Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Substrate Mix   Myyco.com APE Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order

Jump to first unread post Pages: < First | | 2637 | 2638 | 2639 | 2640 | 2641 | 2642 | 2643 | 2644 | 2645 | 2646 | 2647 | 2648 | 2649 | 2650 | 2651 | 2652 | 2653 | 2654 | 2655 | 2656 | 2657 | | Last >
Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29166136 -

callmehippie said:
Quick question.

If you received a wedge in the mail from a cleaned up plate..

Do you treat this wedge like a normal transfer or do you treat it like a germ plate and streak it?

Usually the wedges I receive are very very smashed and streaking is kind of the only option. However these wedges I have at the moment are nice and big and intact...



Also I'm planning on making up some agar soon. I've never done it. So I have no experience with pour or no pour.

A buddy of mine gave me this sterilizing canister for 90 mm dishes



Has anybody ever used one of these?
Only problem is it only holds 10 plates.... So.. for no pour other people are also just using a certain size jar with paper towels? basically just like the canister above?

Or in half pint wide mouth as the dish itself?

Does anyone have a picture example of using a  jar and  paper towles like a canister to hold no pour plates for the PC. 

No pour seems like it may be the better route for me to go cuz my hands are tiny and I can barely even hold a stack of five plates at the same time while trying to pour in a sab lol.

But I guess I could just make stacks of 10 and then pull one plate off the stack at a time. pour it. and then make another stack with the ones I just poured. I just have to deal with how much extra time it takes.

All opinions and tips are welcome.


I use those canisters and do no pour in them I can usually fit like 12-15 in them. I have used eisco brand petris viabooth and microppose


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: catnip40] * 2
    #29166148 -

in general its always correct to take a safety transfer from anything new

if its clean and viable its not like you did anything wrong


--------------------
Willpower is the one true virtue


Edited by smalltalk_canceled (03/22/25 07:43 PM)

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29166158 -

It’s definitely more blue/gray than green. I kind of tapped some of the white area around it and it’s very soft like a marshmallow.

When I’ve experienced T before, I feel like it showed up on the surface of the sub first rather than the blobs themselves. That and the fact that this culture has performed pretty well throughout the process and has been colonizing the sub quickly gives me some optimism. That said I will not be surprised if the entire thing is green by tomorrow.



--------------------
The Hitchhiker's Guide

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Myco816] * 5
    #29166316 -

Quote:
myc piss


Not too fond of this association, It's metabolite excretion, not waste like urine.

The term makes people think it's something dirty or bad when, in reality, it’s a natural defense mechanism against contaminants and stress.
It’s mostly an overproduction of organic acids and enzymes in response to stress, substrate conditions, or contamination. So instead of “myc piss,” saying “metabolite buildup” would make more sense, or:

Mycelial Metabolites – Accurate and scientific.

Exudates – General but correct.

Protective Secretions – Describes the function.

Metabolic Byproduct – Neutral and precise.


Can also be overly produced by natural processes, & stress not just contamination....
prolonged metabolism, environmental factors, or nutrient processing.

Aging Mycelium – Older cultures naturally excrete more metabolites as a byproduct of prolonged metabolic activity.

High Nutrient Density – Some substrates (especially over-supplemented ones) can trigger excess metabolite production as the mycelium processes and breaks down compounds.

Moisture Imbalance – If the substrate is too wet, mycelium can excrete more metabolites to help regulate its internal environment.

Extended Colonization Times – If mycelium is left in jars or bags for too long without fruiting, metabolites can build up due to prolonged metabolic activity.

Temperature Stress – Higher incubation temperatures can lead to more metabolite production, possibly as a protective mechanism.

Edited by LewDoja (03/22/25 11:08 PM)

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Myco816] * 2
    #29166345 -

Myco816 said:
I’m going to cut that shit out and see what happens.


Since it's ochra and overlays like a bitch it's still possible to get a harvest.

The bluing and metabolites could be caused by other than contams.

Assess the environmental conditions, but don't go playing doctor with a scalpel unless it's just to remove the overlay from the surface...
and don't toss it untill confirmation of invaders...


Edited by LewDoja (03/22/25 11:34 PM)

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29166354 -

Thanks. It’s temp controlled at 72-74F. The moisture seems good to me. Any other tips re environment?


--------------------
The Hitchhiker's Guide

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Myco816]
    #29166356 -

When you say it’s temperature controlled. You’re not using a heating mat are you?


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29166357 -

No, I have a little grow room in my basement for plants. The basement is otherwise a little cold - the grow room is heated by a small electric furnace (no forced air).


--------------------
The Hitchhiker's Guide

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29166376 -

mycelium doesn't produce urine but metabolites are secondary excretions, making them pretty closely analogous to urine. perfectly healthy mycelium will produce metabolites.  there is not a 1:1 correlation between metabolites and stress or contam. 

interestingly, fungal metabolites are actually being studied for their suspected medicinal properties.  so in the future you might get some medicines made with mycelium potties. :smile:

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: dinosaurs]
    #29166432 -




100 .5 Amazon capsules does anyone else prefer capsules pros/cons


--------------------


Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: gmj]
    #29166434 -

gmj said:



100 .5 Amazon capsules does anyone else prefer capsules pros/cons



What size capsules are those? Been wanting to make capsules that hold around .2-.25 for a while now but been too lazy lol. I got size 1 capsules I think

I never tripped off capsules but I'd use them for microdosing


--------------------


Edited by Jrey16 (03/23/25 01:16 AM)

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: dinosaurs] * 1
    #29166435 -

Quote:
....mycelium potties


:trekfacepalm:


There's already many pharmaceuticals created from fungal metabolites.

Most notable Penicillin.

Lovastatin (Aspergillus terreus) – A statin used to lower cholesterol levels.

Edited by LewDoja (03/23/25 01:28 AM)

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Myco816]
    #29166437 -

Myco816 said:
Thanks. It’s temp controlled at 72-74F. The moisture seems good to me. Any other tips re environment?



How does it smell when you first take off the lid?

Edited by LewDoja (03/23/25 01:18 AM)

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Anonymous]
    #29166439 -

For everybody that pours. When you order Petri dishes do they really come sterilized or is this something you have to worry about and actually sterilize them yourself before use? Or is it a brand thing?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 1
    #29166440 -

Jrey16 said:
gmj said:



100 .5 Amazon capsules does anyone else prefer capsules pros/cons



What size capsules are those? Been wanting to make capsules that hold around .2-.25 for a while now but been too lazy lol. I got size 1 capsules

I never tripped off capsules but I'd use them for microdosing


They are 000 and hold .5 each maybe 00 will hold .2 not sure
I think I like capsules because
I don't have to taste them and the come up is better imo


--------------------


Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 2
    #29166445 -

callmehippie said:
For everybody that pours. When you order Petri dishes do they really come sterilized or is this something you have to worry about and actually sterilize them yourself before use? Or is it a brand thing?



Make sure it says "STERILIZED" in the description or title. And will say how it was (ethylene gas, or gamma radiation)

I also pay attention to the quantity per sleeve I prefer 10.

It has to say "autoclavable" to be able to do it yourself...

Edited by LewDoja (03/23/25 01:32 AM)

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: gmj]
    #29166518 -

I can fit .5 in size 0 and .3 in size 1


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29166527 -

:mushroom2:

there's quite a few more mycelium piss drugs than that, and some very promising ones being researched.


Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29166549 -

I inoculated these jars with agar on the same day almost 2 weeks ago. The front jars are B+ and Stargazer and almost fully colonized. And the jars in the back is SNAPE (Starry Night APE). Is it normal for PE varieties to colonize this slow? Or is there something that might be wrong? I shook them last week or so.



These are the SNAPE plates I inoculated the grain jars with. It took a month or maybe a little more than a month to get to this much growth and they weren't even fully colonized.



--------------------


Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Myco816] * 1
    #29166554 -

Myco816 said:
It’s definitely more blue/gray than green. I kind of tapped some of the white area around it and it’s very soft like a marshmallow.

When I’ve experienced T before, I feel like it showed up on the surface of the sub first rather than the blobs themselves. That and the fact that this culture has performed pretty well throughout the process and has been colonizing the sub quickly gives me some optimism. That said I will not be surprised if the entire thing is green by tomorrow.





I'm going to land on team skeptic with this one and say yeet it into the sun.  The fact it's acting up in multiple spots says to me that it's dirty all the way through.

Now if you'll excuse me I have a moisture imbalance that's about to result in some productive secretions.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

Extras: Filter Print Post Top
Jump to top Pages: < First | | 2637 | 2638 | 2639 | 2640 | 2641 | 2642 | 2643 | 2644 | 2645 | 2646 | 2647 | 2648 | 2649 | 2650 | 2651 | 2652 | 2653 | 2654 | 2655 | 2656 | 2657 | | Last >

Shop: Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Substrate Mix   Myyco.com APE Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* quick question, finished colonizing? usefulidiot 1,861 1 04/06/03 06:31 PM
by kindkesey
* Quick Question grateful_head 1,240 2 08/18/01 03:40 PM
by Billyblastoff
* New to the boards, and a quick question to start ! Anonymous 1,846 4 03/11/02 03:18 AM
by 2shrewmen
* some quick questions about my treasure coasts alf 3,858 19 11/16/01 01:54 AM
by Anonymous
* Quick Question needs quick answer Wheedhead! 1,186 2 01/19/03 09:06 AM
by Wheedhead!
* quick question SuppaFly 1,044 1 09/18/02 04:41 PM
by mickey_rourke
* Re: Quick Question
( 1 2 all )		 Anonymous 4,550 22 06/14/00 05:46 AM
by hitterg
* Quick Question LXTC 1,808 11 09/24/02 10:09 PM
by LXTC

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: DERRAYLD, Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,187,220 topic views. 2 members, 577 guests and 202 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.065 seconds spending 0.039 seconds on 16 queries.