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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29170952 -

Telephone brand works great for me

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Re: Ask Quick Questions, Get Quick Answers [Re: oat bag]
    #29170954 -

Same. That’s why I was upset when I heard people were having a hard time using it.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29170974 -

I thought telephone brand set harder than other agar brands. I assume that's kind of the source of why it grows weird sometimes or doesn't germ as well if you are using the 2% agar mix I see most often. I never really had a problem with it when I used agar.


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🌾💮🫘🥖

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Re: Ask Quick Questions, Get Quick Answers [Re: hellofresh] * 1
    #29170986 -

I only use premium gel strength

1000mg/cm2

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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40] * 1
    #29170989 -

catnip40 said:
I only use premium gel strength

1000mg/cm2



I use 980gm/cm2 mr kitty 🐈😸.


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🌾💮🫘🥖

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29171023 -

GenesisCorrupted said:
People were saying that they were having trouble growing stuff on it. I’m not sure if that was a localized issue or if everyone was experiencing that. I’m still using mine because I got it from before that happened.




Hmm i live in Philippines and get mine shipped from Thailand. I have noticed I've been having a harder time with agar lately. What if that's the reason 🤔. When you say from before it happened, around when?


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Edited by Jrey16 (03/28/25 05:16 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29171123 -

Wondering what is going on with my agar cultures!?



They all kind of end up looking like this.  A little all over the place. Looks like it got contaminated after I made a transfer.



This one was inoculated with a loop from a syringe. Over three weeks old. Again, looks like it got contaminated on my last transfer.



This one I just did 3/24. Wondering if that white circle is contam as well?

Sorry about poor pic quality was having trouble getting my phone to focus where I wanted. All of my cultures look a little bit fuzzier than others I have seen here. I’m wondering if that’s something I am doing? Temperatures have been low and probably too much fluctuations could be an issue? Wondering if I should get a new syringe and start over? Might be time for me to get some kind of extra heat source to keep temps more stable? It has been over three weeks now and feeling a little discouraged. I’m pretty sure I know what I did wrong to get the contam, but not sure why all of my cultures look weird.

Any help is appreciated.

Edited by Mub (03/28/25 09:19 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 1
    #29171134 -

My suggestion would be to either take a sterile swab or a sterile cell spreader.

Put a single drop from your syringe in the middle of the plate, and spread it around with either the swab or the spreader.

That should give you a better idea of how clean the syringes, as well as if there is any growth that looks like mycelium instead of another mold or bacteria.

Pasteurizing the syringe is another option. But that's a bit of a work in progress as far as how we do that well.

https://www.shroomery.org/forums/showflat.php/Number/29130723/page/1

And that's not a tech, you need to go through the thread and figure out what is working for people.


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And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29171150 -

Ok that sounds like a good plan. I used all of my strings but will remember that for next time. My first couple of plates had A Lot of contam but the next ones really cleaned up.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub]
    #29171175 -



How slow is hillbilly pumpkin? This pinned before another tub and that tub has already flushed. These don’t even seem close to mature.

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Re: Ask Quick Questions, Get Quick Answers [Re: Breadly710] * 1
    #29171257 -



How do we do PE grows today? spawn: sub ratio? Do we compress? And what about casing, still yes? And is it still pasteurized 50 50+? How do you pasteurize; bucket tek or termometer and a pot? Thank you <3

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub]
    #29171280 -

Maybe you are not wrapping up your plates correctly or hovering over them when doing SAB work.

Transfer these to water Agar.

Also maybe you get contams when pouring plates or perhaps the Agar mix itself is not sterile.


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Please believe in love.

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Re: Ask Quick Questions, Get Quick Answers [Re: Bobbyland]
    #29171349 -

The apartment that I’m moving to won’t fit my 23qtPresto, there is not enough room vertically. I have the induction bottom Presto

I know that Cadco Hotplates are fairly popular but they’re ~$200 new

Looking for suggestions for a portable electric cooktop, can be metal coil based or induction

If you have any pros/cons for one type or the other I’m all ears

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Re: Ask Quick Questions, Get Quick Answers [Re: Bobbyland]
    #29171354 -

Is the mycelium being fuzzy normal?  What is water agar ?

Edited by Mub (03/28/25 12:25 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: tholos] * 3
    #29171356 -

tholos said:
The apartment that I’m moving to won’t fit my 23qtPresto, there is not enough room vertically. I have the induction bottom Presto

I know that Cadco Hotplates are fairly popular but they’re ~$200 new

Looking for suggestions for a portable electric cooktop, can be metal coil based or induction

If you have any pros/cons for one type or the other I’m all ears



I've had this for 6 years, very happy with it, no problems getting my presto up to temp: https://a.co/d/iVLhhga

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub]
    #29171406 -

Mub said:
Is the mycelium being fuzzy normal?  What is water agar ?



Yup fuzzy myc is in most cases just regular mycelium if you work with clean stuff, though I suggest you post pics every time you are unsure.

Water Agar is just water and Agar, use the same recipe you used but don't pour the nutrients in the mix. Mycelium will grow on it and bacteria won't. I'm not sure if molds will spread as well but many ppl use water Agar with no issues.
Make a couple of plates and transfer a wedge or streak the plate using an inoculation loop. Or just use Josex's biopsy method(just search the forum you will find it quickly, I'm not sure if sharing links is allowed) this method is the best if you can spot a patch of clean mycelium. Good luck


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Please believe in love.

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Re: Ask Quick Questions, Get Quick Answers [Re: psilygoose]
    #29171407 -

And it has a timer!?! It seems almost too good to be true. Looks like it might finally be time to replace my old warped presto with an induction 23.

Thanks for the recommendation, psilygoose.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: tholos]
    #29171419 -

tholos said:
The apartment that I’m moving to won’t fit my 23qtPresto, there is not enough room vertically. I have the induction bottom Presto

I know that Cadco Hotplates are fairly popular but they’re ~$200 new

Looking for suggestions for a portable electric cooktop, can be metal coil based or induction

If you have any pros/cons for one type or the other I’m all ears



I use a fairly cheap(60usd) 2000w induction hob, the reason I got the one I did was a timer and wattage/temperature adjustment. After I reached 16-17 psi(13 grams on pressure regulator) I turn the hob to 600watt and set the timer. I don't have to babysit it.

I am on a 240v grid.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Bobbyland] * 1
    #29171442 -

Ok I appreciate the reply I will try that. If Myceliun will grow on agar water and not bacteria that eats even the point of adding the nutrients?

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 1
    #29171460 -

Mub said:
Ok I appreciate the reply I will try that. If Myceliun will grow on agar water and not bacteria that eats even the point of adding the nutrients?



You need nutrients to grow it, but if there are none then mycelium will use the nutrients it has to expand and search for more food until its storage runs out. If I'm wrong someone else will give you a better explanation.


--------------------
Please believe in love.

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