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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40] * 2
    #29120491 -

catnip40 said:
ParanoidGuy said:
I want to attempt to cross P. ochraceocentrata with P. cubensis. Do I have a chance if I try to mate two isolated dikaryons together on agar?


from what I've read they are not that close 6-7 base pairs in difference which seems to cause outbreeding depression

u cant really truly breed two dikaryons together like that either, but there are some old research papers that seem to suggest u can do like in vitro dedikaryotization>pairings that way via truama/agitation but its hard to say whats actually going on if u take that route, and u cant really confirm it that way either


just want to add a little context to this to avoid confusion

while you can't truly cross two dikaryons, you can put two dikaryons together and cause one or potentially both to dedikaryotize and pair. there is esoteric literature that explains this process, the only thing is no such thing or very much genetic/breeding stuff has been published in regards to cubensis or psilocybe in general

Muad.Dweeb and trippieblack both talk about this. as well as fahtster

most people will say this type of crossing is akin to ghetto swabbing and unverified, not consistent, or whatever.(which i don't think there is anything wrong with double swabbing just saying some ppl "look down" on it) and adamant monokaryon crossers will tell you it is not possible

trippieblack explains how you can verify this under a scope but I have not really looked into this aspect much

while isolating and pairing monokaryons might be a more systematic and verifiable approach. pairing dikaryons thru in vitro DDK can lead to potentially multiple recombinants at once.. which means you get to pheno hunt and select more things right off the bat because u are working with 4 nuclei at once instead of 2 in a mon x mon pairing


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∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

Edited by catnip40 (02/10/25 07:44 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 2
    #29120504 -

Ah three months... Okok. I'll cough up some more patience then


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle] * 1
    #29120597 -

Hello!
According to Murphy's law, anything that can go wrong will go wrong, or in mushroom cultivation terms: your mushrooms need to be harvested at the worst possible time. Well, it happened (maybe, that's why I'm here) to me. My girlfriend's (she is okay with me cultivating) friend is coming over this evening, and staying for the night and to tomorrow evening, so about 24 hours. The friend is probably fine with knowing that I cultivate, but I would rather not want anyone else but my girlfriend to know. We live quite small, so I wont be able to harvest when the friend is here (maybe I can find a window to harvest tomorrow at noon).

My question is the following: Do I pick the most ripe mushrooms now and place them on the dehydrator and put the tub in the wardrobe, or do I place the tub in the wardrobe and wait 24 hours?


Thanks and moosh love:heart::mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: capsap] * 1
    #29120600 -

Does anyone know what is the source of the fin mutation? Seems like its the origin of enigma, and my own The Thing. Just noticed a new fin mutation in a PExGhost tub.

This the the thing.



--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: capsap] * 2
    #29120602 -

capsap said:
Hello!
According to Murphy's law, anything that can go wrong will go wrong, or in mushroom cultivation terms: your mushrooms need to be harvested at the worst possible time. Well, it happened (maybe, that's why I'm here) to me. My girlfriend's (she is okay with me cultivating) friend is coming over this evening, and staying for the night and to tomorrow evening, so about 24 hours. The friend is probably fine with knowing that I cultivate, but I would rather not want anyone else but my girlfriend to know. We live quite small, so I wont be able to harvest when the friend is here (maybe I can find a window to harvest tomorrow at noon).

My question is the following: Do I pick the most ripe mushrooms now and place them on the dehydrator and put the tub in the wardrobe, or do I place the tub in the wardrobe and wait 24 hours?


Thanks and moosh love:heart::mushroom2:


those could go another 24 hours, i would probably let em grow

you could harvest em now too if you wanted though


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∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

Edited by catnip40 (02/10/25 10:37 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29120603 -

Huskies said:
Does anyone know what is the source of the fin mutation? Seems like its the origin of enigma, and my own The Thing. Just noticed a new fin mutation in a PExGhost tub.

This the the thing.




enigma is a mutation that comes from a pe x B+ cross called tidalwave#2

here is some good info on blobs

Update on Pseudocarps (blobs)


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∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40] * 1
    #29120613 -

I over filled some jars last week and they swelled up more than I anticipated during the PC.

Today I’m transferring the WBS to bags and I’m going to PC again. Do I really need to pc for 90 minuets again or would a shorter PC be ok?


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Ask Quick Questions, Get Quick Answers [Re: OLDDAD77]
    #29120618 -

is it so bad the jars are unable to be shaken?

if you are going to re pc ya you need to do another full cycle at least 90

i have never repc grains so im not sure how that goes


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∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: OLDDAD77] * 3
    #29120621 -

Mwj12977 said:
I over filled some jars last week and they swelled up more than I anticipated during the PC.

Today I’m transferring the WBS to bags and I’m going to PC again. Do I really need to pc for 90 minuets again or would a shorter PC be ok?


The whole schebang is sketch, personally I'd just restart with new grain.  Any time you want to sterilize something it's full cycle, there are no half measures.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] * 3
    #29120644 -

Yeah that’s what I figured. Fuck it I’ve got enough room to shake it’s just gonna be a bitch.


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Ask Quick Questions, Get Quick Answers [Re: OLDDAD77] * 3
    #29120646 -

Mwj12977 said:
I over filled some jars last week and they swelled up more than I anticipated during the PC.

Today I’m transferring the WBS to bags and I’m going to PC again. Do I really need to pc for 90 minuets again or would a shorter PC be ok?


Two options I see are:

1. Dump some grain out during inoculation and use the jar as normal.

2. just start fresh for the bags.

I would fully expect a second PC cycle to ruin your grains, probably ending up with a large amount of burst grains.

There is no safe way to cut corners in mycology, let alone during the sterilization steps.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 1
    #29120653 -

Yeah I wasn’t trying to cut corners just didn’t want a bunch of burst grains. This is what I get for trying to switch from rye. Thanks for the feedback back you guys answered my question.


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 2
    #29120654 -

Axman said:
I would fully expect a second PC cycle to ruin your grains, probably ending up with a large amount of burst grains.


I respectfully disagree on that redoing a PC cycle on your grains will ruin your grow. I'm heavily in the camp that while not ideal, a lot of burst grains don't harm your mycelium at all. Hell, I've had amazing grows on sprouted and mushy black quinnoa that I've run through a 2 hour cycle at 18 psi after a far too long soak :smirk:  (I really liked the color contrast of white mycelium on black quinnoa so I got a 5kg bag couple years ago)

In my experiece; sterile is sterile, doesn't really matter if the grains are burst - the mycelium actually likes some of that exposed starch and you're pretty sure that they're not underhydrated;



I don't have any proof but this jar did excellent!

Quote:
There is no safe way to cut corners in mycology, let alone during the sterilization steps.


This is very true!

Edit: so I also agree that he should do a full PC cycle worth 90 minutes again. PCing for 45min twice does not make one full 90min cycle :stoned:

Edited by Throwaway (02/10/25 11:17 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: OLDDAD77] * 1
    #29120656 -

WBS definitely expands :thumbup:


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29120658 -

wbs expands about 1/2 more than u started with give or take

burst grains aren't the end of the world unless its so bad u end up with a brick


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 1
    #29120661 -

I vent for 10 minutes PC for 2hrs.

Not a fan of burst grains because of how goopy they make my jars. They definitely still work, but if the intention is to make the grain more shake able I feel like it's counterproductive.

I'm also team Rye.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Axman] * 4
    #29120668 -

There's 2 type of people in this world grain perfectionists and people who grow

Just jokes !! :lolsy:


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40] * 4
    #29120671 -

catnip40 said:
There's 2 type of people in this world grain perfectionists and people who grow

Just jokes !! :lolsy:


There's a difference between what I want to do, and know I should do... and what my grains look like :rofl:.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: Throwaway] * 2
    #29120677 -

Quote:
Throwaway said:



I kept looking for myc jumping off that "agar" ...


QQ: Does anyone know how many calories are in cubensis? I'm starting to lose my figure on the astral plane :/


--------------------
MUSHROOMS RULE

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Re: Ask Quick Questions, Get Quick Answers [Re: Dala] * 1
    #29120692 -

I would guess that in the ballpark of other mushrooms. Like maybe roughly in the range of 200 - 300 kcal per 100g dried. I guess you can’t put the blame on them.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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