maplepine said:

DERRAYLD said:

That's bacterial so either you aren't sterilizing your loop fully or your water is not sterile.

Those are some options, generally speaking satellite molds are more attributed to our own technique issues.

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Phaethon said:

You mention water used to spread...but nothing about preparing it. Did it go in the IP along with the agar media?

In any case, you can skip the water and remove a possible contamination vector. Just cool the loop in the receiving plate (no need for a separate cooling dish either), pick up some spores with the  agar-wet loop, and streak on the plate. A sterile swab works well for this too - it's more wasteful but also a little less fussy.

Even if the print is dirty, avoiding adding water may give any mycelium that germinates a better chance of outrunning present bacteria long enough for you to take a clean transfer.

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Thanks so much, this makes a lot of sense. I boiled the syringe in water and sucked up some of the boil water, as I saw in a video. However, I also stored it in a ziplock in the fridge, so even though I heated the needle to glowing, there's enough doubt in the rest of the process to point the finger.

I'll look for sterile swabs. I want to avoid ordering things like lab equipment but that should be safe.

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ragamuffin said:

Send it

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BeefSupremeJr said:

this goes out to roscoe, nich, and the other medicinal and gourmet people:

i want to grow chaga.  i forage it from my place in maine but i dont feel good about that.  really.

ive never heard of anyone successfully growing but id like to try.  if anyone has any ideas, dm me.  i have an infinite supply of birch wood/bark.

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The thing about growing chaga is that it parasites a live tree in order to make the medicinal compounds.

You can grow it on other substrates, and it would probably develop some of those compounds. But it's not going to be like wild harvested is my understanding.

Also, because it's a parasite. I wouldn't personally feel bad about harvesting the stone before it tries to fruit and spread to other trees

Huskies said:

What's the best tek for doing cubensis extracts?

I figured it would be a good way to do long term storage and utilizing stuff I wouldn't want to eat as much since the coir is embedded in it. Any experiences or advice?

Also, is there an upper limit for how much mushrooms can be used for a cup of tea and maintain the same ratio?

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GenesisCorrupted said:

Has anyone else been contemplating using Beef’s flamethrower Tek?
Part of me feels like it’s a big old practical Joke way of having you melt your own tub, but part of me really wants to try it.

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GenesisCorrupted said:

Has anyone else been contemplating using Beef’s flamethrower Tek?
Part of me feels like it’s a big old practical Joke way of having you melt your own tub, but part of me really wants to try it.

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san pedro guy said:

Can vacuum sealer be used for bags just for sealing and not vacuuming?

There’s a $20 one local and it’s cheaper than impulse sealers Ive seen

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