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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #29285306 -

mushboy said:
chris77 said:
tryptalkaloids said:
Agar can be steam sterilized



tx trypt, it sure can! question is how long to steam sterilize  in a regular pot when the agar is in the typical round screw lid bead containers…
90 min? overkill?



way overkill. liquids are great conductors so you only need to steam or boil the agar for like 30min.



the containers are quite thick.. will give em 45 to be sure
havent been without a PC for too long :wink:
tx mush


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Ask Quick Questions, Get Quick Answers [Re: nocap] * 1
    #29285308 -

Y
nocap said:
I guess I just don’t understand the need to cook a second time.

I am either confident in the success of my initial grain prep tek, or I am willing to spend $5 and try again.

Some of these guys grow stones in jars for 6months without contams.  So I should be able to trust idle jars on the shelf to stay idle for a few weeks or a couple months.



Actually mentioning the stones kinda makes it all make sense. I didn't think of this before tbh and just thought it's best to be safe rather than sorry.


--------------------

Edited by SkanskKorv (07/18/25 01:03 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: chris77] * 8
    #29285310 -

dont run outa water:cop2:

ive told this story before but i think its due again..

20ish yrs ago i pasteurized 4 or 5 rounds hpoo inside one of those big black lobster steamer pots and i stashed the pot in my dads garage without cleaning it out.

couple months later i come over and smell the most rank shitty fish smell and see my dad cooking crabs using that same pot.

i asked him 'uhh you cleaned that out after sitting in the garage right???' my dad stubborn as fuck says 'no! why would i? my garage is clean!!'

he proceeds to eat every last crab leg to prove to me that nothing was wrong and i was imagining the smell and green stains on the crab meat.

he died a few weeks ago and i never told him the truth:lol:

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Re: Ask Quick Questions, Get Quick Answers [Re: chris77] * 1
    #29285311 -

I feel like overkill would be hard to achieve with liquids


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: nocap] * 2
    #29285313 -

nocap said:
I guess I just don’t understand the need to cook a second time.

I am either confident in the success of my initial grain prep tek, or I am willing to spend $5 and try again.

Some of these guys grow stones in jars for 6months without contams.  So I should be able to trust idle jars on the shelf to stay idle for a few weeks or a couple months.



I've been leaving oat jars on the shelves for a few months before I use them, and so far, I haven't encountered any issues during the year I've been learning. When I prepare grain jars, I typically run about 20 to 30 quart jars in a day, and I appreciate how they dry out a little over time while they sit. I've noticed that nearly all the cube varieties and Lion's Mane mushrooms I’ve sent to these jars tend to colonize more quickly on the oats that have been sitting for a while and have developed a slightly dried outer layer vs a day or two after running them.




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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy] * 1
    #29285318 -

mushboy said:
dont run outa water:cop2:

ive told this story before but i think its due again..

20ish yrs ago i pasteurized 4 or 5 rounds hpoo inside one of those big black lobster steamer pots and i stashed the pot in my dads garage without cleaning it out.

couple months later i come over and smell the most rank shitty fish smell and see my dad cooking crabs using that same pot.

i asked him 'uhh you cleaned that out after sitting in the garage right???' my dad stubborn as fuck says 'no! why would i? my garage is clean!!'

he proceeds to eat every last crab leg to prove to me that nothing was wrong and i was imagining the smell and green stains on the crab meat.

he died a few weeks ago and i never told him the truth:lol:



tx for sharing
:taminated:


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog] * 2
    #29285322 -

Oats are high in lectin and phytate content which prevent enzymatic degradation of the seeds. These lectins break down with heat and time.

longer shelf time = less lectin/phytate content = easier enzymatic digestion

Also oats are typically harvested using glyphosate or similar desiccant so all the oats are uniform at harvest.  These chemicals vape off over time.


--------------------
Question everything, it's very responsive.


Culture Storage

Edited by Nichrome (07/18/25 01:19 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29285331 -

Nichrome said:
Oats are high in lectin content which prevents enzymatic degradation of the seeds. These lectins break down with heat and time.

longer shelf time = less lectin content = easier enzymatic digestion

Also oats are typically harvested using glyphosate or similar desiccant so all the oats are uniform at harvest.  These chemicals vape off over time.



Easier enzymatic digestion = easier digestion for the myc? Is that the takeaway here?


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: nocap] * 3
    #29285333 -

nocap said:
SkanskKorv said:
Really? I've had TCs telling me to pc jars again and I've also done so thanks to this and those jars have been just as good as the other ones tbh.



I could be wrong.  :shrug:  Either way though, grain is cheap and time is not.

If the jars were prepared correctly the first time, then they don’t need cooked again because they are sterile and will remain so.

If they weren’t prepared correctly, a second cook is unlikely to save them better than just giving it another go from scratch.

I like to think of it as best chance for success rather than *could* it work out.  IRL sterility is a probability and we are all playing the numbers.



Both substrate and grain can be overcooked.

Eventually the sugars change form when heat treated.  And they become less bioavailable to the mycelium.

I'm sure this point is going to vary, as different grains have different sugars, as well as different structures.

I first came across that concept in one of Stamet's books when he was talking about sawdust blocks.  But I have encountered it myself trying to re-sterilize aio jars.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 3
    #29285411 -

:whatshesaid:

I have pictures here somewhere to back this up. Did some side by side tests with single and double pc'ed grains and the over cooked grains either failed to colonize or struggled and took forever to colonize. Running out to feed the fam or I would dig up the pix.


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
    #29285478 -

Are APE Revert more potent mushrooms then other strains? :mushroom2:♥️

Edited by killreflection (07/18/25 03:42 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc] * 1
    #29285492 -

ReverendMyc said:
:whatshesaid:

I have pictures here somewhere to back this up. Did some side by side tests with single and double pc'ed grains and the over cooked grains either failed to colonize or struggled and took forever to colonize. Running out to feed the fam or I would dig up the pix.



On the other hand I hydrate popcorn like cooking dry beans.  Forty minutes in the IP.  And then a full PC cycle.

But they're submerged in water during hydration, so maybe that stabilizes the sugars.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 1
    #29285505 -

"AGEs, HAAs, AA, 5-HMF, and PAHs are toxic substances that are produced during the thermal processing of certain foods."


--------------------
Question everything, it's very responsive.


Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29285508 -

tree frog said:
ReverendMyc said:
:whatshesaid:

I have pictures here somewhere to back this up. Did some side by side tests with single and double pc'ed grains and the over cooked grains either failed to colonize or struggled and took forever to colonize. Running out to feed the fam or I would dig up the pix.



On the other hand I hydrate popcorn like cooking dry beans.  Forty minutes in the IP.  And then a full PC cycle.

But they're submerged in water during hydration, so maybe that stabilizes the sugars.



That is the golden ticket.


--------------------
Question everything, it's very responsive.


Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29285517 -

Hi, I'm new here. I have 5 LCs:
- PE
- Enigma
- AMVP
- ALT7
- Orcha

Temperature at my place is like 28-30C.
Can I grow all 5 using PF TEK and fruiting in the same water tub?

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Re: Ask Quick Questions, Get Quick Answers [Re: Sandlark]
    #29285524 -

As long as the LC is good there is no reason multiple varieties would mind sharing space like that.


--------------------
Question everything, it's very responsive.


Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29285529 -

Alright thanks. I was just worried that the temperature might be too high for some of them, or that they might require different amount of FAE.

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Re: Ask Quick Questions, Get Quick Answers [Re: Sandlark] * 2
    #29285589 -

They all do have different requirements but I'm sure you do too and there is a layered reasoning behind your design involving space, time, effort, and stealth.  Generally speaking, a water tub is middle ground for conditions with all those varieties.


--------------------
Question everything, it's very responsive.


Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Sandlark]
    #29285610 -

Sandlark said:
Alright thanks. I was just worried that the temperature might be too high for some of them, or that they might require different amount of FAE.



30c is pretty high.  You might have more luck fruiting those in a cooler season if that's an option, as they all do well at like 22-24c.

Tropical pans would do better.  Such as cyans.  But they like a casing and generally some nitrogen.

Here's a cake recipe for pans.  You could do this in a water tub.

https://www.shroomery.org/forums/showflat.php/Number/18357343


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 1
    #29285621 -

Or just run an ac


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
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Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Myyco.com APE Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Grow Bags


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