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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: noomer]
    #29292545 -

noomer said:
I bought a 5 pack of rapid rhizo agar plates from Midwest grow kits and swabbed two plates on the 17th. There’s absolutely no growth of any kind. Should I see anything by now? I’m new to agar



Personally I’m not a big fan of mwgk at all. Or any “special formula plate” but spores can take their sweet ass time sometimes. Give it 2 weeks before you consider it a dud imo.


--------------------
Face failure head on and learn something from it
The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: misterswisher] * 2
    #29292548 -

Definitely make your own plates, and I wouldn't buy a damn thing from midwest.

I cannot overemphasize how easy it is to pour clean plates in a SAB.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29292549 -

Thank you both. I’ll start pouring my own plates after this. I’ll give it a couple weeks and see what they do


--------------------
~Wherever you go in life, there you’ll be~




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Re: Ask Quick Questions, Get Quick Answers [Re: noomer] * 1
    #29292556 -

Also, swabs are much harder to germinate from than a print.  I personally prefer prints.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112]
    #29292570 -

AZ Starman2112 said:
Ok here’s another question about those ochras that grew while I was away. They smelled awesome when I harvested them last night. The tub was healthy. No contam visible. However, tonight I noticed when I took last nights harvest out of the dehydrator the stems/stipes were a bit soft. I broke one open and it was a blueish green inside. It prompted me to look at a few others and all were the same. I went to look at the ones that were harvested by my shroom sitter while I was away on vaca and they were also the same. This is what I found inside. Has anyone come across this before? What is it? I’m assuming it is some kind of mold and these are all trash?










Yeah they're fine. I've had some look like that before but they were more of a gray almost like decaying inside of bone look. But they were all fine I ate them and tripped hard no sickness or adverse effects.I've had a few batches like that.




--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29292579 -

Any tips on germinating from swabs?


--------------------
~Wherever you go in life, there you’ll be~




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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29292583 -

Mr Piggy said:
Definitely make your own plates, and I wouldn't buy a damn thing from midwest.

I cannot overemphasize how easy it is to pour clean plates in a SAB.


This is my experience so far fwiw.


--------------------
You can ignore the truth to your final breath. But trust me, it will meet you there to be ignored no longer.

The answer to your question is almost always 42
“The answer is always grow more shroom.” Mr Piggy
- Those two answers coexist peacefully.

Quick Sterilization Reference for Altitude

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Re: Ask Quick Questions, Get Quick Answers [Re: noomer] * 2
    #29292586 -

noomer said:
Any tips on germinating from swabs?



You could use this Here

Revs Pristine BRF Puck tek


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: noomer] * 1
    #29292600 -

noomer said:
Any tips on germinating from swabs?


This is my preferred method




Streak it, and then wedge the swab head under the agar. I did three straight lines, then wedged the swan under the agar





Or don't, and just wedge the swab under/into the plate

I'm speaking strictly for cubensis

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Re: Ask Quick Questions, Get Quick Answers [Re: noomer] * 1
    #29292602 -

noomer said:
Any tips on germinating from swabs?


Use 2 or 3 plates. Wipe the swab across all of them, then jam it INTO the agar on the last one and leave it there. Often for me that's the only one that grows.

It can help to put a drop of PCed distilled water on the swab first.

I like spore prints SO much more than swabs. More to work with, much better chance of germination.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 3
    #29292615 -

callmehippie said:
noomer said:
Any tips on germinating from swabs?



You could use this Here

Revs Pristine BRF Puck tek


+1 for brf pucks. I'll usually run them over a plate or two and then stab it into a puck as a backup.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29292691 -

callmehippie said:
AZ Starman2112 said:
Ok here’s another question about those ochras that grew while I was away. They smelled awesome when I harvested them last night. The tub was healthy. No contam visible. However, tonight I noticed when I took last nights harvest out of the dehydrator the stems/stipes were a bit soft. I broke one open and it was a blueish green inside. It prompted me to look at a few others and all were the same. I went to look at the ones that were harvested by my shroom sitter while I was away on vaca and they were also the same. This is what I found inside. Has anyone come across this before? What is it? I’m assuming it is some kind of mold and these are all trash?










Yeah they're fine. I've had some look like that before but they were more of a gray almost like decaying inside of bone look. But they were all fine I ate them and tripped hard no sickness or adverse effects.I've had a few batches like that.





Bro! Thanks for the pics! THAT made me feel much better seeing someone else’s with the same. Although everyone’s answers were awesome as well, the pics definitely put me at ease!

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112] * 1
    #29292768 -

:whathesaid:

All of those were great answers from everyone.  Any of those is a great way to germinate swabs.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] * 2
    #29292869 -

Np. Az.  I had the same concerns as well. That's why I have the pics. I asked the question my first few months in.


Happy to put your mind at ease:sunny:


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 2
    #29292870 -

I picked up one of these sterilizing canisters



a while back and it's just been sitting around. I'm ready to use it though.

I know there's a few people out there I recall that have mentioned they have one.

Just curious if anybody that uses one of thes could  hook me up with any tips and tricks they might have figured out when using one.  so I don't have to do as much trial and error.

If you have one of these do you have some sort of method that you have figured out to make the best plates when using it?

Or is it just as easy as load it up and sterilize it and that's it?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29292883 -

These are the first jars I've done that have gotten this far along. Could anyone let me know if they're looking good/viable and if not, what I might've done wrong? I think I made my first batch too wet; the first image, in the taller jar, is 2-4 weeks more recent than the others but I dried the grain out much more thoroughly. Is the wet, slimy, stuck-to-the-walls/stuck-together look on parts of the other jars a sure sign of bacterial contamination, or could that be purely down to wetness making things sludgy? I don't think any of these are ready to send yet, just curious about how badly they might be screwed up.

(If it matters, these are oats inoculated with cubensis liquid culture. Soaked for a few hours, boiled 25-35 mins, pressure cooked at 15 psi for 2 hours, kept at 20-28 Celsius/68-82 Fahrenheit, 6mm breathing holes with polyfill or filter stickers depending on the jar.)




--------------------
it is in your self-interest to find a way to be very tender

Edited by chillshroom (07/24/25 06:35 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: chillshroom]
    #29292886 -

Bacterial infection for the most part, that jar with the red lid is going to smell like bacterial soup.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #29292893 -

Damn, I wonder what I'm doing wrong to get so much bacterial contam. I had 5 go moldy before this, too. Before trying jars I did 19 instant rice bags and 17 were successful, and the culture doesn't look bacterial on agar that I can tell, so maybe it's the filters or the way I set up the jars and sealed the injection port. Or maybe I should sterilize at higher pressure or for longer--what's the risk of going too hot for too long, does it leave telltale signs?


--------------------
it is in your self-interest to find a way to be very tender

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Re: Ask Quick Questions, Get Quick Answers [Re: chillshroom] * 2
    #29292898 -



Can I get a health check? Fully colonized 7/20.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: chillshroom]
    #29292918 -

chillshroom said:
Damn, I wonder what I'm doing wrong to get so much bacterial contam. I had 5 go moldy before this, too. Before trying jars I did 19 instant rice bags and 17 were successful, and the culture doesn't look bacterial on agar that I can tell, so maybe it's the filters or the way I set up the jars and sealed the injection port. Or maybe I should sterilize at higher pressure or for longer--what's the risk of going too hot for too long, does it leave telltale signs?


So I've had some contam issues since recently trying oats as well. I'm thinking 75% of my issue is stemming from the oats not being dry enough when I jar and PC them. Water allows bacteria to move around so the more there is the more mobile the bacteria can be, the more mobile they are the more resources they have available to reproduce with


--------------------
Where to start?

Are your plates clean?


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Shop: MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   Mushroom-Hut Mono Tub Substrate   Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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