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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Scemilope]
    #29169730 -

regular dawn dish soap can be used but I think it’s different than tween in that one is polar and one isn’t. For a quick serial dilution I think dawn is fine. For a stored syringe I think tween is the shot AFAIK. Wish I could remember where I read this bullshit haha :justdontknow:

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Re: Ask Quick Questions, Get Quick Answers [Re: the_chosen_one]
    #29169732 -

Not for syringe, but ever try printing open air? Might be surprised :heart:

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Re: Ask Quick Questions, Get Quick Answers [Re: phytophthora]
    #29169738 -

Nevermind the polar, non polar thing. Dawn is amphiphilic and tween is too. Wtf?

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #29169748 -

Cool! Thanks! I've seen on some guides to put three large wedges of mycelium agar into a jar and three jars into your substrate. Does that seem pretty accurate?

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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns] * 1
    #29169870 -

Lol groovy. Thats really all I was asking. If a ziplock is necessary or not.


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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29169916 -

I was shaking a colonizing jar and it broke and shattered all over the place. A bunch of colonized grains got stuck inside the front of the FFU. I didn't get a picture of it but I had to get all of the grains out using a toothpick. My question is if the front of the FFU is still good? I was thinking since colonizing grains went inside that it can cause contamination, especially over time. Idk if this was the right thing for me to do but I Lysoled the fuck out the the front of the FFU and I'm gonna leave the FFU running the whole day to hopefully clean things up.
What do yall think?




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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29169925 -

Jrey16 said:
I was shaking a colonizing jar and it broke and shattered all over the place. A bunch of colonized grains got stuck inside the front of the FFU. I didn't get a picture of it but I had to get all of the grains out using a toothpick. My question is if the front of the FFU is still good? I was thinking since colonizing grains went inside that it can cause contamination, especially over time. Idk if this was the right thing for me to do but I Lysoled the fuck out the the front of the FFU and I'm gonna leave the FFU running the whole day to hopefully clean things up.
What do yall think?





That is...not ideal. The proper equipment to test is pretty expensive. That said I'd bet it still works pretty well. Maybe leave a few agar plates open in front of it while it is running for a couple minutes. In a few days that will give you some indication of how it's working. Let it fully dry first.

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Re: Ask Quick Questions, Get Quick Answers [Re: psilygoose]
    #29169950 -

psilygoose said:
Jrey16 said:
I was shaking a colonizing jar and it broke and shattered all over the place. A bunch of colonized grains got stuck inside the front of the FFU. I didn't get a picture of it but I had to get all of the grains out using a toothpick. My question is if the front of the FFU is still good? I was thinking since colonizing grains went inside that it can cause contamination, especially over time. Idk if this was the right thing for me to do but I Lysoled the fuck out the the front of the FFU and I'm gonna leave the FFU running the whole day to hopefully clean things up.
What do yall think?





That is...not ideal. The proper equipment to test is pretty expensive. That said I'd bet it still works pretty well. Maybe leave a few agar plates open in front of it while it is running for a couple minutes. In a few days that will give you some indication of how it's working. Let it fully dry first.


I'm hoping it's still good..

Anyone else have an opinion or advice?


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29169991 -

I'm sure it's just fine. Especially since the mechanics didn't get wet or whatever. As long as the "flow" isn't obstructed, all good.

Any "grain juice" 🧃 will be dried out and continue to be...I don't see how any contaminants could take hold or enjoy that. Haha

I'd be more concerned with Lysol fumes in my face. 😷🤣

All jokes aside, I wouldn't stress too much about it but perhaps others with FFU's will chime in. I'm just in a jesting mood. 🃏


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Chock full o' nuts. ☕

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Re: Ask Quick Questions, Get Quick Answers [Re: ShitdickNancy]
    #29169992 -

Nice to hear. Thank you. Yea im hoping the smell of lysol would go away. It should, since I'm leaving the FFU on for a while


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Edited by Jrey16 (03/27/25 12:52 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29170010 -

I honestly think it will be fine if the filter didn’t get punctured. I would run it for a day or two like you mentioned. Something else you could do if the grain was concentrated right in the area that you work in front of; rotate the filter 180 degrees. Not front to back, but like a clock face. That way you move the affected surface area away from the work area as much as possible. In time any surviving microorganisms will be dried out and blown away the more you use it.


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Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
    #29170019 -

ReverendMyc said:
I honestly think it will be fine if the filter didn’t get punctured. I would run it for a day or two like you mentioned. Something else you could do if the grain was concentrated right in the area that you work in front of; rotate the filter 180 degrees. Not front to back, but like a clock face. That way you move the affected surface area away from the work area as much as possible. In time any surviving microorganisms will be dried out and blown away the more you use it.


Ahh good idea but i wouldn't be able to rotate it because the person who put it together made it so I can't take it apart to rotate it. I'll running the FFU until tomorrow, and I'll also be sending agar to grains tomorrow. Let's see what happens..


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29170025 -

you may want to do yourself a favor and get a hypochlorous acid generator for future cleaning.  you can get cheap little shitty USB ones or something fancier depending on the volume of HOCl you want to generate. 

HOCl is inexpensive, safer, quite effective, and cuts a lot of rinsing out of the equation.

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Re: Ask Quick Questions, Get Quick Answers [Re: dinosaurs]
    #29170033 -

dinosaurs said:
you may want to do yourself a favor and get a hypochlorous acid generator for future cleaning.  you can get cheap little shitty USB ones or something fancier depending on the volume of HOCl you want to generate. 

HOCl is inexpensive, safer, quite effective, and cuts a lot of rinsing out of the equation.


Never heard of that. I gotta look it up


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 1
    #29170083 -

Jrey16 said:
I was shaking a colonizing jar and it broke and shattered all over the place. A bunch of colonized grains got stuck inside the front of the FFU. I didn't get a picture of it but I had to get all of the grains out using a toothpick. My question is if the front of the FFU is still good? I was thinking since colonizing grains went inside that it can cause contamination, especially over time. Idk if this was the right thing for me to do but I Lysoled the fuck out the the front of the FFU and I'm gonna leave the FFU running the whole day to hopefully clean things up.
What do yall think?





A couple of decades ago the common wisdom was for IV techs to squirt any left over meds into or on to the filter face while working in a flow hood. Terrible idea, which we worked hard to get them to stop doing. Having said that I have tested plenty of filters that were all sorts of different colors from dried stuff stuck to them. They still passed leak test. My biggest concern would be nutrient from the grain remaining on the filter and growing something that subsequently would get blown on to your work. The best defense for this would be to keep whatever got on there as dry as possible. I really think you’ll have no troubles.


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Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29170117 -

The grains are just going to dry out. You're fine.

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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers]
    #29170120 -

Are there issues using agar plates that have significant pins, or do they all just turn to mycelium in the jar?

Things are even more erect. Its already a plin clone so IDK about more cloning (APE)



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I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers]
    #29170130 -

Yup I'm leaving the FFU on for a while too. Thank you guys! Ngl, i was kinda freaking out earlier 😂


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29170141 -

I'll be sending agar to grains tomorrow and make a few transfers.. let's see what happens


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29170142 -

Okay... I don't really have the free time to read through the 16k of posts in Woodlovers thread. Can someone inform me of the best spawn medium for Ps. Allenii? I have never grown a wood lover before and I have full plates all of a sudden.


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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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