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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29140421 -

I would say it's good and that there was a different cause the first time.

Have you mapped out your laminar flow area?

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29140428 -

Axman said:
If you want to have a better chance at knowing your inoculating with clean media, agar is a good tool to have.

Also if you hang around the forums long enough (I believe 90 days, someone correct me if I'm wrong), and get your post count up to 50; you will gain access to the Marketplace. There's a lot of generous members who give away spore prints and cultures for free.



3 months vs 90 days small difference but someone recently was trying on 90days to no avail, so 3 months is more correct.


--------------------
A_wise_man said:
"Bad drugs tell you, that you want more;
  Good drugs tell you, that you've had enough"



🎶  KIND TC's - by: Tri  🎶

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29140433 -

Thanks, no I have not. I've done the lighter test around the unit, but not incense or anything. Do you mean testing the whole area for laminar flow using incense?

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Re: Ask Quick Questions, Get Quick Answers [Re: Hotboxer] * 1
    #29140435 -

I would be fairly confident in it, you can always run another control plate like you did with the rest of your transfers to see if anyone of it is flow related.

I've ran a few open air plates in my grow room, left the lid off overnight, then wrap it to see what grows. It usually takes a week or so for molds to grow, but bacteria is usually pretty quick.

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Re: Ask Quick Questions, Get Quick Answers [Re: Hotboxer]
    #29140444 -

Pretty much yeah, to see where you'll be best working at

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29140450 -

LewDoja said:
Looks good for the most part. Did it smell shroomy when spawned?

What tek did you follow for that tub?



Grain spawn did smell shroomy and good. The monotub has a standard design except that the filters are made of foam instead of polyfill or micropore. I made the monotub from two 6 qt Sterilite tubs which I customized to fit as a bottom and a lid. The principles are similar to any monotub.

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Re: Ask Quick Questions, Get Quick Answers [Re: tom of alchemy]
    #29140469 -

As long as my coir is properly prepped to field capacity I can spawn to shoeboxes (got the 16qt versions) pit the lid on and truly set and forget them until pinning?

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Re: Ask Quick Questions, Get Quick Answers [Re: YouEnjoyMyself41]
    #29140472 -

Pretty much yeah,  but environment dependent, as well as coir quality.

Still has ge from between lid?

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaedra]
    #29140491 -

Thanks for answering! This information was very helpful!

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Re: Ask Quick Questions, Get Quick Answers [Re: YouEnjoyMyself41] * 1
    #29140577 -

Not until you have things dialed in. Every grow space is unique.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29140593 -

So, I have this jar of millet that I inoculated with an old agar plate that I've been keeping in the fridge for some time, but it never started. It's from 11/01, do you think that I can use it for another inoculation from a "good" plate? It doesn't show signs of contamination, as far as I can tell.



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Re: Ask Quick Questions, Get Quick Answers [Re: Nerex]
    #29140617 -

Are these oats looking good?



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Re: Ask Quick Questions, Get Quick Answers [Re: Mub]
    #29140660 -

Mub said:
Hmmm ok. Is it worth even using the grafting tape than?



I wrap around the plate about 1.5 times with grafting tape, then just over 1 times with micropore tape. Usually fold over the last bit of micropore, to make it easy to remove.

I started doing this after I had some grafting tape unravel while the plate was sitting and growing.

Probably was semi-dried grafting tape, some only use grafting tape (it sticks to itself) but if you use both, it will stay shut. 

Here are plates wrapped with both.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61]
    #29140708 -

Should I pitch my agar if it is too stiff?  Tried to inoculate my plates today and the agar was very stiff and brown. I did a few of them but not all. It just crumbled when I used my loop.

Should I pitch the rest of them and start over?

I was using this mix. It said 5 scoops to 500ml of water and had me boil the mix, and remove from heat as soon as it started to boil.
https://www.amazon.com/gp/aw/d/B0DDWXPH5N?psc=1&ref=ppx_pop_mob_b_asin_title

I at least saved some of my syringe if I have to start over.

Edited by Mub (02/27/25 08:16 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub] * 1
    #29140720 -

I think you used twice the recommended amount (5 scoops or 50 g per liter, not per 500 ml).

You can always see if anything grows. But next time use a scale (scoops are not going to be very consistent) and use the prescribed amount.

Edit to point out that the components are cheap and worth buying separately imo. You can find agar agar powder and light dry malt extract easily on Amazon, and mix them at 2% each, e.g. 500 ml water, 10 g agar agar powder, 10 g malt extract. The premixes are generally ripoffs.

Edited by Phaethon (02/27/25 08:33 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29140725 -

:whathesaid:
I second the using of scale for consistency.
Also the ability to mix different ratios for different needs...


You can add water to it and re-sterileize but after already pouring some plates it will be guessing game...

Edited by LewDoja (02/27/25 08:36 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29140740 -

Phaethon said:
I think you used twice the recommended amount (5 scoops or 50 g per liter, not per 500 ml).

You can always see if anything grows. But next time use a scale (scoops are not going to be very consistent) and use the prescribed amount.

Edit to point out that the components are cheap and worth buying separately imo. You can find agar agar powder and light dry malt extract easily on Amazon, and mix them at 2% each, e.g. 500 ml water, 10 g agar agar powder, 10 g malt extract. The premixes are generally ripoffs.



This is exactly what happened I used twice the amount. At least I caught it and didn’t use all my syringe.  I had two 500ml I was going to use but swapped them out and forgot, those are what I used to measure my water. I’m 100% not getting the pre mix. It just seemed like it would be easier. I already have a scale too.

I did innoculatr three dishes. Are those just fucked or are they worth keeping?

Edited by Mub (02/27/25 09:47 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mub]
    #29140763 -



Would you send this jar? Gonna give it a couple more days to consolidate likely.


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Re: Ask Quick Questions, Get Quick Answers [Re: bluespirit13]
    #29140764 -

bluespirit13 said:


Would you send this jar? Gonna give it a couple more days to consolidate likely.



I'd give it another day or two to see if it colonizes those dry cracked grains, and then send it.

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Re: Ask Quick Questions, Get Quick Answers [Re: bluespirit13]
    #29140768 -


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