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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Recognizing and dealing with contamination [Re: tree frog] * 1
    #29173006 -

Thanks, this surely is a lot of fun. I'm super excited for the pans. I used to go out and pick them for my dad when I was a kid. I never took them, but I have a vivid memory of my dad having a conversation with the wind screen wipers on his car after he did.


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Seriously, ask me anything about cranberries.

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Re: Recognizing and dealing with contamination [Re: CranberrySteve] * 3
    #29173687 -



My "pin mold" is contaminated with pseudomonas... on purpose. :laugh2:

Rhizopus is fun. So is pseudomonas.


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Question everything, it's very responsive.

Culture Storage

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Re: Recognizing and dealing with contamination [Re: Nichrome] * 1
    #29173713 -

Nice!

I was reading a paper on in vitro oyster/pseudo companionship recently.

Planning to 'contaminate' some colonized grain jars at spawn with azospirillum.  As they produce the same hormone.

I'm already adding them to my casings and I think it's going to be a winner.  With earlier being more win lol


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Recognizing and dealing with contamination [Re: tree frog] * 1
    #29174020 -

That was a good read thanks.


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Question everything, it's very responsive.

Culture Storage

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Re: Recognizing and dealing with contamination [Re: Kizzle] * 1
    #29180719 -

I’m back with more. This is millet+hemp, looked completely colonized with pan cyan. I placed a thin layer of pasteurized peat/verm casing. I have this 48 h later:



It’s too fluffy for typical overlay. It’s about 1 cm deep.  And grew very fast!  Compost it or see what happens?

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Re: Recognizing and dealing with contamination [Re: Imatryin] * 1
    #29180726 -

That's all Rhizopus (pin mold).


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Question everything, it's very responsive.

Culture Storage

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Re: Recognizing and dealing with contamination [Re: Imatryin]
    #29180732 -

Imatryin said:




saved for pin mold example

:awetongue:


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Recognizing and dealing with contamination [Re: mushboy] * 2
    #29181614 -



Rhizopus (pin mold)


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Question everything, it's very responsive.

Culture Storage

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Re: Recognizing and dealing with contamination [Re: Nichrome] * 1
    #29181646 -

Whatever u do don't bust out that Mortierella


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∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Recognizing and dealing with contamination [Re: Nichrome] * 1
    #29181730 -

aptly named


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Learn it, know it, live it


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Re: Recognizing and dealing with contamination [Re: Mackload]
    #29182749 -

So I'm fairly new to this, only completing 1 grow, but I went all in and had 13 tubs, all S2B, and over the past week I've thrown out 10 of them due to contam. After going through my notes, I noticed that they were all spawned with either feed corn or feed milo. The only tubs that I've taken a harvest off, were spawned with brown rice.
This confused me as I have a 15psi pressure cooker that I have added extra weight too so it is truly over 15psi, and my bags are never over 1 1/2 pounds, plus I always sterilize the grain bags for 2 1/2 to 3 hours.
After doing some reading, I found that people have a lot of contam issues with feed grains, and after watching a video from that myco philia guy, I have concluded that feed grade grain is causing all my problems, as no amount of pressure cooking can kill everything.
This may be different depending on where you are in the world. I've seen plenty of successful grows on feed grain, but at least from where I am - our feed grain sucks for mycology.

I'm just mentioning this in case another newcomer is having trouble with contamination. Try brown rice before you throw in the towel, and if you think brown rice is too expensive, I just spent $50 on corn and milo, not to mention the time, coir and space I've wasted. I would have happily spent twice or three times that amount on grain that wasn't fucky


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Seriously, ask me anything about cranberries.

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Re: Recognizing and dealing with contamination [Re: CranberrySteve]
    #29182829 -

I run feed store rye and wheat and have very minimal issues. Proper grain prep is crucial.


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Check out my My Trade List  :opensign:

Check out my wanted Strain List  :opensign:



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Re: Recognizing and dealing with contamination [Re: fungitobewith]
    #29182867 -

There is nothing wrong with feed grain.

It's the skill level of the user, never the grain.  Properly prepared grain is sterile, period.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Recognizing and dealing with contamination [Re: Mr Piggy]
    #29182881 -

Mr Piggy said:
There is nothing wrong with feed grain.

It's the skill level of the user, never the grain.  Properly prepared grain is sterile, period.


Could be. I did follow multiple teks on preparing corn and milo. NSNS, soak overnight, soak and simmer. All contaminated. We'll see how my new rice bags go.


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Seriously, ask me anything about cranberries.

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Re: Recognizing and dealing with contamination [Re: Mr Piggy] * 2
    #29182884 -

Mr Piggy said:
There is nothing wrong with feed grain.

It's the skill level of the user, never the grain.  Properly prepared grain is sterile, period.


Feed grain issues... :laugh2:


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Check out my My Trade List  :opensign:

Check out my wanted Strain List  :opensign:



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Re: Recognizing and dealing with contamination [Re: fungitobewith] * 1
    #29182938 -

Is that ergot


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Recognizing and dealing with contamination [Re: Smellyhobbit] * 1
    #29182960 -

Since you readily admit how new you are to this, have you considered that maybe your Agar game needs work? I hope you aren't shooting spores to grain.

Another possibility is the seals on your jars or bags could be in question. Have you modded your lids at all?

Let's walk it back further... What exactly is your step by step up until you see contams?


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #29182965 -

Sure is. Beautiful little 🐓 spurs. I dig it.🥰

I rarely find them so whole, they're usually somewhat broken for me. I'm a little jelly. 🤣

I just toss them in with the rest of the rye, they colonized just fine. 😎 Granted, I don't get that many but, if I did, I doubt it'd make a difference. Haha


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Chock full o' nuts. ☕

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Re: Recognizing and dealing with contamination [Re: DV8DUG]
    #29182973 -

DV8DUG said:
Since you readily admit how new you are to this, have you considered that maybe your Agar game needs work? I hope you aren't shooting spores to grain.

Another possibility is the seals on your jars or bags could be in question. Have you modded your lids at all?

Let's walk it back further... What exactly is your step by step up until you see contams?


I didn't notice anything on the agar. I went LC - Agar - LC - Grain.

I use bags for grain. I use a fresh syringe and needle every session, and use an induction coil to sterilize the needle in between bags. I even use my SAB for injecting the bags.

I don't think there's anything wrong with my grain prep. As I said, 2 1/2 to 3 hours at 15psi and I've tried multiple teks for hydrating. My feed grain is dirty and I don't know how old it is. I had to kill all the mites in the corn using my oven, and the milo has rocks, dust, stems, and the occasional beetle.
Myco philia had the same issue, I honestly just think the quality of feed grain where I am is shit. I can't ignore the fact that every tub with feed grain contamed and only the rice survived.


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Seriously, ask me anything about cranberries.

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Re: Recognizing and dealing with contamination [Re: CranberrySteve]
    #29182990 -

How long did you soak and how big are the bags? Going over 3 hours might be worth a try.

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Shop: North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale

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