Here is my greatly shortened HPLC results post. Taken from here:
https://www.shroomery.org/forums/showflat.php/Number/28462347
There is much more detail given in my full thread, grow conditions, substrate etc... but the basic HPLC data is here. All testing done through Altitude Consulting, Denver, CO.
Bungmurphy said: The samples I tested 9/2024, were weighed and packaged at the same time as the samples that were tested in 9/2023.
First test were done 9/2023 and are in GREEN.
One-Year storage tests from 9/2024 are in RED.
#1 2020/2021 A This sample contains a random mix of harvests from 2020 & 2021; These fruits are from spore prints I take year after year. Originally a mix of legacy cultures, B+, Mazatec, Golden Teacher etc... Test results: Psilocybin = 4.6 mg/g Psilocin = 0.6 mg/g Baeocystin = 0.2 mg/g Total: 5.4 mg/g
#2 2020/2021 B This second sample contains a random mix of harvests from 2020 & 2021; These fruits are from spore prints I take year after year, Originally a mix of legacy cultures, B+, Mazatec, Golden Teacher etc... Additional sample set aside for testing in 1 year Test results: Psilocybin = 4.2 mg/g Psilocin = 0.6 mg/g Baeocystin = 0.3 mg/g Total: 5.1 mg/g
---- SAMPLE 2020/2021 C Aeruginascin = 0.026 mg/g Baeocystin = 0.247 mg/g Psilocin = 0.407 mg/g Psilocybin = 4.116 mg/g Total Alkaloids = 4.796 mg/g
%loss = (5.25 (Average of previous 2 samples)- 4.796)/5.25 = 8.6% loss ----
#3 2022 A This sample contains a random mix of harvests from January-June 2022; These fruits are from spore prints I take year after year, Originally a mix of legacy cultures, B+, Mazatec, Golden Teacher etc... Sealed in June 2022, never opened until now (9/2023). Test results: Psilocybin = 3.2 mg/g Psilocin = 0.8 mg/g Baeocystin = 0.2 mg/g Total: 4.2 mg/g
#4 2022 B This second sample contains a random mix of harvests from January-June 2022; These fruits are from spore prints I take year after year, Originally a mix of legacy cultures, B+, Mazatec, Golden Teacher etc... Sealed in June 2022, never opened until now (9/2023). Additional sample set aside for testing in 1 year Test results: Psilocybin = 2.9 mg/g Psilocin = 0.7 mg/g Baeocystin = 0.2 mg/g Total: 3.8 mg/g
---- SAMPLE 2022 C Baeocystin = 0.126 mg/g Psilocin = 0.390 mg/g Psilocybin = 2.334 mg/g Total Alkaloids = 2.850 mg/g
%loss (4-2.850)/4 = 28.75% loss ----
#5 BLOB 2023 A This is a sample of first flush of PE blobs Test results: Psilocybin = 8.3 mg/g Psilocin = 1.4 mg/g Baeocystin = 0.2 mg/g Total: 9.9 mg/g
#6 PENIS 2023 A This is a sample of Penis Envy Test results: Psilocybin = 7.4 mg/g Psilocin = 1.0 mg/g Baeocystin = 0.2 mg/g Total: 8.6 mg/g
16 quart "bootbox" of #6 PENIS 2023 A:

#7 REGULAR 2023 A This is a sample of REGULAR fruits from spore prints I take year after year, originally a mix of legacy cultures, B+, Mazatec, Golden Teacher etc.... Test results: Psilocybin = 4.4 mg/g Psilocin = 0.8 mg/g Baeocystin = 0.3 mg/g Total: 5.5 mg/g
#8 P.E. SINGLE FRUIT This was a single PE fruit that weighed 2.6g dried. Half of sample put aside for testing in 1 year Test results: Psilocybin = 3.8 mg/g Psilocin = 1.8 mg/g Baeocystin = 0.0 mg/g Total: 5.6 mg/g
---- SAMPLE PE Single Fruit Psilocin = 0.599 mg/g Psilocybin = 1.562 mg/g Total Alkaloids = 2.161 mg/g %loss = (5.6-2.161)/5.6 = 61.4% loss ----
#9 CAPSULES OO Capsules were made with coffee grinder, and capsule maker in June 2023. A total mix of all harvests, PE, regular (a mix of legacy cultures, B+, Mazatec, Golden Teacher etc...), some Tampanensis/Mexicana... Two capsules put aside for testing in 1 year. Test results: Psilocybin = 8.1 mg/g Psilocin = 1.1 mg/g Baeocystin = 0.3 mg/g Total: 9.5 mg/g
---- SAMPLE 2023 Capsules Aeruginascin = 0.033 mg/g Baeocystin = 0.257 mg/g Psilocin = 0.756 mg/g Psilocybin = 8.056 mg/g Total Alkaloids = 9.102 mg/g
%loss (9.5-9.102)/9.5 = 4.1% loss ----
----
SAMPLE OLD SCRAP MIX JAR: This jar contains scraps, powder, extras from weighing, leftovers...etc. Aeruginascin = 0.091 mg/g Baeocystin = 0.215mg/g Psilocin = 0.686 mg/g Psilocybin = 7.691 mg/g Total Alkaloids = 8.683 mg/g
%loss = No earlier sample to compare
UPDATE 2/16/24 Just got results from latest testing. I took the same PE culture from above picture (spore syringe sourced) and ran it two different ways. I ran 2 56QT mono tubs, both had 4 quarts grain (WBS with some popcorn 5% and rye 5%) were spawned to 10 quarts substrate with 4 quarts of pseudocasing. The difference being one tub got just coir, verm; the second tub got coir, verm and 1/2 cup of dried manure. Substrate was pasteurized in jars on stovetop for 90+ min.
Coir only tub pic:

1. Coir only 56 quart PE tub. 4 quarts grain, 10 quarts substrate, 4 quarts pseudocasing. Days on grain: 33 days Days till first flush (blobs): 35 days First flush yield (blobs, dry): 80.0 grams Days till second flush (penis mushrooms): 14 days Second flush yield (mushrooms, dry): 129.0 grams Third flush yield: 33.0 grams Total yield: 242.0 grams Test results: Sample consists of 1.0 gram of blobs + 1.0 gram fruits. Psilocybin: 8.3 mg/g Psilocin: 0.7 mg/g No other compounds present. Total alkaloids: 9.0 mg/g
2. Manure added 56 quart PE tub. 4 quarts grain, 10 quarts substrate, 4 quarts pseudocasing. Days on grain: 34 days Days till first flush (blobs): 43 days First flush yield (blobs, dry): 69.0 grams Days till second flush (penis mushrooms): 11 days Second flush yield (mushrooms, dry): 122.0 grams Total yield: 191.0 grams Test results: Sample consists of 1.0 gram of blobs + 1.0 gram fruits. Psilocybin: 6.2 mg/g Psilocin: 1.3 mg/g No other compounds present. Total alkaloids: 7.5 mg/g
Tampanensis/Galindoi/ATL #7:

Source labeled: "Tampanesis", in pic, these are the 3 full canopy shoeboxes. Test results: Aeruginascin: 0.6 mg/g Baeocystin: 0.4 mg/g Psilocybin: 10.8 mg/g Psilocin: 0.6 mg/g TOTAL ALKALOIDS: 12.4 mg/g
Source labeled: "Galindoi/ATL#7", this is the shoebox in the front left corner of pic. Test results: Aeruginascin: 1.1 mg/g Norbaeocystin: 0.6 mg/g Baeocystin: 1.5 mg/g Psilocybin: 9.0 mg/g Psilocin: 3.0 mg/g Norpsilocin: 0.4 mg/g TOTAL ALKALOIDS: 15.6 mg/g
Sclerotia/stones/truffles: Test results: A mixture of sclerotia from all grows tested at: 0.4 mg/g psilocybin. No other compounds present. ---
New HPLC results from 2/2025 are in PURPLE:
Jack Frost Test results: Baeocystin = 0.669 mg/g Norbaeocsytin = 0.182 mg/g Norpsilocin = 0.669 mg/g Psilocin = 1.277 mg/g Psilocybin = 6.585 mg/g Total Alkaloids = 9.382 mg/g
Rustywhyte Test results: Baeocystin = 0.103 mg/g Norpsilocin = 0.409 mg/g Psilocin = 3.687mg/g Psilocybin = 2.966 mg/g Total Alkaloids = 7.165 mg/g
Mexicana Strain-A Test results: Baeocystin = 0.899 mg/g Norbaeocsytin = 0.356 mg/g Norpsilocin = 0.299 mg/g Psilocin = 1.960 mg/g Psilocybin = 9.955 mg/g Total Alkaloids = 13.469 mg/g
Edited by Bungmurphy (06/06/25 07:47 PM)
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