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Re: Ask Quick Questions, Get Quick Answers [Re: psychedelicmind]
    #29139601 -

I'm not sure if the peptone is having an effect here or what.

Just doesn't look right.... (could be contamination enmeshed)

Wait to see what others have to say.

But yeah definitely will want to start some new plates.

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Re: Ask Quick Questions, Get Quick Answers [Re: mike-ologist]
    #29139603 -

Pics?

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29139613 -

So if you pre soak your grains and your substrate is dry could you strain some of the water from your grains into the substrate for field capacity?

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs] * 1
    #29139622 -

Splittinghairs said:
So if you pre soak your grains and your substrate is dry could you strain some of the water from your grains into the substrate for field capacity?



I personally wouldn't.

The grain soak water may have nutrients in it, people use it for LC, agar, all sorts of stuff, but it is usually sterilized for that.

There is often a bit of bioactive things inside the grain soak water that I would assume would raise your chance for contamination tremendously.

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman]
    #29139646 -

Axman said:
suicideprevention said:
How does this shoebox look? Grain to coir 1:1.5. Lid has been snapped closed since spawn was mixed to grain except for when I took this pic. I have other box with better pin growth and another with less. Now that I have pins can I safely open the box to mist? Should I mist? Or so I just latch it closed and keep it as it was?



Misting is only necessary if you don't first set your field capacity correctly, perfect hydration on spawn would have little microscopic water droplets on top of your whole sub, if it is drying up there will be a noticeable color change in your substrate. I personally would LITFA (Leave It The Fuck Alone). It's looking good, and lot of things can change your pinset, genetics is one of them.



Ok thanks for the tips. The pins have exploded and fruits are just about to the lid. At this point do I just flip the lid upside down and let the fruits push on the lid?  Or what exactly should I do?  Unfortunately I don't have any more shoeboxes so I can't make a dubtub set up and I'll need to do something tonight because they will be pushing against the lid by morning

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Re: Ask Quick Questions, Get Quick Answers [Re: suicideprevention] * 2
    #29139650 -

I do have a 66qt mono that I made using the EZ dialed tek, there is nothing in in right now, I could probably stick all 3 of my shoeboxes in there with the shoebox lids off but the mono lid on?

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Re: Ask Quick Questions, Get Quick Answers [Re: suicideprevention] * 1
    #29139655 -

When I'm in that situation and I catch it before they touch I just pop the lid and let it rest on top, the mushrooms will push it out of their way as they grow.

Or if I'm gone and don't get to it in time, the mushrooms will just kinda grow along the top of the lid. It's not super detrimental either way.

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29139656 -

Axman said:
Splittinghairs said:
So if you pre soak your grains and your substrate is dry could you strain some of the water from your grains into the substrate for field capacity?



I personally wouldn't.

The grain soak water may have nutrients in it, people use it for LC, agar, all sorts of stuff, but it is usually sterilized for that.

There is often a bit of bioactive things inside the grain soak water that I would assume would raise your chance for contamination tremendously.



Can confirm.

Think of the etymology of germ-inate. The grains all have germ pores that release microbials beneficial to plant growth. We soak grains,in part, to release these in an attempt to lower the final count of microbial that are pcd to death, thus reducing the chance something makes it through the pc cycle.

Typically, if your sub is a bit dry, I'd mist with distilled water.


--------------------
Check out my wish list and let me know what you got! UPDATED: 2/22/25
Current wish list


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Re: Ask Quick Questions, Get Quick Answers [Re: suicideprevention] * 2
    #29139657 -

suicideprevention said:
I do have a 66qt mono that I made using the EZ dialed tek, there is nothing in in right now, I could probably stick all 3 of my shoeboxes in there with the shoebox lids off but the mono lid on?



:bigyesnod:
Yes

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Re: Ask Quick Questions, Get Quick Answers [Re: mike-ologist]
    #29139667 -

mike-ologist said:
Axman said:
Splittinghairs said:
So if you pre soak your grains and your substrate is dry could you strain some of the water from your grains into the substrate for field capacity?



I personally wouldn't.

The grain soak water may have nutrients in it, people use it for LC, agar, all sorts of stuff, but it is usually sterilized for that.

There is often a bit of bioactive things inside the grain soak water that I would assume would raise your chance for contamination tremendously.



Can confirm.

Think of the etymology of germ-inate. The grains all have germ pores that release microbials beneficial to plant growth. We soak grains,in part, to release these in an attempt to lower the final count of microbial that are pcd to death, thus reducing the chance something makes it through the pc cycle.

Typically, if your sub is a bit dry, I'd mist with distilled water.



Ok. Thanks to you both!

My brain was stuck on the logic of grains colonized means no contam (in theory) so figured the water soak should be from “clean” water. But both of your posts confirmed that nagging voice in my head.

Now the LC or agar might be fun to look into! That I need to figure out how to apply. I have only ever had luck with GT and right now I have 3 others working.

But then again I’m not sure how well the strains from these guys would be. They were from a LC trusted source, but they kinda stalled out and after much digging through here I took the chance of tin foil and cracking the lids. The lids were modified and I saw some folks say the micron filter can do vacuum things from sterilization. They all seem to be bouncing back but only time will tell.

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29139681 -

If you want to have a better chance at knowing your inoculating with clean media, agar is a good tool to have.

Also if you hang around the forums long enough (I believe 90 days, someone correct me if I'm wrong), and get your post count up to 50; you will gain access to the Marketplace. There's a lot of generous members who give away spore prints and cultures for free.

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs] * 1
    #29139781 -

If you make it as an all in one (AIO)... to keep from contaminating prior to full colonization

Quote:
Now the LC or agar might be fun to look into! That I need to figure out how to apply.



Dont worry about LC... agar though most definitely 👌.
Stipes "why agar".

Quote:
I took the chance of tin foil and cracking the lids. The lids were modified and I saw some folks say the micron filter can do vacuum things from sterilization. They all seem to be bouncing back but only time will tell.




Not sure what al this means, with the last sentence "bouncing back" right after "sterilization"????


Edited by LewDoja (02/26/25 11:52 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: suicideprevention]
    #29139788 -

suicideprevention said:
Axman said:
suicideprevention said:
How does this shoebox look? Grain to coir 1:1.5. Lid has been snapped closed since spawn was mixed to grain except for when I took this pic. I have other box with better pin growth and another with less. Now that I have pins can I safely open the box to mist? Should I mist? Or so I just latch it closed and keep it as it was?



Misting is only necessary if you don't first set your field capacity correctly, perfect hydration on spawn would have little microscopic water droplets on top of your whole sub, if it is drying up there will be a noticeable color change in your substrate. I personally would LITFA (Leave It The Fuck Alone). It's looking good, and lot of things can change your pinset, genetics is one of them.



Ok thanks for the tips. The pins have exploded and fruits are just about to the lid. At this point do I just flip the lid upside down and let the fruits push on the lid?  Or what exactly should I do?  Unfortunately I don't have any more shoeboxes so I can't make a dubtub set up and I'll need to do something tonight because they will be pushing against the lid by morning




Can use a small refuse can liner, grocery bag, or a larger tote that this one will fit inside and make a water tub with it.

But yes you can just leave the lid snapped (if it would dry out too much) or un-snapped (if not) I dont mind the fruits hitting the lid they'll figure something out.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29139810 -

I have a mutant strain I've been developing, I've called it The Thing. This is the original mutant pseudocarp. I cloned it.



First tub came out like this, even the caps were quite a different pheno compared to my normal Husky. I do have prints of those, I haven't tried them yet, but I guess I should.







After that tub, most tubs ended up looking more like this, unfortunately. Pain in the ass to harvest.





Then this tub gave me some very interesting clone samples, I called it Beastlord and Flowerpower. Beastlord was 100g, Flowerpower around 60g





.

I also took a single swab of Beastlord and put it on agar. SHould've done more ooops. Is it possible that instead of spores, I actually just cloned some gill tissue without realizing?

So these MS plates looked very similar to my original The Thing plates, which surprised me. I was expecting a revert to more normal looking pins, but it is giving me the pseudocarp blobs.



So currently, I have a few Flowerpower tubs and Beastlord shoeboxes. Unfortunately, These are both expressing the standard The Thing pseudocarps and not the cool mutants I was hoping for, at least so far.

Beastlord



Maybe some of these pins will turn into a gigantic mutant?



And my first tubs of the MS The Thing, which was from the Beastlord clone, are ALSO looking like the original The Thing. Still early, but this is surprising to me.



I know Enigma doesn't have spores, but The Thing does. Are heavily mutated strains likely to pass on those genes via spores? Think F2s would be different? Has there been more heavily mutated strains like these that were stabilized via spores, or am I surprisingly the first?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29139820 -

Yes it's possible you've cloned via gill fragments. How long till signs of growth?

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29139824 -

It was pretty quick tbh, but I don't remember. Would you guess its based on how long it was?

How many days is it for spore to show growth  if they are fresh vs something like accidentally cloning gills? I assumed the quick growth was because they were fresh and stronk.

Now I'm wondering if most of my swabs have ended up just being that hehe. Shit.

I'm lucky I have spore prints though. I guess I'll have to grow those out to compare. If the spore print did legitimately yield the same results, would that be an oddity?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

Edited by Huskies (02/27/25 12:11 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29139828 -

If the temperature is stable and it’s kept in a dark place does DMT still need to be kept in the freezer to prevent it losing its potency?

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29139830 -

No, how long are you planning?

It may turn darker but it's still good.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29139841 -

I am not completely ready. It’s there in a dark safe place that I know about.
But it’s been there for a couple of months and I’m just wondering if I’m losing anything. I don’t mind if it gets dark.
I’m building up to it. I’m getting close to being ready to try it.

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29139847 -

Have you tried low doses?

I love sub-ohmed low doses

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