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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Sarnath] * 12
    #29152161 -

Bit of wire, piece of dowel, screwdriver and pliers.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29152165 -

Metal wire it is, then :laugh:


--------------------
You are a prisoner of the road you follow
Only the untrodden snowdrift is free
- A. Hellaakoski

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Re: Ask Quick Questions, Get Quick Answers [Re: Sarnath] * 6
    #29152172 -

Or wire and exacto


That was 3 sabs ago, idk how I used 4" holes


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It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight]
    #29152180 -

Exacto handles work well, I just wanted to MacGyver. I like using the twisted wire because the pressure I put on agar with them is consistent. Paper clips and bread wire ties work but aren’t consistent.

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29152184 -

The Tao said:
Exacto handles work well, I just wanted to MacGyver. I like using the twisted wire because the pressure I put on agar with them is consistent. Paper clips and bread wire ties work but aren’t consistent.


Your handles are definitely better looking. I went with thick old copper coaxial cable. Had to hammer one end flat to use the exacto handle.


--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao] * 4
    #29152187 -

The Tao said:
Exacto handles work well, I just wanted to MacGyver.


MacGyvering stuff is one of the noblest hobbies a person can have.


--------------------
You are a prisoner of the road you follow
Only the untrodden snowdrift is free
- A. Hellaakoski

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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 3
    #29152189 -

Zit popping tools, I just throw them in a sterilizer baggie and bam


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 4
    #29152208 -

Where’s the fun in that?

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 2
    #29152210 -

you can use the loop end of a sewing needle too


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40]
    #29152228 -

Do these look relatively clean to you guys, they're from a dirty plate I'm trying to clean up.About to make another transfer soon.
Bit new to all of this so not even entirely sure it's mushroom mycelium. Also the dark border you can see around the leading edge is transparent and fuzzy is this normal?


Also whilst I'm here this is my first streak plate, made a bit of a mess of the agar with my loop 😅 does the sector on the left look any good to take a transfer??
Thanks in advance for any help you can give

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Re: Ask Quick Questions, Get Quick Answers [Re: Bemused Hippie]
    #29152259 -

Is heavy condensation (pooling water at the bottom of the jar) a clear sign of bacterial contam?
My jars do hardly have any condensation until full colonization.
If i let the jars rest for 1 or 2 more weeks, the myc gets more and more dense and the condensation increases until there is pooling water at the bottom. Is it possible that this is only due to the temperature difference inside and outside the jar?

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Re: Ask Quick Questions, Get Quick Answers [Re: Bemused Hippie] * 1
    #29152264 -

Looks like mushroom mycelium struggling with bacteria. Some things you can find using the search feature if you haven't seen or tried them yet:
- Trenching
- Cabin sequestration
- Josex poke
- Water agar
- BRF pucks (personal favorite, though it requires a different set of ingredients)

On the germination plate, I favor the colony on the right. But why not try some from each? You can put multiple transfers on a plate.


--------------------
LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: psychedelicmind]
    #29152268 -

If your grains were a little over-hydrated, leaving the jar to consolidate can result in standing water. But watery jars are most likely bacterial. Do you have pictures of the jars? What did you inoculate them with?


--------------------
LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: psychedelicmind]
    #29152290 -

psychedelicmind said:
Is heavy condensation (pooling water at the bottom of the jar) a clear sign of bacterial contam?
My jars do hardly have any condensation until full colonization.
If i let the jars rest for 1 or 2 more weeks, the myc gets more and more dense and the condensation increases until there is pooling water at the bottom. Is it possible that this is only due to the temperature difference inside and outside the jar?


yeah, its possible due to the differential, but it will only serve to exacerbate the presence of any bacteria and make the grains soggy. a soggy jar has a much higher chance of being bacterial, or more bacterial. just don't get the correlation mixed up with the causation. I need a picture in order to give you a more detailed and helpful answer.


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29152292 -

Thanks for your feedback,
Phaethon said:
If your grains were a little over-hydrated, leaving the jar to consolidate can result in standing water. But watery jars are most likely bacterial. Do you have pictures of the jars? What did you inoculate them with?


Thanks for your feedback, Phaethon.
The jars were inoculated with spores, because i am still practicing doing agar...
Here are some pics of the jars:


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Re: Ask Quick Questions, Get Quick Answers [Re: psychedelicmind] * 1
    #29152295 -

those may be slightly bacterial, but it doesn't mean you wont get 2, 3 flushes. spiky growth and grains stuck to the bottom, but that could just be the water. send them.


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29152300 -

The Tao said:
Bit of wire, piece of dowel, screwdriver and pliers.



If you have a drill, it goes a lot faster.  I use stripped soft tie wire and $1 exacto handles.


--------------------
O son of Kunti, I am the taste of water, the light of the sun and the moon, the syllable om in the Vedic mantras; I am the sound in ether and ability in man.

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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head]
    #29152302 -

fiddle_head said:
those may be slightly bacterial, but it doesn't mean you wont get 2, 3 flushes. spiky growth and grains stuck to the bottom, but that could just be the water. send them.


Yeah, i have sent them already to shoeboxes.
The surface started to get some areas with yellowish teint and the growth of the myc does not look healthy. I am a little bit worried.

https://www.shroomery.org/forums/showflat.php/Number/29151930#29151930

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Re: Ask Quick Questions, Get Quick Answers [Re: psychedelicmind] * 2
    #29152324 -

yep, its not healthy unfortunately, but it doesn't mean it wont fruit. start getting other projects underway right now.


--------------------
----<><><>----

⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 1
    #29152331 -

fiddle_head said:
yep, its not healthy unfortunately, but it doesn't mean it wont fruit. start getting other projects underway right now.


Okay, thank you very much for your feedback!
Have a lot of other species and varities on agar.
I really hope to be successful in getting clean plates.

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Shop: Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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