GenesisCorrupted said:

Does anybody remember the old school bubble hash with the multiple bags in buckets of ice?
I wanna get some of those bags, but I’m very skeptical of the quality on Amazon. Does someone have a good recommendation for those types of bags?

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DERRAYLD said:

The Amazon bags are fine, they're just made from a different material but they do the same thing.

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catnip40 said:

ya i do think there is multiple reasons for it and contam is one but i definitely think genetics alone could be the case sometimes too

i notice scalloping and weird agar growth and shit on crosses/isolated mutations ect than regular cubes but i dont know how long ive been paying that close attention or keeping track either

but i do know that ive started to notice this stuff a lot more with unstable crosses/mutations and weird non "regular cubes"

im still a staunch hidden contaminant denier

just put ur shit on PDA it aint gone hide no more

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fahtster said:

From what I’ve heard, monokaryons can display scalloping and I definitely agree that crossed myc can put out some funky ass mycelium.  I think there’s def some factors that come into play as far as the “enmeshed” thing goes.. like the colonizing speed of both species involved.  If you have a slow colonizing mycelium but a fast reproducing bacteria, you’re gonna have a hellava time getting away from it.  Myc can burrow into the agar but the bacteria grows on top.  Just spitballing here

Thems there’s the human component.. I think assuming everyone has the same agar skills/sterile technique is a mistake and overlooked. 

And what about this “benefial bacteria” we hear about all the time?  Could it be that this is what’s riding along with some of the scalloping cultures that’s causing the display it does on agar but then not causing problems once spawned?    who knows?  Ik I’ve seen ppl scope out scalloping cultures and find bacteria. 

Let’s say you have a clone that’s scalloping, if it is genetic, why is some of it showing scallops and some is growing normally?  Is it the same reason we see sectoring in clones? 

I asked that question a while ago and didn’t get any kind of answer.. something along the lines of “where are we on there being multiple genetic lines in clone cultures; have we landed on a definitive answer to that?”  So I’m pretty uninformed on that.  It does seem that we can continue to isolate from clone cultures to reduce phenotypes but wanted something more concrete

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theres some links i can get that talk about sectoring and the genetics but it could just be multiple things too not so much as scalloping but i think scalloping could be a type of sectoring either way

Care and handling of cultures of the cultivated mushroom (Agaricus bisporus)
https://plantpath.psu.edu/about/facilities/mushroom/cultures-spawn/care-handling.pdf


Inheritance of Strain Instability (Sectoring) in the Commercial Button Mushroom, Agaricus bisporus
https://journals.asm.org/doi/pdf/10.1128/aem.60.7.2384-2388.1994



A novel method for inducing the expression of sectors in Agaricus bisporus.
https://www.cabidigitallibrary.org/doi/full/10.5555/19941601254


6. S1 Dikaryons
Chapter 6: Breeding in L. Edodes in Genetics and Breeding of Edible Mushrooms - A.C. Chang pp. 114-115



re: clone genetics
Ksss squat toad spaceship harvest to clone to spawn.

DERRAYLD said:

Well the current trend is actually fresh frozen/ freeze drying and then washing full plants and pressing the hash for rosin.

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Smellyhobbit said:

catnip40 said:

ya i do think there is multiple reasons for it and contam is one but i definitely think genetics alone could be the case sometimes too

i notice scalloping and weird agar growth and shit on crosses/isolated mutations ect than regular cubes but i dont know how long ive been paying that close attention or keeping track either

but i do know that ive started to notice this stuff a lot more with unstable crosses/mutations and weird non "regular cubes"

im still a staunch hidden contaminant denier

just put ur shit on PDA it aint gone hide no more

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I’m also skeptical of “hidden” contamination in that I think it’s far rarer than people are claiming.

Scalloping is a sign of weak genes. Weak genes shit the bed and get eaten by competitors once sent, which is often mistaken as having been there the whole time. I’ve never bothered to sus good genes from scalloped plates, but I’ve definitely sent scalloped plates with mixed success. If it was hidden contam the success rate would be functionally zero, id imagine.

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Yeule said:

Can some kind TCs help me out?

Im kinda on the fence about my jars. They looked pretty good pre-shake a few days ago


But now a few days later im having doubts. Ain't they a bit too fluffy?



Closeups




Thanks everyone:)

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DERRAYLD said:

Well the current trend is actually fresh frozen/ freeze drying and then washing full plants and pressing the hash for rosin.

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LewDoja said:

Here's exactly why some ppl say not to use food coloring (especially green)

Sorry bud but I doubt you were able to Shake you're jar into perfect dispersal of your agar.

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Good job with the rice....what's your tek.


@S.P.G

Quote:

you gotta sterilize the grains before you noc it up

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