Banfir said:

Could a TC or others give me a quick gut check on this?  Trying PF Tek for the first time using a water bath and Verm for the roll after the dunk. These were birthed about 10 days ago.  Everything was going smoothly, but I’m a little nervous these look bacterial in the past few days. Any advice appreciated.

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ldmulti said:

Expanding volume of LC. PE LC (mycelium) after 6 days incubation. Media is crystal clear. I may have been too aggressive when shearing the mycelium? The blue spots only appear on the mycelium that is stuck to the jar, at/above the “media line” (? Top of the liquid). Looked all over the forums, could not find a similar post. Thank you for your assistance🙏🏻

Leeloo

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Jrey16 said:

I got a Golden Teacher plate sent to me from overseas and made 3 transfers and this is how it looks.
Do these plates look okay? I am used to rhizomorphic growth. I know there is different type of mycelium, is this considered tomentose mycelium? I just made a few more transfers now and I'm thinking of transferring again to agar with lower nutes to maybe get more of that rhizomorphic growth. Or is there no need to?

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Peleus said:

Found this tea tek by PrimalSoup: https://www.shroomery.org/forums/showflat.php/Number/12494141#12494141 . Is it still recommended? If I read it right it goes with one third of fresh shroom weight in water 2-3% in citric acid (lemon juice?). Bring to boil, mash and boil for 1 min,  let stew for 10 mins, strain and enjoy.

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Peleus said:

Found this tea tek by PrimalSoup: https://www.shroomery.org/forums/showflat.php/Number/12494141#12494141 . Is it still recommended? If I read it right it goes with one third of fresh shroom weight in water 2-3% in citric acid (lemon juice?). Bring to boil, mash and boil for 1 min,  let stew for 10 mins, strain and enjoy.

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QuantumZ00 said:

This is the one I used until recently when Northerner directed me to a simpler functional way that was just as potent, but easier for me to dance around the horrid taste. But yes, this worked for me up until i switched the recipe.

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Kloakk said:

Rhizo or tomentose doesn't really tell anything about if it will do good or not.

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LewDoja said:

However it's much easier to spot ride alongs when you have a consistent flat rhizo culture. Plus it looks cool.

Never have I've been disappointed with a gnarly looking rhizo culture.

Just my 2cents

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Couldn't agree more

ldmulti said:

Absolutely, agreed… and thank you for taking the time to reply.

I should have prefaced the post with more info… shouldn’t be posting while tired 🫠

This is one of 6 jars I prepped using a syringe (commercial) that I tested on agar and was beautiful and clean. Only this one jar has the mold and I was mostly curious if bruised mycelium had been reported previously re: liquid culture. I managed a cell/molecular lab for nearly 20 years and my specialty was antibiotic-free neural stem cell culture. I am most comfortable using liquid cultures, although proficient with agar as well.

I did not take your statement to be dismissive, in fact, I appreciate critical feedback very much. I became used to defending my articles/methods/whatever and understand that you have to walk on eggshells just to help out others even though they asked for the help in the first place.

Thank you again for the reply I will test on agar for good practice, and if I have extraordinary results, I will be sure to post.

Leeloo

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That's a mesmerizing picture - neural stem cells with some kind of contrast dye?

If that LC turns out not to be moldy, I'd love to see the test plate. I've never seen mycelium in LC bottles turn blue from bruising, but I have seen bruised mycelium on grain spawn so it may be possible.

Are you using a flow hood/FFU or still air box? How did you inoculate the liquid culture jars? I'm assuming you know how to sterilize media (and anyway mold doesn't even survive boiling temperatures), so if the donor LC tested clean, it was probably introduced at inoculation. Maybe there's an easy modification to your sterile technique here.

I like using unmodified media bottles (~half filled leaves enough air that it's usually not even required to leave the lid loose for gas exchange during colonization). If you prefer to use jars, one-piece plastic lids are a little easier to handle without touching surfaces that need to stay sterile.