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crispydatrapboy704
Friend

Registered: 12/21/24
Posts: 47
Last seen: 1 year, 4 months

Re: Ask Quick Questions, Get Quick Answers

[Re: crispydatrapboy704]

2
#29149253 - 03/06/25 10:54 PM (1 year, 8 months ago)

This is it.

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Splittinghairs

Registered: 02/17/25
Posts: 1,343

Re: Ask Quick Questions, Get Quick Answers

[Re: Splittinghairs]
#29149261 - 03/06/25 11:10 PM (1 year, 8 months ago)

Splittinghairs said:

LewDoja said:

If you make it as an all in one (AIO)... to keep from contaminating prior to full colonization

Quote:

Now the LC or agar might be fun to look into! That I need to figure out how to apply.

Dont worry about LC... agar though most definitely 👌.
Stipes "why agar".

Quote:

I took the chance of tin foil and cracking the lids. The lids were modified and I saw some folks say the micron filter can do vacuum things from sterilization. They all seem to be bouncing back but only time will tell.

Not sure what al this means, with the last sentence "bouncing back" right after "sterilization"????

I had already done the sterilization process for 16 jars. 4 seperate cultures used. Had a micron filter and injection point on lids

Out of all of them, two fully colonized while the others were iradict pace and no signs of contam and only at like 10% each. The mycelium that was there looked healthy.

I had read some folks say things about the filter sometimes vacuuming during the sterilization so I decided to wipe down tinfoil and place it over the lids and give them the quarter turn for GE

The APEs are now after 3 days about 30%, B+ is 50%. It’s only the TW that’s dragging so those just might be lost causes

Grabbing this post as a random update and just sharing info.

So twisting the lids open and adding the tin foil was the answer. Not sure what the hell with filters and all but I probably am never using them again.

Originally out of 16 jars only 2 did everything like they should have.

Once I opened the slackers up, 2 of the strains are off to bulk. The other two (slow colonizers) got up to 90%, no signs of contam and gave them one more BnS just to make sure they bounce back properly but I have no concerns atm over contamination as the mycelium looks healthy, no hard edges, and as it spread out it would link up with its buddy if it made contact so I think I’m good.

And all that after I had already decided it was time to just count those two as loses and prepped new stuff.

--------------------
Favorite Teks

]

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Jrey16
Magic Kabute

Registered: 12/19/23
Posts: 826
Loc: Philippines
Last seen: 1 year, 27 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Splittinghairs]
#29149329 - 03/07/25 01:24 AM (1 year, 8 months ago)

I think I already asked this question before but I have bad memory. After PCing jars how long can it sit before inoculating with agar? And it best to leave in the PC before. Inoculation or should I take the jars out? might not be able to inoculate for a few days. I use unmodified lids btw

--------------------

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crispydatrapboy704
Friend

Registered: 12/21/24
Posts: 47
Last seen: 1 year, 4 months

Re: Ask Quick Questions, Get Quick Answers

[Re: Jrey16]
#29149331 - 03/07/25 01:26 AM (1 year, 8 months ago)

Really it's good once it hits room temp tbh. 🤷

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fiddle_head
Shroomery Whinery Vintner

Registered: 08/05/08
Posts: 5,342
Loc: o
Last seen: 1 hour, 12 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Jrey16]

1
#29149345 - 03/07/25 02:00 AM (1 year, 8 months ago)

Jrey16 said:

I think I already asked this question before but I have bad memory. After PCing jars how long can it sit before inoculating with agar? And it best to leave in the PC before. Inoculation or should I take the jars out? might not be able to inoculate for a few days. I use unmodified lids btw

that is fine. i have rested jars at 6, 9, 12 mo. a few days is negligible. if you are going to be gone for a few days it may behoove you to remove them when cool, to avoid stagnant water.

--------------------
_{----<><><>----}_{⁂ tail-lights, back-pockets, rearview mirrors ⁂}

⁂ guides | grows | supreme air box | sporeaddict ⁂

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Jrey16
Magic Kabute

Registered: 12/19/23
Posts: 826
Loc: Philippines
Last seen: 1 year, 27 days

Re: Ask Quick Questions, Get Quick Answers

[Re: fiddle_head]
#29149353 - 03/07/25 02:21 AM (1 year, 8 months ago)

fiddle_head said:

Jrey16 said:

I think I already asked this question before but I have bad memory. After PCing jars how long can it sit before inoculating with agar? And it best to leave in the PC before. Inoculation or should I take the jars out? might not be able to inoculate for a few days. I use unmodified lids btw

that is fine. i have rested jars at 6, 9, 12 mo. a few days is negligible. if you are going to be gone for a few days it may behoove you to remove them when cool, to avoid stagnant water.

Thank you

--------------------

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,028
Loc: South Africa
Last seen: 3 hours, 25 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Jrey16]
#29149359 - 03/07/25 02:29 AM (1 year, 8 months ago)

I have a question that pertains to this, why would we be afraid of sterilized water in a pc?
Beyond rust and corrosion of the pc I'd assume the water would not be an issue or storing water for consumption would also be an issue.

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fiddle_head
Shroomery Whinery Vintner

Registered: 08/05/08
Posts: 5,342
Loc: o
Last seen: 1 hour, 12 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: DERRAYLD]
#29149362 - 03/07/25 02:32 AM (1 year, 8 months ago)

it's really not an issue, would it make any difference? certainly not. call it being paranoid or whatever D.

--------------------
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⁂ guides | grows | supreme air box | sporeaddict ⁂

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fiddle_head
Shroomery Whinery Vintner

Registered: 08/05/08
Posts: 5,342
Loc: o
Last seen: 1 hour, 12 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: fiddle_head]

1
#29149364 - 03/07/25 02:34 AM (1 year, 8 months ago)

congrats on your og tag too man i just saw that. well done.

--------------------
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⁂ guides | grows | supreme air box | sporeaddict ⁂

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,028
Loc: South Africa
Last seen: 3 hours, 25 minutes

Re: Ask Quick Questions, Get Quick Answers

1
#29149375 - 03/07/25 02:57 AM (1 year, 8 months ago)

Thanks Fid, it's something I've always wondered because I always have 60L of water on the counter for our daily use and people mention being concerned with standing water.

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maplepine
Stranger

Registered: 01/31/25
Posts: 70
Last seen: 10 months, 30 days

Re: Ask Quick Questions, Get Quick Answers

[Re: DERRAYLD]
#29149416 - 03/07/25 04:30 AM (1 year, 8 months ago)

Darn it, can someone help me remember where I saw a fun-looking tek? Someone was digging in a garden to inoculate for natural growth outside. It included manure compost I think.

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fiddle_head
Shroomery Whinery Vintner

Registered: 08/05/08
Posts: 5,342
Loc: o
Last seen: 1 hour, 12 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: maplepine]
#29149425 - 03/07/25 04:58 AM (1 year, 8 months ago)

that could be any thousands of posts but you could check the outdoor thread.

--------------------
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⁂ guides | grows | supreme air box | sporeaddict ⁂

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DV8DUG
Hyphal Exhibitionist

Registered: 12/27/23
Posts: 2,319
Loc: Melniboné
Last seen: 6 hours, 36 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Huskies]
#29149436 - 03/07/25 05:27 AM (1 year, 8 months ago)

Huskies said:

[...]
Went back to oats and I think I prefer it now, bigger but more robust growth, less kernels, more mass and water in each quart.

Hellz yes! GO OATS!

--------------------
Agar Plates and Removing Hidden Contaminants - A Visual NotebookRecognizing Contamination in Grain Jars - A Visual NotebookContaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation🅃🄴🄰🄼 🄲🄾🄽🄳🄸🄼🄴🄽🅃 🄲🅄🄿

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callmehippie
☮️🍄Hallucinogenic Hippie🍄☮️

Registered: 05/28/24
Posts: 3,517
Loc: Over the hills and far away

Last seen: 2 hours, 28 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Jrey16]
#29149461 - 03/07/25 06:54 AM (1 year, 8 months ago)

A couple weeks- up to about a month. As long as they don't contaminate it's really a matter of how fast they dry out.
Usually your good for a couple weeks. And starting to push it at 3 weeks to about a month.

Thats if they were hydrated properly when prepping.

--------------------
:hippie:

:hippie:

:mushroom2:

Take it easy. if it's easy. take it twice :mushroom2:

:mushroom2:

:mushroom2:

:sporedrop:

The Hitchhiker's Guide to the Shroomery

:sporedrop:

**A LINK TO EVERTHING**

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,028
Loc: South Africa
Last seen: 3 hours, 25 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: callmehippie]
#29149466 - 03/07/25 07:15 AM (1 year, 8 months ago)

You're still good at 8 months, hell 1 year or more of you present them from drying out.

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Headass

Registered: 07/24/24
Posts: 827
Loc: Texas

Last seen: 7 months, 18 days

Re: Ask Quick Questions, Get Quick Answers

[Re: DERRAYLD]
#29149544 - 03/07/25 09:34 AM (1 year, 8 months ago)

Quick ID ?

--------------------
Beef’s The Hitchikers Guide to Shroomery
Stipe’s Clean Spawn Checklist
Gummies by milkboy
Eat’s Easy Oats Prep
DV8DUG’s Recognizing Contamination in Grain Jars

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Grzybolud
Stranger

Registered: 08/24/24
Posts: 4
Last seen: 1 year, 8 months

Re: Ask Quick Questions, Get Quick Answers

[Re: Nichrome]
#29149573 - 03/07/25 10:12 AM (1 year, 8 months ago)

Hey

A friend of mine tried to grow Psilocibe natalensis. He didn't have any problems growing cubes and Natalensis also looked fine. For some reason 4 of the monotubs failed (mold etc.). All of the rye bags had a blue tint on mycelium. He initially thought it was psilocybin and bruising, but after 4 failed monotubs he thinks something was off from the beginning. I attach a picture of the grain after transfer which looks identical. Is it worth trying again or the blue tint is actually some type of mold? (I hope it's visible). Mycelium looks healthy overall.

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DERRAYLD
Constructus

Registered: 05/13/02
Posts: 15,028
Loc: South Africa
Last seen: 3 hours, 25 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Headass]
#29149576 - 03/07/25 10:13 AM (1 year, 8 months ago)

Looks like a Phobos or similar Mars isolate
Could be jack frost.

Headass said:

Quick ID ?

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tripdawg420
low life with no life

Registered: 02/03/09
Posts: 8,814
Loc: illinois
Last seen: 10 days, 11 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: Grzybolud]
#29149575 - 03/07/25 10:13 AM (1 year, 8 months ago)

thats contams

--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

:thumbup:

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Headass

Registered: 07/24/24
Posts: 827
Loc: Texas

Last seen: 7 months, 18 days

Re: Ask Quick Questions, Get Quick Answers

[Re: DERRAYLD]
#29149584 - 03/07/25 10:18 AM (1 year, 8 months ago)

DERRAYLD said:

Looks like a Phobos or similar Mars isolate
Could be jack frost.

Headass said:

Quick ID ?

Thank you!!

--------------------
Beef’s The Hitchikers Guide to Shroomery
Stipe’s Clean Spawn Checklist
Gummies by milkboy
Eat’s Easy Oats Prep
DV8DUG’s Recognizing Contamination in Grain Jars

Extras:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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