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Re: Ask Quick Questions, Get Quick Answers [Re: the.alchemist] * 1
    #29179836 -

the.alchemist said:



Found this spot on one of my cakes, removed it from the container - Quick Question are the rest of my cakes fucked?



Maybe but it's not a for sure. That could very well be an isolated incident.


--------------------
Question everything, it's very responsive.

Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29179839 -





Maybe but it's not a for sure. That could very well be an isolated incident.



Thank you very much for the feedback!


--------------------
Our Collective Being, The Divine Artist - the Creator God, Aching to express, to exist, Exploded itself/ourself into a cascade of light selves. A cosmic orgasm, Big-banged us into creation,Perfect in ratios, rhythms, and forms. The universe is a storm of light emerging. - Alex Grey

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Re: Ask Quick Questions, Get Quick Answers [Re: neuralnautwork] * 3
    #29179887 -

neuralnautwork said:
Yeah.. this is my first real grow and I've been finding a LOT of things to do differently the next time around :facepalm:

Do you think I'll get a normal flush out of this? or is the poor thing doomed


I think you will get some shrooms, which is more than I got my first try.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: maplepine]
    #29179928 -

maplepine said:
LewDoja said:
With a new blade, and you don't cut it on a cutting board.

Holding it, the new blade can easily slice through the cotton. An old blade will just tear it but still effective.


Interesting.  Could you not also pre-make some little cotton puffs, wrap and sterilize them, and then collect spores and inoculate with tweezers so you don't have to do any cutting at that stage?



The best part about this hobby ..... is there's a million ways to do it, once you've got the basics and a solid aseptic technique - the world's your OYSTER :mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29179963 -

I went to go shake 2 SNAPE jars for the second time and I noticed this fuzzy looking mycelium. I'm guessing it is contaminated? If so, what contaminant? This jar grew a lot slower than the other jar.



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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29179989 -

When do I chuck my jars and start over? I still have no mycelium growth whatsoever after 17 days, I won't chuck them just now but I want to know how long is too long because I'd need the jars to try again. I was thinking 4 weeks before I throw em out judging from what I've read, or should I perhaps wait longer?

They're pan cyan TTBVI and I used Shea25's pans without a pc guide.


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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29179991 -

LewDoja said:
maplepine said:
LewDoja said:
With a new blade, and you don't cut it on a cutting board.

Holding it, the new blade can easily slice through the cotton. An old blade will just tear it but still effective.


Interesting.  Could you not also pre-make some little cotton puffs, wrap and sterilize them, and then collect spores and inoculate with tweezers so you don't have to do any cutting at that stage?



The best part about this hobby ..... is there's a million ways to do it, once you've got the basics and a solid aseptic technique - the world's your OYSTER :mushroom2:


little cotton puff tek! :yeehaw:


--------------------
Learn it, know it, live it


Edited by Mackload (04/06/25 07:39 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: SkanskKorv]
    #29180104 -

SkanskKorv said:
When do I chuck my jars and start over? I still have no mycelium growth whatsoever after 17 days, I won't chuck them just now but I want to know how long is too long because I'd need the jars to try again. I was thinking 4 weeks before I throw em out judging from what I've read, or should I perhaps wait longer?

They're pan cyan TTBVI and I used Shea25's pans without a pc guide.


Was it a spore syringe? Never heard of pans without a pressure cooker, and Pans are also harder than cubensis. Are these grain jars or cakes?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29180121 -

Huskies said:
SkanskKorv said:
When do I chuck my jars and start over? I still have no mycelium growth whatsoever after 17 days, I won't chuck them just now but I want to know how long is too long because I'd need the jars to try again. I was thinking 4 weeks before I throw em out judging from what I've read, or should I perhaps wait longer?

They're pan cyan TTBVI and I used Shea25's pans without a pc guide.


Was it a spore syringe? Never heard of pans without a pressure cooker, and Pans are also harder than cubensis. Are these grain jars or cakes?


Yes it's an ms syringe made from a spore print. People have told me they're not much harder than cubes and that's why I gave them a go.

This is the pans without a pc guide and it seems to work which is why I tried it now while I'm still limited to the pf tek.

https://www.shroomery.org/forums/showflat.php/Number/12165374#12165374

I'm ordering a pc tonight though so if these fail I'll take a more proper route next time. I found Trichoderma in one of the jars but that's the only one I've seen any signs of it in and no other contamination which I can see.


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Re: Ask Quick Questions, Get Quick Answers [Re: SkanskKorv]
    #29180129 -

SkanskKorv said:
When do I chuck my jars and start over? I still have no mycelium growth whatsoever after 17 days, I won't chuck them just now but I want to know how long is too long because I'd need the jars to try again. I was thinking 4 weeks before I throw em out judging from what I've read, or should I perhaps wait longer?

They're pan cyan TTBVI and I used Shea25's pans without a pc guide.



I have TTBVI on grains right now fully colonized ready to S2B. It took around 2 weeks to fully colonized. 17 days with no growth is too long. I would start over. I saw growth within the first day or 2. There is for sure something wrong with your culture or grains. Pretty sure it is the culture. Did you use LC or agar? I used agar. I've tried making LC before but that's harder in my experience. I had no growth using LC before. With agar you can see if the culture is clean or not.

Oh and I just noticed you said you didn't PC the grains? I tried that many times (broke boi tek) when I first started and it was always a fail. Once I got a PC (I bought the cheapest one) I finally started getting successful grows. All of the pictures in my signature are from my first ever grows.

I haven't grown pan cyan yet, I am still new to it. But it is more complicated than growing cubes imo.

You need clean culture and make sure to sterilize your grains properly with the right moisture content in your grains. That is one of the keys to success!

Hopefully someone experienced with growing TTBVI can chime in because I'd like to hear some advice too lol


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Edited by Jrey16 (04/06/25 11:40 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29180150 -

Jrey16 said:
SkanskKorv said:
When do I chuck my jars and start over? I still have no mycelium growth whatsoever after 17 days, I won't chuck them just now but I want to know how long is too long because I'd need the jars to try again. I was thinking 4 weeks before I throw em out judging from what I've read, or should I perhaps wait longer?

They're pan cyan TTBVI and I used Shea25's pans without a pc guide.



I have TTBVI on grains right now fully colonized ready to S2B. It took around 2 weeks to fully colonized. 17 days with no growth is too long. I would start over. I saw growth within the first day or 2. There is for sure something wrong with your culture or grains. Pretty sure it is the culture. Did you use LC or agar? I used agar. I've tried making LC before but that's harder in my experience. With agar you can see if the culture is clean or not.

Oh and I just noticed you said you didn't PC the grains? I tried that many times (broke boi tek) when I first started and it was always a fail. Once I got a PC (I bought the cheapest one) I finally started getting successful grows. All of the pictures in my signature are from my first ever grows.

I haven't grown pan cyan yet, I am still new to it. But it is more complicated than growing cubes imo. You need clean culture and make sure to sterilize your grains properly with the right moisture content in your grains. That is the key to success!



No agar or liquid culture, it's an ms syringe so I expected to to take a week or so to show any signs but even for spores this seems like an awful lot of time.

And I didn't use grain spawn, as I mentioned previously I made a modified pf tek with 1:1:1:1 manure, verm, brf and water bit I suspect it's a bit too wet.

I made an LC from the TTBVI spores a couple of days ago though so I'll be trying that but it feels like a big risk, I just don't feel experienced enough for agar and feel a tad confused over it. I guess it's time to learn though... Is there any simple guide for it?


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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29180155 -

I'm sure that's just a reflection

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Re: Ask Quick Questions, Get Quick Answers [Re: SkanskKorv]
    #29180158 -

Oh ok, I have no experience with MS syringes.

And yea my bad, I didn't read your other post. And I just looked up the Shea25 tek and noticed it is PF tek which I don't have experience with also.

I felt the same way about agar at first but it is a lot easier in my experience. I don't have a link for an agar guide but you can ask here:

https://www.shroomery.org/forums/showflat.php/Number/25662166/vc/1/nt/1437

Or someone else can chime in here.


--------------------


Edited by Jrey16 (04/06/25 11:57 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: SkanskKorv]
    #29180162 -

SkanskKorv said:
When do I chuck my jars and start over? I still have no mycelium growth whatsoever after 17 days, I won't chuck them just now but I want to know how long is too long because I'd need the jars to try again. I was thinking 4 weeks before I throw em out judging from what I've read, or should I perhaps wait longer?




I had 8 quart jars that I threw some LC in that had been sitting on the shelf for a year. Looked clean as a whistle so I didn't bother waiting for my LC test plate to grow out. It grew absolutely nothing. I didn't toss the jars, they were all clean. I reinoculated them with a different LC and they are growing nicely now

If you have no contamination in the jars, I wouldn't chuck any of them. They're still sterile and have the appropriate propagation media in them. No reason to not use them

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29180166 -

just noticed the edges of the substrate pulling away from the side of the tub, is this a sign it's to dry? if so is that the reason my pins seem to be aborting?

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Re: Ask Quick Questions, Get Quick Answers [Re: SkanskKorv]
    #29180170 -

SkanskKorv said:
Jrey16 said:
SkanskKorv said:
When do I chuck my jars and start over? I still have no mycelium growth whatsoever after 17 days, I won't chuck them just now but I want to know how long is too long because I'd need the jars to try again. I was thinking 4 weeks before I throw em out judging from what I've read, or should I perhaps wait longer?

They're pan cyan TTBVI and I used Shea25's pans without a pc guide.



I have TTBVI on grains right now fully colonized ready to S2B. It took around 2 weeks to fully colonized. 17 days with no growth is too long. I would start over. I saw growth within the first day or 2. There is for sure something wrong with your culture or grains. Pretty sure it is the culture. Did you use LC or agar? I used agar. I've tried making LC before but that's harder in my experience. With agar you can see if the culture is clean or not.

Oh and I just noticed you said you didn't PC the grains? I tried that many times (broke boi tek) when I first started and it was always a fail. Once I got a PC (I bought the cheapest one) I finally started getting successful grows. All of the pictures in my signature are from my first ever grows.

I haven't grown pan cyan yet, I am still new to it. But it is more complicated than growing cubes imo. You need clean culture and make sure to sterilize your grains properly with the right moisture content in your grains. That is the key to success!



No agar or liquid culture, it's an ms syringe so I expected to to take a week or so to show any signs but even for spores this seems like an awful lot of time.

And I didn't use grain spawn, as I mentioned previously I made a modified pf tek with 1:1:1:1 manure, verm, brf and water bit I suspect it's a bit too wet.

I made an LC from the TTBVI spores a couple of days ago though so I'll be trying that but it feels like a big risk, I just don't feel experienced enough for agar and feel a tad confused over it. I guess it's time to learn though... Is there any simple guide for it?


What pressure cooker did you get? My sig has a pretty simple and cheap way of doing it in an instant pot, but that is for pouring. 1000ml of gnocchi water gets me around 80 sauce container plates and I do it in a SAB with absolutely minimal contaminations on the agar itself.

A even safer method would oldschool pastyplates, which you could also do in an instant pot or small pressure cooker.

spores to LC is not going to give you clean LC, so scrap that idea. LC is hard to make perfect, even more so from a beginner.

You are also using a 15 year old tek, things change, and a lot of old stuff has become outdated as the shared knowledge increases. Doesn't mean it can't work, but it means it's less reliably going to do so.

For Pan Cyans, you should try this. There is also a huge thread for them specifically here

Your best bet would be use spores to get clean growth on agar, make 1 or 2 transfer from good mycelium until you have good even growth, then use that to inoculate all in one jars with wedges or even by making a LI with PCed water,

You could maybe even re-sterilize the cakes you have now since there is no growth in them. Maybe someone else has a better answer in regards to if that will still work.

edit: you still might see growth from these cakes. If nothing is showing up, at least there isn't mold or bacteria, so in theory your syringe could've been sufficiently clean for pf cakes.

Leave them be, be patient. But I do recommend the links I gave you.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Edited by Huskies (04/06/25 12:15 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Shroom_Ed65]
    #29180172 -

Shroom_Ed65 said:
just noticed the edges of the substrate pulling away from the side of the tub, is this a sign it's to dry? if so is that the reason my pins seem to be aborting?




This is a sign of the mycelium in your tub using the available water in the coir to grow, also your fruits are ~90% water by weight so the more it fruits, the more the coir block will shrink

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Re: Ask Quick Questions, Get Quick Answers [Re: tholos]
    #29180176 -

do you suggest I pour a little water down the sides to moisten it back up?

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29180178 -

I am in SE Asia and I ordered a non branded PC from China and it worked ok. It only went up to 8-11 PSI though. Idk exactly what PSI because the pressure gauge arrived broken lol. And I had to keep an eye on the PC the whole time it was cooking. But once I bought that PC i finally started getting success. I recently bought a presto PC and it's so much better lol, I dont have to keep an eye on it the whole time and don't have to PC for as long.

And thanks for the links!. Im gonna read up


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Re: Ask Quick Questions, Get Quick Answers [Re: Shroom_Ed65]
    #29180179 -

Shroom_Ed65 said:
do you suggest I pour a little water down the sides to moisten it back up?


No, this is the first flush? Condensation on the walls looks right, do you see tiny droplets of condensation on the substrate itself? If not, give it a nice misting, don't let it pool, it should look like raindrops on a spider web.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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