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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 1
    #29159785 -

LadysKnight said:
My sab is a permanent fixture. It keeps the air still. I know it's just as good now as when I left it, likely better. If I spray something in there, it just stirs up the air and adds non-sterile particulate that can take days to fully settle. I cleaned it once, last year.



LadyKnight, everytime without fail, you keep opening my eyes again and again. Thanks you man

Also i hear you advocate for no pour glass petri dishes that right?


--------------------
Around my neck, a friendly machine

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Re: Ask Quick Questions, Get Quick Answers [Re: oatsnhoes]
    #29159787 -

oatsnhoes said:
I prepped some coir for a couple of shoe boxes a few months ago in an insulated cooler. The leftover coir has been sitting in the cooler ever since, unopened. Is it still good to use after sitting for a while?



From what i understand, as long as its *just* coco coir and pure minerals like verm it should be ok, give it a sniff tho play around with it to make sure maybe

Also W pfp man i love mag bay


--------------------
Around my neck, a friendly machine

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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40]
    #29159791 -

catnip40 said:
ya i stopped cleaning my sab and waiting to work after awhile

do people even mist their sabs with soap water anymore cuz i abandoned that pretty early on and just used it dry too lol



Wait do you even take your sab apart sometimes? Or does is permanent sit somewhere with the hand holes covered (or not)?
You just go to it, clean the things you put inside, wait, and go at it?

Thats some crazy stuff man imma need more info please


--------------------
Around my neck, a friendly machine

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29159799 -

I tried my first grow bag crop last summer. Two bags colonized well. I did the break and shake. Then, no growth for months.

I then bought nine grow bags from three different suppliers. I used three spore syringes from two different suppliers to inoculate. They are colonizing at widely varying speed.

I’ve opened up two bags for fruiting ten days ago.

One is pinning nicely.

The other is a solid block of white with occasional, tiny sprouts with black heads that can barely be seen in the picture and which may indicate contamination. Especially due to possible contamination, I’m thinking about just getting rid of it.

Any suggestions are appreciated.




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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29159819 -

LewDoja said:
Try revs tek , I've had good results (also modified dehydrator with toggle switch for fan only, no heat)




https://www.shroomery.org/forums/showflat.php/Number/27829033
Quote:
P.S. Don't use a whisk, use a wooden spoon instead. Whisk will cause undesirable bubbles or foam.



Gelatin will give better texture (I used small amount of agar as well)
But you've gotta let it bloom in cold water first



Thanks. Rev's tek is where I started from. I let them dry till quite dry btw. They also shrink and I actually like that AND they appear less apetitely AND they are even smaller. I use 1ml molds.

I guess I just have to switch to a different agar brand. Rev's tek uses about half of my agar content. Switching to gelatine is a good suggestion. Though I don't really like the taste of gelatine, but combined with the citric powder probably negligible. Maybe other agar is also less clumping.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub]
    #29159831 -

Mub said:


Is there anything salvageable in this dish? There’s a spot of orangish/pink there. I had one dish that was very infected with pin molds. Two others that don’t seem to be doing much of anything but maybe a little growth. I used a pre mixed agar that was maybe a little stiff. It just seems to crumble when I put my inoculation loop or spore syringe in it.



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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 1
    #29159851 -

Mub said:
Mub said:


Is there anything salvageable in this dish? There’s a spot of orangish/pink there. I had one dish that was very infected with pin molds. Two others that don’t seem to be doing much of anything but maybe a little growth. I used a pre mixed agar that was maybe a little stiff. It just seems to crumble when I put my inoculation loop or spore syringe in it.





Absolutely, agar is meant for this plate. Transfer the better looking bits far from contams, to new plates.

That premix seems to be working for now, meanwhile order some plain agar powder and LME powder.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 1
    #29159859 -

Oh I’m absolutely not doing the pre mixed agar again lol. I had two other dishes with no signs of growth. Maybe I got the loop too hot or the was just not enough specimen? I guess it’s too hard to say.

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Re: Ask Quick Questions, Get Quick Answers [Re: FireThief] * 2
    #29159868 -

FireThief said:
I tried my first grow bag crop last summer. Two bags colonized well. I did the break and shake. Then, no growth for months.

I then bought nine grow bags from three different suppliers. I used three spore syringes from two different suppliers to inoculate. They are colonizing at widely varying speed.

I’ve opened up two bags for fruiting ten days ago.

One is pinning nicely.

The other is a solid block of white with occasional, tiny sprouts with black heads that can barely be seen in the picture and which may indicate contamination. Especially due to possible contamination, I’m thinking about just getting rid of it.

Any suggestions are appreciated.







Since you went spore to bag, I would not do a traditional spawn. Follow Beef Supreme on: Rescuing Your Fucked-Up All-in-one kit (Or whatever less-than-ideal situation you have)
The pink or any mold is usually a deal breaker. But that method may get you fruits before the end. Meanwhile, read the HHG in your spare time.
Good luck!


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: Yeule] * 4
    #29159877 -

Yeule said:
LadysKnight said:
My sab is a permanent fixture. It keeps the air still. I know it's just as good now as when I left it, likely better. If I spray something in there, it just stirs up the air and adds non-sterile particulate that can take days to fully settle. I cleaned it once, last year.



LadyKnight, everytime without fail, you keep opening my eyes again and again. Thanks you man

Also i hear you advocate for no pour glass petri dishes that right?



I don't like being dependent on the postal system or the supply chain. Also don't like to have "odd" trash, I'm paranoid.
I landed on cheap glass for visibility, reusability and cost. At the time it was the only choice. Now there are autoclavable clear plastic ones. Not cheap, but it's another option.

No pour makes the most sense to me and my methods. After PC is cold, I place it next to the sab, move sterile items into sab, go to work. Nothing to wipe or spray.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 2
    #29159900 -

Imo


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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted] * 1
    #29159942 -

Was thinking the same thing thank you.

When I did this I pit some of the specimen from the spore syringe into an extra petri dishes I sterilized in the instapot. I used the inoculation loop to transfer. Not sure why my plate looks so weird but it was like my agar was just crumbling as I used the loop through it. Am I supposed to just move it across the surface? Or really get in there like I was doing? I tried my best to use the zig zag pattern Bod shows in his agar tek

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 1
    #29159972 -

When I’m making my agar to agar transfer am I just cutting the mycelium and dropping on the new agar or do I need to press it or mix it in?

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 1
    #29159978 -

Just get it on the plate. I actually like making cuts and barley getting them on my scalpel so that the transfer just sticks to the receiving plate

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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40] * 1
    #29159984 -

Thanks catnip

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub]
    #29160047 -

What u guys think? could it be anything else? grown from dried cube spores in agar 2t, way too nute, grew tomentose, i did put it into fruiting



last 2 pics are from yesterday

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork] * 1
    #29160069 -

cybork said:
LewDoja said:
Try revs tek , I've had good results (also modified dehydrator with toggle switch for fan only, no heat)




https://www.shroomery.org/forums/showflat.php/Number/27829033
Quote:
P.S. Don't use a whisk, use a wooden spoon instead. Whisk will cause undesirable bubbles or foam.



Gelatin will give better texture (I used small amount of agar as well)
But you've gotta let it bloom in cold water first



Thanks. Rev's tek is where I started from. I let them dry till quite dry btw. They also shrink and I actually like that AND they appear less apetitely AND they are even smaller. I use 1ml molds.

I guess I just have to switch to a different agar brand. Rev's tek uses about half of my agar content. Switching to gelatine is a good suggestion. Though I don't really like the taste of gelatine, but combined with the citric powder probably negligible. Maybe other agar is also less clumping.



I used two jello flavors and they were so good was difficult not to just keep eating them.. also had the citric acid/sugar mix coating,,,,YUM

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Re: Ask Quick Questions, Get Quick Answers [Re: Ikarus21]
    #29160082 -

Ikarus21 said:
What u guys think? could it be anything else? grown from dried cube spores in agar 2t, way too nute, grew tomentose, i did put it into fruiting



last 2 pics are from yesterday



First off welcome!

So you're saying you scraped spores to agar, which had too much nutrients...how much is too much?

...took 2 transfers (to new plates)

...then you put (what) into fruiting?

But aren't sure that you actually put mushroom myc, or if your substrate wasn't clean??

Based off just the pics, I'd say you have mushroom mycelium growing on coir.

Could it be something else?

Yes there could be something else alongside it*.... doesn't necessarily mean that there is.


Edit:
After looking at your other posts, how did the germination plate turn out from taking gill fragments off a dried specimen?

Like how soon till noticeable growth, was there any contamination?

The grain spawn looked a Lil sus in the last 2 pics and 40% within 3 days seems like almost too fast for a2g, but not impossible.

What was the s2b methods/ratios?


--------------------
A_wise_man said:
"Bad drugs tell you, that you want more;
  Good drugs tell you, that you've had enough"



🎶  KIND TC's - by: Tri  🎶

Edited by LewDoja (03/16/25 07:15 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29160091 -

Does agar need uniform growth to be considered clean? I've been having trouble getting 2 cultures get uniform growth. It has just been a few days since last transfer but will plates like this end up ok if it doesn't mold up or anything? Or should I make another transfer? Is it the genetics? All the other cultures I'm working on have nice uniform growth but these GTs and Mr. Peanut are not





--------------------


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 1
    #29160099 -

Those all look clean to me just sectored growth

I would still probably transfer most of those again though

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Shop: MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Boomr Bag


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