BonsaidMycelium said:

Thanks man. What is most shocking to me is how quickly the Starry Night colonized the BR all the way on the left. I did them all on 2/15. It will be fully colonized by tomorrow night it looks like.

Any special considerations for the strain? Im having good success with cubes in the fahtzip method, should be seeing pins in a day or two. Do SN/APE types need the same kind of process or something different?

Thanks!

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Mark_M said:

Two quick questions.
1) The fruit on the top of this mound stopped growing a week ago before any of the other fruit on the sides started pinning. Should I harvest, toss or leave these presumably aborted fruit on top?

2) These sporilated onto their skirts so I removed the skirts and figure I could use them to start another batch from directly; is this a possibility and what would be the simplest or best way to go about it? Thanks!

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Axman said:

Mark_M said:

Two quick questions.
1) The fruit on the top of this mound stopped growing a week ago before any of the other fruit on the sides started pinning. Should I harvest, toss or leave these presumably aborted fruit on top?

2) These sporilated onto their skirts so I removed the skirts and figure I could use them to start another batch from directly; is this a possibility and what would be the simplest or best way to go about it? Thanks!

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1. It's hard to picture which one you are talking about but if the cap changed color and it stopped growing than it is most likely an abort. You can either toss or leave the mycelium will take care of it how it sees fit.

2. Unless you're planning on putting the spore coated veil onto agar, I don't see a use for them. Since fruiting is done in open air they are in contact with a fair bit of contamination. Any efforts to put them to grain would most likely end in contamination. The best course of action would to take a spore print from one, and also a biopsy from the inside of the stipe to agar.

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1) See red circle below... My inclination has been to leave to the mycelium as you suggest, unless they might have meaningful content...


2) Thanks. Have not done anything yet with agar but might be a fun experiment. I imagine you'd know pretty quickly if you had contamination, yes? Thanks again - Mark

Mark_M said:

1) See red circle below... My inclination has been to leave to the mycelium as you suggest, unless they might have meaningful content...


2) Thanks. Have not done anything yet with agar but might be a fun experiment. I imagine you'd know pretty quickly if you had contamination, yes? Thanks again - Mark

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Axman said:

Mark_M said:

1) See red circle below... My inclination has been to leave to the mycelium as you suggest, unless they might have meaningful content...


2) Thanks. Have not done anything yet with agar but might be a fun experiment. I imagine you'd know pretty quickly if you had contamination, yes? Thanks again - Mark

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1) Content? as in psilocybin? Quite possibly, but I'd recommend grabbing them before they start to rot if you so desire. They do look like they are aborting.

2) Agar is the best way to test for contamination as well as germinating spores, in my opinion.

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Sarnath said:

Phaethon said:

If you leave the lid on, you can take tissue samples from the substrate itself, and clone whole mushrooms (even the outside should be sterile if it's never contacted unfiltered air). They won't grow very pretty without fresh air, though.

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"From the substrate itself", as in taking a small sample of the mycelium surface with an inoculation loop and putting that onto some agar?

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psilygoose said:

How much does dry rye increase in volume during prep? I.e., I know wheat roughly doubles, so I measure 3 jars to dry to make 6 prepped jars, is rye the same? Thanks!

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psychedelicmind said:

Can you have a look at my agar plates, please?
It's cubensis 'Puerto Rico' 2nd transfer was made on february, 8th.
Agar recipe: 500ml water, 10g agar, 7,5g malt extract, 0.5g peptone
Not sure if it is pure cubensis myc or if there is anything else growing.
Before i started to use peptone in my agar the myc was also tormentose, but it looked more organized.
Thanks in advance!

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LewDoja said:

@maple

"Pics or it didn't happen"

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Haha OK!

maplepine said:

LewDoja said:

@maple

"Pics or it didn't happen"

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Haha OK!

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Axman said:

While you wait for your first flush to come in I recommend looking into Water Tubs combined with Fahtlocs for optimal PF tek fruiting conditions. As shotgun perlite tubs are old news and we are trying to phase them out.

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But I have a whole bag of perlite! LOL

Thanks for the tip. I've had a hard time finding the most recent/recommended methods among all the content here