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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 2
    #29111791 -

I moisturize after washing so my dry skin isn't shedding all over, especially in the dry winter.

Accidentally brushing the edge of arm holes illicits mental images of skin and yeast flying around my SAB

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Re: Ask Quick Questions, Get Quick Answers [Re: Myndful]
    #29111807 -

Myndful said:
What's the best protocol for working with plate pins?

Do I wait for the plate pins to grow out and transfer?



If I'm trying to clone I take it before the cap opens. I chop of the cap to keep it from opening and dropping spores, and chop off the bottom to keep from having mycelium that's not the same culture as the clone.

I push that headless footless plin stem into a fresh plate.

If any plins have dropped spores in the the doner plate, it's going to be more work to get a clone from that stem. May be contammed by spores. (not clone)


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: aShogunNamedMarcus]
    #29111811 -

Awesome input Puff & Shogun.

Emphasizing the "What works best for YOU"....

That we all need to Dial-in ourselves and can't be done for us, but we can share our results and reasons, in hope others may find it's relevancy.

Such as the scraping against arm holes, many may not have thought of that.... is great Pro-tip.

I melted the edges of mine after using hole saw so they are nice and smooth. Seeing some of these jagged SABs make my skin crawl- ouch.

@Puff  how do you store your sleeves and how often to change out?
Do you wipe down with iso first or during?

I'm a fan of the Tyvek paint suits for filters, or even just cover for a sfd and ship so you don't risk poking into a dirty port.

Have used the sleeves from those many of times

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29111840 -

LewDoja said:
@Puff  how do you store your sleeves and how often to change out?
Do you wipe down with iso first or during?



I keep the sleeves in a ziploc. I'm sure something from the air gets on them but it can't be that bad. I used a pair of sleeves per sab session, so if I'm doing many jars I'll take them off/put them on multiple times. I throw them away when done. I use fresh gloves every time I take them off.

I used to ISO my gloves and HG agar plates, but I ran out and did some work without it and didn't really notice a difference.

Over the last year I've been dealing with contamination issues in my poured agar, grain, and tubs.

I've tried Iso, Bleach, soapy water, lysol spray. I forget who, but one of the TCs mentioned they run a dry SAB. I decided to give that a try and just be really mindful of my movements inside the SAB. It made a huge difference overall. I'd still sometimes get some biofilm on my plates, even after I'd wash my hands to the elbow and douse myself with ISO. I decided to try gloves and sleeves and now I rarely get any contamination on my plates now.

I'm still getting some in my tubs, but I think it might be related to the Oats I was using, that or hidden bacteria intertwined with mycelium. I still have cultures going from when my failure rate was higher, so it is possible I've been carrying those microscopic fuckers around with me this entire time.

I don't use Iso at all anymore and I'm not seeing bacteria show on my plates at all unless I accidently put my gloved finger inside the plate.

I recently had some green and grey mold show up on a few plates, but I realized I worked with some moldy spore swab plates the week prior without washing my SAB out. I'm 99% sure that the mold got into the SAB from those plates and I didn't let it settle long enough before working with the other plates.

Overall, 'less is more' seems to work for me when it comes to my sterile work.

My line of thinking is that anything we use to disinfect only kills 99.9% of bacteria and zero mold spores. That still leaves hundreds of thousands, if not millions of bacteria on every square inch of our body. Being mindful of what you do in a SAB, and a barrier between your work and your body is far more effective than any sanitizer.

It seems to be working for me. I still havent figured out how to take proper pictures of my agar plates, so I can't offer any proof, but if the shear amount of failure I've seen has taught me anything it's how to know when something is off.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Edited by PuffM4gicDr4gon (02/02/25 09:22 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 1
    #29111861 -

Does sporulation on the substrate lower the yield for next flush?

My maza isolate gave me 400+ grams wet on first flush, I expected to have a bigger second flush but all I have is barely 20g wet. It was full of spores from the first flush.

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Re: Ask Quick Questions, Get Quick Answers [Re: ParanoidGuy] * 2
    #29111865 -

ParanoidGuy said:
Does sporulation on the substrate lower the yield for next flush?

My maza isolate gave me 400+ grams wet on first flush, I expected to have a bigger second flush but all I have is barely 20g wet. It was full of spores from the first flush.


No. There are very old posts that claim this to be true, but I have not found it to be true, and same with others.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: ParanoidGuy]
    #29111869 -

ParanoidGuy said:
Does sporulation on the substrate lower the yield for next flush?

My maza isolate gave me 400+ grams wet on first flush, I expected to have a bigger second flush but all I have is barely 20g wet. It was full of spores from the first flush.


Absolutely nothing positive happens from spores dropping on the first flush... I would WAY rather harvest before the spores drop than risk a little more growth with a ton more spores dropping.  On the second flush I grow to the max because I don't want the third flush.


--------------------
“Order is needed by the ignorant but it takes a genius to master chaos.”
― Einstein


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Re: Ask Quick Questions, Get Quick Answers [Re: ParanoidGuy]
    #29111895 -

:whatshesaid: (Nektar)

400 wet seems like you got a lot with in the first flush which, according to some, is the Way it's supposed to be (if everything is done right) .

Did you punish your sub after harvest or at least give it a good dunk?

Maz is a heavy sporer but that not the reason.

What grain spawn?

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29111909 -

Did my second flush produce less fruits because I let the first flush sporulate all over the place?
400g first flush, second flush: 20g wet.

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Re: Ask Quick Questions, Get Quick Answers [Re: ParanoidGuy] * 5
    #29111913 -

No your second flush produced less because your first flush was dope as fuck. With a perfect culture, one flush is ideal.

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Re: Ask Quick Questions, Get Quick Answers [Re: ParanoidGuy] * 3
    #29111919 -

ParanoidGuy said:
Did my second flush produce less fruits because I let the first flush sporulate all over the place?
400g first flush, second flush: 20g wet.


As Supa said, there just wasn’t much left.

Spores do not effect later flushes.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: ParanoidGuy] * 1
    #29111960 -

Smellyhobbit said:
As Supa said, there just wasn’t much left.


💯 

@Paranoid- ^ hence the questions
Quote:
Did you...... at least give it a good dunk?
What grain spawn?



I understood what you were asking....
Quote:
Maz is a heavy sporer but that not the reason.



.... if you’re seeing that most of the nutritional value is used up in the first flush (resulting in a much smaller second flush), it’s consistent with the use of high-nutrient grains like rye or wheat berries.

For a more extended fruiting period, you might experiment with other grains and ensure optimal hydration between flushes.
Using millet or sorghum (these grains tend to sustain longer flush cycles).



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Re: Ask Quick Questions, Get Quick Answers [Re: Tri] * 1
    #29111997 -

Did translation machines evolve this much or are you really fluent in German? Just asking as someone who thought translation machines sucked too much ass to seriously consider (until now..?).

Edit: it is a translation. This thing (although I wouldn't use it as I hate AI) seems to bridge the gap between simply translating, one-for-one, every word (producing bogus) and translating the core intentions of the writer (which transposes meaning but renders the use of specific words or constructs useless). Impressive.


--------------------
No nation, no border
Fight law and order


Species grown so far

Edited by Churpee Choozle (02/03/25 01:30 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29112000 -

German is my second language, and if Tri's text was translated, this was a fair translation. If it is, this shit has evolved ever since my initial disappointment. I'd use this for a language I suck at (say, French).


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle] * 2
    #29112003 -

basz meg a kurva anyadat


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle]
    #29112007 -

Bump.
Any input? I really don't know what's happening here


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40] * 4
    #29112009 -

Edit: conjecture. Need coffee.

Catnip, are you visiting Ozora this year? I'd love to meet up. Will pack some prints. The bald guy in a cactus dress


--------------------
No nation, no border
Fight law and order


Species grown so far

Edited by Churpee Choozle (02/03/25 02:51 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LobstaBob]
    #29112019 -

LobstaBob said:
LobstaBob said:
Agree!

Hey guys, just a quick question, does this look like some funk?

Fresh as growers here, had success but minimal, apart from the tubs I have now.
Anyways I went millet gran spawn to coco cor on 1/19 first tub colonised nice and quick this one is about 2-3 days behind.

The strain is Jackiti (Jack Frost x shakiti?)
My question is WTF is this thing?? Looks like a cactus! 😂
And do u guys think my mycelium looks a lil whispy? Kinda like contam? 😭




Second pic I have posted is of the 1st tub I mention that was a few days advanced on the above pictured. I put into fruiting conditions last night.




Any thoughts?


Anyone seen anything like this? From previous feed back, some are suggesting my substrate could be too wet and too much build up of CO2 while colonisation? Some say it is contam some say it’s the start of pins (hard to believe)
Are these just stabs in the dark? Does anyone have any idea?

3rd pic is of a second tub I spawned to tub at the same time. Doin much better, which makes me think the lil cactus button is a contamination.
But what y’all reckon?

Edited by LobstaBob (02/03/25 01:56 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle] * 1
    #29112021 -

Churpee Choozle said:
German is my second language, and if Tri's text was translated, this was a fair translation. If it is, this shit has evolved ever since my initial disappointment. I'd use this for a language I suck at (say, French).



Yes it was a translation, how was it? Pretty coherent right?


--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Tri] * 1
    #29112072 -

Yeah it correctly conveyed your message. Stern but fair, the syntaxis and grammar are really good. For something out of steel and silicium


--------------------
No nation, no border
Fight law and order


Species grown so far

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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