Dala said:

Here's something to consider Kloakk...

Tweeq puts the SAB over his kitchen sink for more bottom depth.  Basically, it makes it dub-tub size. Better headroom for bags. I haven't tried it, just sharing tips n tricks.

Here's a good post about it: https://www.shroomery.org/forums/showflat.php/Number/28900600#28900600

Good lucks with whatever you choose

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I read your AIO jar TTBVI journal, and since all my jars are occupied, I did a thing. Don't know how it will turn out doing it in a SAB, but "no guts, no glory" right?

Kloakk said:

Dala said:

Here's something to consider Kloakk...

Tweeq puts the SAB over his kitchen sink for more bottom depth.  Basically, it makes it dub-tub size. Better headroom for bags. I haven't tried it, just sharing tips n tricks.

Here's a good post about it: https://www.shroomery.org/forums/showflat.php/Number/28900600#28900600

Good lucks with whatever you choose

---

I read your AIO jar TTBVI journal, and since all my jars are occupied, I did a thing. Don't know how it will turn out doing it in a SAB, but "no guts, no glory" right?

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I'm digging the clips you're using to keep them sealed.. very pretty colors, also feeling glad i'm not the only one dumping an entire plate per jar/bag, so gangsta

tree frog said:

Some first results from using the azospirillum.



Both of these trays, I inoculated the casing with bacteria.  On my first chloro run, I ran one with grass and one without.  The grass tray pinned a week before the other.  Today, both trays are pinning, but the grass is more heavily (potentially, still the bacteria, it might just be thriving better with the host grass roots).

So, a small data set from early in the run.  But, a promising result so far.

Also, eight days since casing.  Which was quite a bit faster if I remember right.  Different culture though, so the data isn't as good.  Could be a faster culture or a number of other factors could have played a role.

Still, I was expecting like two and three weeks (grass vs. no grass).  So, I'm excited to keep experimenting with the bacteria

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astra said:

I'm digging the clips you're using to keep them sealed.. very pretty colors, also feeling glad i'm not the only one dumping an entire plate per jar/bag, so gangsta

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Thank you. I think they're called "GripStic" bag sealer. Never used them for growing before, but I've used them for food and they hold up good. If I put pressure on the bag, air comes out of the filter and not around the clip, but after inoculating the bags and getting the clips on, I taped the opening of the bags anyway.

And even tho I mostly use 55mm plates I leave 5mm of the outer edge of the plate behind just to be safe. Don't know if it matters in this case tho, I would think doing bags in a SAB is my biggest contamination vector

I was thinking of leaving the bags untouched for a couple of days and then crumble the plate and try to spread it around the bag, any thoughts?

Dala said:

Nice prep work Kloakk! Those look spiffy.

I think it's a good idea to let them colonize a bit before crumbling because you can see if the initial growth looks healthy. If contam starts, you'll probably notice and can manage your expectations. If you mix now, contam might hide on the inside.

Growth will happen fast so you'll be crumbling before you know it.

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Thanks, it went okay for first bags in SAB, but that is an excellent point, I'll leave them be for now and come back with updated pictures in a week or so. I told my self not to get attached to these bags as I know bags and SAB don't mix that well, but we'll see how I feel about it in a week

astra said:

I just came back from Norway this January and was getting stuff from Clas Ohlson, Nille and Ikea...so much cult friendly goodies

I usually dump the whole thing providing they didn't reach the edge but either way is fine as long as you're confident the culture is healthy.

SAB user here so, knocking the jars isn't that bad as long as i don't have more than 7 at a time, my SAB is kinda small but then again i'm a small gal (163cm/49kg).

The only time i'm using bags is for pan substrate and once the PC cycle is over i leave them outside inside a brown paper bag (Amazon style) cooling down overnight, next morning i'd line up my trays after wiping the counter (trays stay with lid on) then i proceed to dump a portion of the substrate followed by the colonized spawn, no SAB... its a very quick and calculated process. Time is of the essence. I use quart jars for my spawn as opposed to those using AIO bags which can be inoculated with LC or an agar wedge. I don't think the way i do cult work is to make things easier, just old and antiquated and very stubborn because i spent the first 4~5 years fighting off LC at all costs. Still using agar to this day.

About whether or not you should wait a couple of days before crumbling the agar for spreading purposes, i never have...i just leave the whole thing take over and when its about 40% and shake them into submission.  That's the thing, i don't really do AIO bags because i'm a lazy slob

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Got it. Yeah this was plates that looked good, but overgrown to the point where I put them in the fridge, not that the edges of the plates look anything but healthy, but ya know, paranoia

Yeah SAB for me as well, and I did a couple of grain jars too, but wanting to try AOI, out of jars and being an "instant gratification monkey" this is where I'm at

Bispo jars are getting there.


163cm ey? Well I'm a whopping 2cm taller

I've read that you sterilize your substrate and spawn in open air a couple of times, and I see you are doing some awesome grows, so I might try doing that along side pasteurization, just to see how that will play out, I guess we all have different environments and I wouldn't know what works best without actually trying it. Appreciate you explaining the process tho, helps me visualize how it should be done, and thanks for confirming what Dala said about leaving the bags alone for now.

Oh and there is nothing slob about the trays you've got there.

Dala said:

Here's something to consider Kloakk...

Tweeq puts the SAB over his kitchen sink for more bottom depth.  Basically, it makes it dub-tub size. Better headroom for bags. I haven't tried it, just sharing tips n tricks.

Here's a good post about it: https://www.shroomery.org/forums/showflat.php/Number/28900600#28900600

Good lucks with whatever you choose

---

Kloakk said:

I read your AIO jar TTBVI journal, and since all my jars are occupied, I did a thing. Don't know how it will turn out doing it in a SAB, but "no guts, no glory" right?

---

Mr Piggy said:

Oh no, your MF looks sick!  It's shedding way too many legos to be healthy.

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Believe it or not, but not a single Lego is from the falcon, but I can't say the same for Upsilon, Tydirium, Droideka or general grievous...





Have kids they said, it'll be fun they said.