callmehippie said:

Let me re phase a lil.

I'm saying I know that I have to remelt it in order to do any cleaning out. Wasn't sure if going in the microwave with all that bacteria was the best idea or not?

I don't want my microwave to be left with a bad smell now or foreseeable future is what I'm worried about. Nobody wants to make food and something that stinks Just For Peace of mind.


And then wasn't sure if I did just put it in the PC.

Would  I have to throw it away after or at that point is it good to use again?

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dinosaurs said:

hello, back with plate pix and questions.  two plates, both transfers from the same parent plate.  in pic 1 the inoculant was transferred with the mycelium facing up, and in pic 2, the inoculant was transferred with the mycelium facing down.  in pic 3, you're seeing the reverse side of plate 2 (pic 2), and in pic 4 you're seeing the reverse side of plate 1 (pic 1).

are the bottom side dots contam?  I was excited about those plates because I thought they were looking good.  sons of bitches cooties.

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tryptkaloids said:

Ive always melted it in a pot with a trivet or towel down then just dumped it out/in the compost

A water bath can take forever to melt agar.

Quick question on topic, anyone ever have/use a pre-hot spigot? Idek what to call em, its like a second sink nozzle but connected to a hot water tank thats always like 120f

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It's called an Instant Hot. It's usually much closer to boiling. Mine sits at about 195F (any higher and it has a tendency to boil in the tank which makes it sputter).  And I have definitely used it when cleaning out my media bottles after a pour, it's great.

But I usually use it to make coffee (water for americano) and tea. It's life-changingly convenient compared to having to boil water

https://www.homedepot.com/p/InSinkErator-Invite-HOT150-Series-1-Handle-6-25-in-Instant-Hot-Water-Dispenser-Tank-with-Faucet-in-Chrome-H-HOT150C-SS/300226753?source=shoppingads&locale=en-US

dinosaurs said:

dinosaurs said:

hello, back with plate pix and questions.  two plates, both transfers from the same parent plate.  in pic 1 the inoculant was transferred with the mycelium facing up, and in pic 2, the inoculant was transferred with the mycelium facing down.  in pic 3, you're seeing the reverse side of plate 2 (pic 2), and in pic 4 you're seeing the reverse side of plate 1 (pic 1).

are the bottom side dots contam?  I was excited about those plates because I thought they were looking good.  sons of bitches cooties.

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any other thoughts on this?  sorry to bump it but it got buried pretty fast.

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tryptkaloids said:

Ive always melted it in a pot with a trivet or towel down then just dumped it out/in the compost

A water bath can take forever to melt agar.

Quick question on topic, anyone ever have/use a pre-hot spigot? Idek what to call em, its like a second sink nozzle but connected to a hot water tank thats always like 120f

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tryptkaloids said:

dinosaurs said:

dinosaurs said:

hello, back with plate pix and questions.  two plates, both transfers from the same parent plate.  in pic 1 the inoculant was transferred with the mycelium facing up, and in pic 2, the inoculant was transferred with the mycelium facing down.  in pic 3, you're seeing the reverse side of plate 2 (pic 2), and in pic 4 you're seeing the reverse side of plate 1 (pic 1).

are the bottom side dots contam?  I was excited about those plates because I thought they were looking good.  sons of bitches cooties.

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any other thoughts on this?  sorry to bump it but it got buried pretty fast.

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I actually did think about this... is it possible the line is a cut in the agar?

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dopesauce said:

Love this thread. Gotta love the variety of opinions and options.

I did some HG PDYA MS plates. Instead of streaking I just gave the plate a gentle shimmy. I’ve now done a few transfers and gotten some pretty clean isolates which I just put into some millet.
The goal with getting a nice isolate is so that you get a nice even canopy?

I just noticed that a couple of the MS plates have started to pin. Should I grab some tweezers and just transfer those pins to a clean plate? Clearly those strains are the fastest/hardiest ones?
If I put the MS plates to grain and then to bulk I would most likely end up with an unfull canopy I imagine however I would have the most genetic options to choose from.

Planning on doing this round and then cloning the best fruits moving forward.

Is there a significant difference in how long it takes for a pint vs a quart to fully colonize? I’m planning to do some g2g with them.

Is there an easy way to store prepped substrate. I was thinking to rehydrate coir with a bucket tek, then putting it in a trash bag and tie it up. Would it stay good for a couple weeks like this.

Sorry that’s a million questions at once. Appreciate your input.

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I have gotten some of my better canopies from MS plates. As far as pint vs quart the smaller amount should colonize faster but it depends on your genetics and conditions also. Bucket tek coir will remain hydrated in a bucket or cooler with a good sealing lid. I use both types and keep coir in them for weeks. Even if it goes a little dry just add more water to bring it back to field capacity. 

leproos said:

Question from a noob here..

My cat can choose to order spore prints or LC. The prints would be safer with shipping but i think the LC would be much more convient. He has some experience with agar and all the stuff is here. Still i am leaning towards LC..

Can u please convince my cat why spore prints are the better choice better?

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tree frog said:

leproos said:

Question from a noob here..

My cat can choose to order spore prints or LC. The prints would be safer with shipping but i think the LC would be much more convient. He has some experience with agar and all the stuff is here. Still i am leaning towards LC..

Can u please convince my cat why spore prints are the better choice?

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Since your cat is the one growing mushrooms, it should get it's own account so he can ask us himself.

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cybork said:

AZ Starman2112 said:

Can you send a plate to grain that is older, has pinned and spored and is still contam free?

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Yes you can.

As long as the goal is not to replicate the specific clone (as you now also have spores).

Maybe you have to do apply some revival strategy, but probably not.

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leproos said:

tree frog said:

leproos said:

Question from a noob here..

My cat can choose to order spore prints or LC. The prints would be safer with shipping but i think the LC would be much more convient. He has some experience with agar and all the stuff is here. Still i am leaning towards LC..

Can u please convince my cat why spore prints are the better choice?

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Since your cat is the one growing mushrooms, it should get it's own account so he can ask us himself.

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Unfortunatly he does not have the password of the laptop.

my thougt is if i have to put LC to agar then why not just put spore print to agar. or is the LC better in terms of clean or potency?

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That's better fam.  Blaming your cat for your felonies won't hold up in court anyway

An LC is faster in terms of colonization speed and not having to wait for things to germinate.  But as you mentioned, you want to check it on agar first.  And if it's not clean you'll have to deal with getting a new one from the vendor.  The shelf life is also less.  But it's likely a more isolated culture, and likely to produce well.

A print you have to wait for it to germinate.  And you might not get a great culture your first attempt.  But, you can isolate multiple cultures from the same germ plate and store the best ones for future production.

And a print, when properly stored, can last a very long time.  Allowing you to go back to it again and again.

Personally, I prefer prints.  Even if I know the vendor personally and can get LCs for free.

One plus of LCs is you can start aio bags with them.  But, AIO bags tend to be a headache too.  And aren't something a lot of us recommend.

Long story short, this hobby is built on patience.  Get a print imo.

Magspy said:

Hey guys, I accidentally left a batch of mushrooms in my dehydrator for a week. They look fine, but does anyone know how badly their potency will be affected by this?

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Like with the dehydrator on? The potency will not be affected at all. You cannot over dehydrate the mushrooms.

@tree frog
Since your cat is the one growing mushrooms, it should get it's own account so he can ask us himself.

For that comment.