the woods

Re: Ask Quick Questions, Get Quick Answers

[Re: Vivi]

1
#29342190 - 09/12/25 10:09 PM (1 year, 1 month ago)

nah thats toast. compost time.

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:hypnotoad:

:hypnotoad:

Making Liquid Culture : Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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boxtrotalpha
rascal bandit mushroom slave

Registered: 06/17/24
Posts: 2,616
Loc: The rings of Saturn
Last seen: 10 months, 15 days

Re: Ask Quick Questions, Get Quick Answers

[Re: mushboy]
#29342219 - 09/12/25 10:25 PM (1 year, 1 month ago)

Quote:

mushboy said: looked like and poured like grits.

Bro, I'm a bottle of wine in right now and that shit had me snort laughing on my deck. Neighbor probably thinks im insane

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Where to start?
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tossed_salad
Some dude on the internet

Registered: 05/02/23
Posts: 235
Loc: USA

Last seen: 1 hour, 43 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Vivi]
#29342228 - 09/12/25 10:33 PM (1 year, 1 month ago)

Vivi said:

Oh no!! This seems like trychoderm.. the green 😭

And maybe the white isn't mych after all, but cobweb? 😭

The white looks good homie but definitely toss that jar, she's a goner

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the_chosen_one
burden of desire

Registered: 09/11/06
Posts: 5,873
Loc: 1984

Re: Ask Quick Questions, Get Quick Answers

[Re: tossed_salad]
#29342310 - 09/12/25 11:47 PM (1 year, 1 month ago)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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