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DV8DUG
Hyphal Exhibitionist

Registered: 12/27/23
Posts: 2,155
Loc: Melniboné
Last seen: 1 day, 4 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: MachineElf226]
#29183939 - 04/09/25 07:47 PM (1 year, 5 months ago)

MachineElf226 said:

That little 0.5 cm good enough to go into a 4oz jar of sterile grain? I think I'll wait a bit. I've transferred cultures about 3 times already. I'm out of plates! So I'm like between a rock and a hard place I got no backups till they come in mail so I dunno.. Maybe I need to chance it and transfer it to a grain jar...

You need to wait to grow out a bit before you go to grain.

Jaegur said:

Hi there, agar peeps! I'm incredibly new to doing Agar work, would love any thoughts on what to look out for on these plates and what to do next (transfer to new plates, set them on fire, etc.,). I know at least one looks contaminated, not sure on the others though. These are all from the same cluster of Z-Strain.

Look for the link in my sig.

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Phaethon
Hobbyist

Registered: 07/04/22
Posts: 1,268
Loc: Colorado

Re: AGAR ENVY! (Anything and All things agar!)

[Re: MachineElf226]
#29184066 - 04/09/25 10:26 PM (1 year, 5 months ago)

MachineElf226 said:

That little 0.5 cm good enough to go into a 4oz jar of sterile grain? I think I'll wait a bit. I've transferred cultures about 3 times already. I'm out of plates! So I'm like between a rock and a hard place I got no backups till they come in mail so I dunno.. Maybe I need to chance it and transfer it to a grain jar...

If you have 4 oz jars, you can just make agar media, pour it into them, and sterilize. Lots of other containers (anything glass or polypropylene) work for makeshift reusable plates you can pour before sterilizing too.

Though if you only have half a cm of growth, it's going to be a long time before you truly need to transfer. Assuming it's clean, it'll be fine for at least a couple of months at room temp. No need to sweat having to wait for supplies.

Wasn't clear from your post whether you are waiting on premade plates or petri dishes to pour into, but I'd point out that if you're not pouring your own plates, you are spending a lot more money than you need to.

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Sarnath

Registered: 09/04/24
Posts: 94
Loc: Ultima Thule
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Phaethon]
#29184079 - 04/09/25 10:46 PM (1 year, 5 months ago)

Plates I, II, IV and V six days after transfer. III and some of the unnumbered ones had contaminated spots and some had failed to sprout anything at all (I was too dainty with the inoculation loop, I reckon) so I chucked them. Still, these four ought to be enough to get something to transfer into grains.

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oat bag
a.k.a. 2024-12-11

Registered: 12/11/24
Posts: 183
Last seen: 1 year, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Sarnath]

4
#29184090 - 04/09/25 11:02 PM (1 year, 5 months ago)

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Macaroni
Experimenter

Registered: 10/11/23
Posts: 12

Re: AGAR ENVY! (Anything and All things agar!)

[Re: oat bag]
#29184205 - 04/10/25 04:38 AM (1 year, 5 months ago)

Hello my beautiful people, so I just wanted to ask what do yall think causes these differences in PE growth on agar and which do you think is the preferred one, for reference, both are using the same high nutrient agar mix (1:1 - LME:AgarAgar).

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 14,962
Loc: South Africa
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Re: AGAR ENVY! (Anything and All things agar!)

1
#29184207 - 04/10/25 04:40 AM (1 year, 5 months ago)

Not sure I'd trust either of those to be honest.

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Macaroni
Experimenter

Registered: 10/11/23
Posts: 12

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DERRAYLD]
#29184212 - 04/10/25 04:47 AM (1 year, 5 months ago)

How come? Though these have been sitting for a while already and I'm not planning on using them either way :laugh:

:laugh:

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 14,962
Loc: South Africa
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Macaroni]
#29184214 - 04/10/25 04:51 AM (1 year, 5 months ago)

There's no uniform growth, everything is disorganized.

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Macaroni
Experimenter

Registered: 10/11/23
Posts: 12

Re: AGAR ENVY! (Anything and All things agar!)

[Re: DERRAYLD]
#29184217 - 04/10/25 05:02 AM (1 year, 5 months ago)

Yeah, good point, personally it's been pretty hard for me to get a uniform growth of PE on my plates, for example this is a more recent b+ plate, much more uniform growth comparatively. I get that there is no obvious rhizo growth here, but that depends more so on the plate nutrition, no?

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cybesa
Loving soul

Registered: 05/24/18
Posts: 542
Loc: Europe
Last seen: 7 days, 1 hour

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Macaroni]
#29184552 - 04/10/25 01:22 PM (1 year, 5 months ago)

Isn't that thin transparent line around the main mycelium circle in the center a sign of contam? :eek:

:eek:

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Smellyhobbit
Amateur Wizard

Registered: 04/01/22
Posts: 18,494
Loc: Booger Central
Last seen: 1 hour, 57 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: cybesa]

3
#29184556 - 04/10/25 01:27 PM (1 year, 5 months ago)

Nah that happens sometimes. It’s just a scouting party for the mycelium. Checking things out.

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fungitobewith
Fungi To Be With

Registered: 07/15/07
Posts: 979
Loc: Rolling Stone, PA/Some Place C...

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Smellyhobbit]
#29184566 - 04/10/25 01:40 PM (1 year, 5 months ago)

Interesting how things grow on this wheat water agar. Probably should of diluted it with more water.

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cybesa
Loving soul

Registered: 05/24/18
Posts: 542
Loc: Europe
Last seen: 7 days, 1 hour

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Smellyhobbit]
#29184625 - 04/10/25 02:52 PM (1 year, 5 months ago)

Oh thats great news! I always thought it was a sign of contam.. What a relief. :lol:

:lol:

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fungitobewith
Fungi To Be With

Registered: 07/15/07
Posts: 979
Loc: Rolling Stone, PA/Some Place C...

Re: AGAR ENVY! (Anything and All things agar!)

[Re: cybesa]
#29184650 - 04/10/25 03:34 PM (1 year, 5 months ago)

cybesa said:

Oh thats great news! I always thought it was a sign of contam.. What a relief.

I'm running my WWA back to MEA to check for any signs of contamination but I haven't had really any issues, but clearer media is always nice.

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CranberrySteve
Self taught cranberry enthusiast

Registered: 03/06/25
Posts: 82
Last seen: 3 months, 13 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: fungitobewith]
#29184794 - 04/10/25 06:32 PM (1 year, 5 months ago)

Could I please get an experienced eye over these melmac plates? It's from a vendor LC that was riddled with orange bacteria that I've been cleaning up.

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MachineElf226
Stranger

Registered: 08/12/24
Posts: 395
Last seen: 6 months, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Phaethon]
#29184840 - 04/10/25 07:17 PM (1 year, 5 months ago)

I got some premade plates coming. And soon I'm gonna start working with pouring my own agar because yea with how fast I blew thru them 10 first plates I can see where this is going. I can't afford it lol. I dunno if I even got the resources for agae tho unless they come in the mail I live super rural no place here really sells agar shit unless... Bomgaars has it? I just got some more liquid culture in the mail so there's that at least to play with once I'm done with these plates. I'm wondering if the point of failure has something to do with my SAB setup. Its pretty crude. And I'm sterilizing a kitchen knife to do the work. On a sterilized counter surface. I read some stuff recently about keeping the surface you work on covered in like a wet towel and have your dishes set on some grate so that you don't disturb spores and dust on the slick countertop surface and get it moving around in the air your working in. I let the SAB sit 10 minutes after I set up the tools and dishes inside too. But then I think I'm just settling a bunch of trich spores on my counter top just to have them flow into my dishes while I'm workin

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Mycolorado
Hobbyist

Registered: 07/23/16
Posts: 8,692
Loc: Interdimensional Bootcamp

Re: AGAR ENVY! (Anything and All things agar!)

[Re: MachineElf226]

1
#29184850 - 04/10/25 07:21 PM (1 year, 5 months ago)

:lolwut:

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Mycolorado
Hobbyist

Registered: 07/23/16
Posts: 8,692
Loc: Interdimensional Bootcamp

Re: AGAR ENVY! (Anything and All things agar!)

[Re: CranberrySteve]
#29184866 - 04/10/25 07:32 PM (1 year, 5 months ago)

CranberrySteve said:

Could I please get an experienced eye over these melmac plates? It's from a vendor LC that was riddled with orange bacteria that I've been cleaning up.

The density and patchy growth bother me. Maybe back the nutes down a tad on your medium to see if it loosens up the culture/coaxes some ropes to get a better isolation of clean growth. As always, take minimal xfers from the leading edge of growth.

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CranberrySteve
Self taught cranberry enthusiast

Registered: 03/06/25
Posts: 82
Last seen: 3 months, 13 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mycolorado]
#29184928 - 04/10/25 09:00 PM (1 year, 5 months ago)

Mycolorado said:

CranberrySteve said:

Could I please get an experienced eye over these melmac plates? It's from a vendor LC that was riddled with orange bacteria that I've been cleaning up.

The density and patchy growth bother me. Maybe back the nutes down a tad on your medium to see if it loosens up the culture/coaxes some ropes to get a better isolation of clean growth. As always, take minimal xfers from the leading edge of growth.

Thanks. Maybe a water plate?

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,112
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: CranberrySteve]

2
#29184938 - 04/10/25 09:20 PM (1 year, 5 months ago)

Sequestering

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