Myco816 said:

I had not seen that - thanks! Definitely very similar to what I was planning.

The only big difference is that I was planning to have the inline fan running as an exhaust, rather than blowing into the tub. I was going to filter the air using SFDs on the top of the tub. I can’t tell how your fan is oriented?

---

Even if you have an waterproof fan I think exhausting air out will result in a lot of water/vapor coming out and not be distributed inside the tub, I wouldn't do it and I'm sure Supa knows what he is doing

There is no reason to filter the air, unless you are spawning and moving your tubs in a sterile room all kind of things will be present anyway. I suggest reading the thread and watching the video, even tho I knew I was only going to have this one dreamer tub and using a cloudforge T3 with built in controller I learned a lot reading his thread and watching the video

DERRAYLD said:

20 yr old spores on brf, good progress so far.

---

Simply the best

Kloakk said:

kirkeng said:

Try both

I only ever grown em in a tote so my opinion is biased I’ll always go that route. Dreamers totes definitely will get the job done I’d imagine.

Preference wise I’d say it’s more up to you, getting them to pop well in a tote may take some finesse and extra attention, I’d say moreso how much energy do want to devote?

---

I am pretty invested in this hobby and I enjoy it to the point where I dream about it regularly so I'll do both, thanks

Could anyone take a look at how some of the jars are doing?

---

Those look right to me brother.  Much love for biapo fam.




Planning to spawn when my substrate cools.

tree frog said:

Those look right to me brother.  Much love for biapo fam.




Planning to spawn when my substrate cools.

---

PuffM4gicDr4gon said:

I just ordered all my missing equipment to get my jcm functioning right!

I have 256gph air pump & 16 inch stones, plexiglass, variable computer fan, minute timer, and two sizes of mesh so I can properly sift my manure!

It all will arrive tomorrow, and I'll do my build next weekend.

---

I'm glad you're joining the JCM train, it gets really addicting especially if you were using actual aquariums as fruiting chambers in the early 00's...like going back in time with such nostalgia.

That being said, i don't use plexiglass on mine (my lids are drilled as per the original write-up)  or any type of fan. The air currents remain steady to circulate during evaporation and that is more than enough to keep the tub warm and humid without drying out the surface. You'll know everything is running smoothly if there's condensation all around. I keep my FCs as simple as possible, nothing fancy or WiFi connected and keep an eye on them once a day. Works like a charm.

Kloakk said:

I've read several times here and elsewhere about sterilize vs pasteurize, but I am still a little unsure what to do, I might end up trying both and see how that works out, but would love to hear your thoughts on it.

---

tree frog said:

I don't think that pans care one way or the other about sterilization versus pasteurization.

So, it's really about what you are most comfortable with. Since you already have grain jars, if I was in your position I would use pasteurization.  But if that's not something you're comfortable with, sterilization works too.

Usually if I'm going to sterilize I just put my grains in the substrate and inoculate aio.  But you could also run sterile substrate in a bag and inoculate it like folks do with blocks for gourmets by pouring some grains in the bag in front of a flowhood.

If you are working in a SAB, grain to bags can be a pita.  So that's another thing to consider.

Anyway, either one works. Choose the process that you are most comfortable with and yeah of course running an experiment if you want to see if they behave any differently. In my experience, assuming I didn't fuck up my pasteurization, they don't.

---

Yeah I'm using a SAB, so if I where to do this it would be sterilizing substrate bags in pc, but spawning in open air. I'm hoping pasteurization shouldn't be to difficult, I have a AIO brewing system with both internal(the bottom where the water will be) and external(I can place in the middle of the substrate bag) temperature probe  and just set the system to the appropriate temperate. The temperate would stay within 1-2 degrees celsius of what I set it for.

So with knowing I'll be spawning in open air and using the brewing system for pasteurization, what do you think? I am not trying to see if bisporus cares one way or another, but rather what method I'll have best results with in terms of contamination. I've never used manure or any kind of nutrient based substrate so of course I'm certain I'll get contams the day after spawning

So yeah my main focus is to do what I can, knowing I'll be spawning manure based substrate in open air, to avoid contamination.

tree frog said:

I would pasteurize rather than try open air.  But Pans tend to be quick, and it's possible to get a flush or two before the mold spores take over.  Even if you're letting trich fall on your sterile sub (hence folks getting away with it).

140 freedom units for an hour is enough to kill trich spores.  If the substrate is wrapped in a bag, it will stay good until opened. Once you open it, try to use it soon, as trich may germinate even with the beneficial microbes.  And it can be invisible on substrate without mycelium for it to eat.

An excellent thread on trich put together by Hindsight.  I read this yesterday and learned a lot.

https://www.shroomery.org/forums/showflat.php/Number/28096294

---

Dala said:

Here's something to consider Kloakk...

Tweeq puts the SAB over his kitchen sink for more bottom depth.  Basically, it makes it dub-tub size. Better headroom for bags. I haven't tried it, just sharing tips n tricks.

Here's a good post about it: https://www.shroomery.org/forums/showflat.php/Number/28900600#28900600

Good lucks with whatever you choose

---

Thank you for the tip, appreciate it.

I have a pretty big SAB(150qt), but it is front faced. My bispo jars are only about 12fl oz with around 9fl oz of grain. So I could probably fit my small bags inside the SAB. It will be finicky, but could work with a little practice.


The question tho, will this increase my chance of avoiding contamination before I get to fruiting? I don't want to fruit in bags, I only bought bags for oyster hwfp blocks, and the household agreed that pioppino taste way better and I gro them in monotubs

So when the bags are colonized and I break them up to put in trays and/or mono's are they more resistant to contamination? How are the majority of pan growers doing it? I mean are y'all spawning to sterilized substrate in front of a hood?

Sorry for all the questions I'm just trying to understand it all and find my best path to success with pans

Kloakk said:

Is 140 for an hour(after I reach core temperature) what I should be aiming for? And I'll won't be pasteurizing more substrate then I'll use.

---

Dala said:

I ran my aio jars with grain, bagged poo, and verm. Inoculated with agar in SAB. Once colonized i broke it up and added it to coir in a tray. It worked just like colonized spawn, no worry of contam (that wasn't already present in the spawn).

---

Got it, thanks. I'll do some aio jars next time around. I'll be doing mostly pans and semps for a while, and I guess the best approach is to use the knowledge all of you provide and try it for my self, I might be "cultivating" to much in my head when what I just should do is actually cultivate

tree frog said:

Yup.  And I use the unicorn bags to pasteurize now so I can do some extra, and just leave the bag folded so mold spores don't fall in it.

If I expose the bag to open air, then I want to use it in the next couple of days.  But wrapped on a shelf it should stay stable for quite a while.

---

Bra said:

Panaeolus Bisporus Washington giant. Cloned.

---