Dkeppel said:

Whatever the species I'm working with, I'm looking for fast organised growth with a clearly defined leading edge, assuming you want fruit and not sclerotia when working with species in section mexicanae, I will avoid the mycelium with a pigment and select for the white stuff.

It's a little difficult when I don't know exactly which species each plate is and from only a single photo without a backlight but I will do my best

Plate 1:

I am assuming this is a cube.

If those spots are sediment, you could probably let this culture to continue to grow out and send it to grain, assuming it continues to grow organised with a clearly defined leading edge.

If you want to transfer, everything from about 1 to 8 o'clock looks good.


Plate 2:

This looks like semps? Or is it the Pan you mentioned?

It's really hard to see what's going on but I think the bottom culture looks better, 11 to 4 o'clock, hold it up to a back light and check the growth is organised etc.


Plate 3:

Assuming semps again? Maybe this is a pan?

I don't like how it's growing, possibly has bacteria riding along, if you want to try save it, give cabin sequesting a try, growth at about 9 o'clock looks the best I think.


Plate 4:

Looks section zapotecorum. Semps

Looks like you have an orange dot close to the transfer, 7 to 11 o'clock looks like the nicest growth.


Plate 5:

This looks like your germination plate so I will assume I should ignore this one.


Plate 6:

Maybe this one is the sub?

This looks sketchy, you might want to give cabin sequestering a try, looks like some nice growth between 12 to 1 o'clock and about 8 to 9 o'clock.


Plate 7:

These next plates all look section mexicanae so I'll assume they are tampanensis since you mentioned tamps.

Top culture, I cant see what's under the glad wrap but I don't like much of what I can see, maybe around the 3 o'clock region.

Right-hand culture, looks like 4 to 5 o'clock has some nice growth.

Bottom culture, this is the best looking culture on the plate, 4 to 10 o'clock section looks good.

Left-hand culture, section between 8 to 9 o'clock.


Plate 8:

Looks like another possible bacteria riding along culture, again cabin sequesting if you want to try save it, about 10 o'clock I think.


Plate 9:

Again, it is hard to see what is going on where the glad wrap is covering the mycelium but these all look clean from here.

Maybe let them continue to grow out and if you feel the need to transfer, again hold it up to a back light, look for the fastest organised growth with a clearly defined leading edge and transfer from there.


I hope that helps! Good luck.

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LEGEND!

Mackload said:

my malt extract too, but it dissolves nice. I'm thinking about dissolving the whole jar and trying to math the quantities but math is not my strong suit lol

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Probably better to put it in a blender and break it all up, then store with some desiccant packs. If you add water, things will be able to grow in it, unless you sterilize (and handle under sterile conditions or re-sterilize) or you are careful to keep the water content very low, like a concentrated syrup. I don't know what that threshold is, but for jelly/jam it's something like 40% water content or less.

Depending on how much you have left, you could make a bunch of jars of wort, pressure can them all, and use a whole jar at a time for agar or LC media. But it's still probably less work to leave it dry.

Fullcircle1 said:

Really slow APER transfer I took from MSS plate. Been almost 2 weeks, think I may transfer this to a new batch of agar I made last week. Maybe it's the agar?

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tryptkaloids said:

Fullcircle1 said:

Really slow APER transfer I took from MSS plate. Been almost 2 weeks, think I may transfer this to a new batch of agar I made last week. Maybe it's the agar?

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Looks like theres a bacterial biofilm surrounding the culture

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ClearLakeBoy said:

Looks like it made it, took a spot from the top left. And a couple of other plates. These are only my second plates, feels good to get them going.
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ClearLakeBoy said:

Looks like it made it, took a spot from the top left. And a couple of other plates. These are only my second plates, feels good to get them going.
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holy smokes nice job