Jrey16 said:

GenesisCorrupted said:

People were saying that they were having trouble growing stuff on it. I’m not sure if that was a localized issue or if everyone was experiencing that. I’m still using mine because I got it from before that happened.

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Hmm i live in Philippines and get mine shipped from Thailand. I have noticed I've been having a harder time with agar lately. What if that's the reason 🤔. When you say from before it happened, around when?

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catnip40 said:

you can use the loop end of a sewing needle too

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Good call, I often use fabric pins held with hemostats and find it adaptable and useful. They have those T shaped loop heads. Similar concept as your needle eye tek. 

GenesisCorrupted said:

Jrey16 said:

GenesisCorrupted said:

People were saying that they were having trouble growing stuff on it. I’m not sure if that was a localized issue or if everyone was experiencing that. I’m still using mine because I got it from before that happened.

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Hmm i live in Philippines and get mine shipped from Thailand. I have noticed I've been having a harder time with agar lately. What if that's the reason 🤔. When you say from before it happened, around when?

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I got mine last year around June or July I wanna say. I grabbed like a big old handful from my H Mart.
Later that year, maybe three or four months later. That’s when I first heard people start talking about having trouble growing on telephone agar.

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eilauver said:

Mub said:

Ok I appreciate the reply I will try that. If Myceliun will grow on agar water and not bacteria that eats even the point of adding the nutrients?

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Actually have been wondering the same exact thing. Is there a speed difference, does it stay viable for a shorter time as it runs out of nutrients, what are the drawbacks?

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DV8DUG said:

eilauver said:

Mub said:

Ok I appreciate the reply I will try that. If Mycelium will grow on agar water and not bacteria that eats even the point of adding the nutrients?

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Actually have been wondering the same exact thing. Is there a speed difference, does it stay viable for a shorter time as it runs out of nutrients, what are the drawbacks?

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Running cultures on WA is a time consuming endeavor. It creeps along but you know, you get what you are wanting from the process.

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sk8cracka said:

Almost bright yellow mold/bac on agar(sorry about photo quality) anybody know what this is?  I don't think I have seen simimar

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Kloakk said:

DV8DUG said:

eilauver said:

Mub said:

Ok I appreciate the reply I will try that. If Mycelium will grow on agar water and not bacteria that eats even the point of adding the nutrients?

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Actually have been wondering the same exact thing. Is there a speed difference, does it stay viable for a shorter time as it runs out of nutrients, what are the drawbacks?

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Running cultures on WA is a time consuming endeavor. It creeps along but you know, you get what you are wanting from the process.

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First, that new avatar DV8DUG is fresh.. Second, water agar plates are fine, they do the thing, but bacteria will move along as long as there is moisture, water agar plates should be "dry", meaning 2,5-3% agar.. With that said, cabin sequencing is a better option. With fucked up plates I'll do cabins on my water agar plates.

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CranberrySteve said:

Mr Piggy said:

First of all, that motherfucker better stay the fuck away from my fruit loops.

Second, the point to food coloring in agar is it looks cool.  That's about it. 

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You could switch to flavorless fiber circles, sweetened with civet tears. Now with 25% real beet skins!
It does look cool. I might start adding charcoal just for that reason.

Dang, I wonder if anyone has tried colonizing a jar of moist fruit loops..

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Mkay, now I feel called out.  Actually dried beet skins today for use as dye in pasta

GenesisCorrupted said:

sk8cracka said:

Almost bright yellow mold/bac on agar(sorry about photo quality) anybody know what this is?  I don't think I have seen simimar

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Those pictures are far too blurry.
Can you get much closer?

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Pretty foggy mate.  But I think i got a bit better of one. Literally lions like the yellow posca I use in some labels(not these though)

LewDoja said:

eilauver said:

Mub said:

Ok I appreciate the reply I will try that. If Myceliun will grow on agar water and not bacteria that eats even the point of adding the nutrients?

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Actually have been wondering the same exact thing. Is there a speed difference, does it stay viable for a shorter time as it runs out of nutrients, what are the drawbacks?

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WA has a very thin, barely visible growth.  It's not a viable replacement for normal agar recipes.... it's just another tool at our disposal.

I'd recommend trying .5 % nutes, Also any carb source WITH nutritional yeast- is like booster shot for mycelium.

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callmehippie said:

LewDoja said:

eilauver said:

Mub said:

Ok I appreciate the reply I will try that. If Myceliun will grow on agar water and not bacteria that eats even the point of adding the nutrients?

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Actually have been wondering the same exact thing. Is there a speed difference, does it stay viable for a shorter time as it runs out of nutrients, what are the drawbacks?

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WA has a very thin, barely visible growth.  It's not a viable replacement for normal agar recipes.... it's just another tool at our disposal.

I'd recommend trying .5 % nutes, Also any carb source WITH nutritional yeast- is like booster shot for mycelium.

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How much is .5%? 

Like what's the recipe.  Just using lme and water and agar. 

Im not good at math and don't even know where to start to figure out the actual percentages.

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