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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted] * 1
    #29109437 -

:awehigh:

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9]
    #29109517 -

KlarnEvil9 said:
I have some of those gray lids, unmoved, that gave me some issues with my last jar run. I'm going to drill holes in mine and add filter disks and see if that helps.



Right there with you brother, filter disk on all my next jars. It's a far stretch, but a little lost at the moment and willing to try anything. Read that nektar61 had the same issues with unmodded an stipe'n cap use modded lids, gonna try it, cuz I'm lost at what fucked me. Thanks for sharing.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29109521 -

I really like the cheap plastic lids. They suck for unmodded, but work great with a filter disk RTV'd on. They're $21 CAD for 24 of them.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 2
    #29109528 -

PuffM4gicDr4gon said:
I really like the cheap plastic lids. They suck for unmodded, but work great with a filter disk RTV'd on. They're $21 CAD for 24 of them.



Yeah, but I'm in Norway, and everything is expensive. But that said, I have filter disks and going to try that.. It's like walking in the dark, I have no clue to why my tubs are fucked, but I'm going to get thru this and make it work..  Thanks tho, appreciate it.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 2
    #29109533 -

Smellyhobbit said:
Why do wood chips need to be fermented?



uncle Paul explains:


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“All this buttoning and unbuttoning”

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29109543 -

Kloakk said:
PuffM4gicDr4gon said:
I really like the cheap plastic lids. They suck for unmodded, but work great with a filter disk RTV'd on. They're $21 CAD for 24 of them.



Yeah, but I'm in Norway, and everything is expensive. But that said, I have filter disks and going to try that.. It's like walking in the dark, I have no clue to why my tubs are fucked, but I'm going to get thru this and make it work..  Thanks tho, appreciate it.



I'm in the same position as you. For the last year I've had trich show up almost every time. Before pins in the beginning, but now before first flush is done. The only good thing is that it has really helped me tighten up my process and learn new techniques.

I'm doing Cabin Sequestering and trenching to hopefully rule out/ eliminate bacteria and other hidden contaminants. My plates are always turning out tomentose, so I'm leaning towards that as a cause.

I'm also switching away from oats. Getting some Soft White Wheat and Millet tomorrow.

Not saying that is necessarily your cause, just sharing my experience.

Best of luck to you!


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 3
    #29109552 -

I think for unmodded lids you need to avoid touching or moving the jars as much as possible. You tighten the lid for the shake, unscrew it and then litfa until full colonization. I knew I wouldn’t be able to not touch and check them every day so I went with modded lids from the beginning :grin:

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon]
    #29109562 -

Have you tried changing agar recipes? I’m not saying this is your case, but I switched from brf agar to lme agar and my permanently tomentose cultures became pretty rhizos even at T1s in the new agar

Also do you have a flowhood or ffu? If you don’t, doing cabin sequestering and anything that implies having the plate exposed for longer and doing movements above it might cause some issues. If you are working only with a SAB, brf pucks with a 3-4mm trench are super useful to clean bacteria in my experience

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #29109588 -

GenesisCorrupted said:
To make pine liquor?
:shrug:



Methanol

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29109589 -

Kloakk said:
PuffM4gicDr4gon said:
I really like the cheap plastic lids. They suck for unmodded, but work great with a filter disk RTV'd on. They're $21 CAD for 24 of them.



Yeah, but I'm in Norway, and everything is expensive. But that said, I have filter disks and going to try that.. It's like walking in the dark, I have no clue to why my tubs are fucked, but I'm going to get thru this and make it work..  Thanks tho, appreciate it.



I also use the cheap plastic lids, I use filter stickers, I will start RTVing them on soon. But after I inoculate the jar I've started using 1" parafilm around the lid edge and jar to make as good of a seal as my plates get. That way if I get contamination, it's either my grain prep or my inoculation without a doubt.

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9]
    #29109600 -

KlarnEvil9 said:
Dumb Q: Can I use rye for Pans? I need to get my Pan cultures onto some grain soon, I have rye on hand. I know it may not be optimal, but is it a waste of time, effort, and grain?



Which do you think are the most optimal?

Q1. Yes

Q2. No

Edited by LewDoja (01/31/25 09:11 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Radamanto]
    #29109619 -

Radamanto said:
Have you tried changing agar recipes? I’m not saying this is your case, but I switched from brf agar to lme agar and my permanently tomentose cultures became pretty rhizos even at T1s in the new agar

Also do you have a flowhood or ffu? If you don’t, doing cabin sequestering and anything that implies having the plate exposed for longer and doing movements above it might cause some issues. If you are working only with a SAB, brf pucks with a 3-4mm trench are super useful to clean bacteria in my experience



Funny enough, I started the culture with a trenched BRF puck.

I currently use .5% lme.

I am using a SAB, so far only one ended up with a mold spore on it. I tossed that one. Here's hoping the rest are OK. I've been on the road for a week so I haven't checked them in a bit.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29109621 -

blueberrybroccoli said:
What is the current consenus/opinions about unmodified tubs and just flipping/adjusting the lid for dialing in? What are the pros and cons?

I see a lot of praise for pastywhites ez dialed monos, but i like to keep things as simple as possible.



no consensus, just opinions and preferences :shrug:
(you can see both in my signature)
these are the physics illustrated and you can infer what could go south with unmod:

the only inaccuracy of the video, IMO, is that the FAE holes should be uncovered from day 1, for speed of myc colonization and to not let anaerobic prone bacteria get a leg up.

"flipping/adjusting the lid for dialing in" the issue is  precisely on dialing in when the farmer lacks the sensibility to do so :biggrin:


--------------------
“All this buttoning and unbuttoning”

Edited by 999999999 (01/31/25 10:15 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: 999999999]
    #29109683 -

I've been working with some dirty spore plates and unfortunately some mold from them made its way into my sab and onto some other agar projects.

A lot of them ended up on plates that I have duplicates of so I'm just tossing those, but there are a couple that I would like to transfer away to a fresh plate. The mold has already sporulated in these plates

Besides taking a transfer from the furthest area from the mold spot, are there any other tricks I can use?

Would a BRF puck, trench, josex poke, or water agar help at all? If I'm remembering correctly those are methods for bacteria but I could be wrong.

Any suggestions are welcome.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon]
    #29109687 -

I believe you’d have a good chance at success if you just transfer from furthest away. Try that first at least imo


--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #29109703 -

Hi all,

I just did my first agar pour today! I did not inoculate them yet because I want to know if my pour tek was good. So I am leaving them uninoculated for a few days to see if any contamination occurs.

Where would you store them, the fridge or on a shelf room temperature somewhere? They are wrapped in parafilm. Would you also put them in a ziplock or something? The bag they came in got mangled by me, so I can not put them there.

Thank you for your input!


--------------------
:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #29109708 -

Stipe-n Cap said:
Big Jon said:
I’ve read Teks that suggest heat sealing bags for truffles making PRIOR to sterilization and I have read Teks that suggest leaving the bags open (not heat sealed) but still folded underneath the bag with a plate on top to prevent it clogging the vent.

What’s the difference between heat sealing the bags of grain prior to sterilization and NOT heat sealing the bags, if any,



No requirement whatsoever to pre-seal bags. This will explain in detail:






Thanks! He doesn’t mention anything about water & forked capacity, however. Is this video assuming that you have already pre-hydrated the grain?


--------------------
FYI: I am having uploading pics to Shroomery (says the file is too big, even with small screenshots) so any pics I post are linked to another site

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Re: Ask Quick Questions, Get Quick Answers [Re: Big Jon]
    #29109710 -

Evening all.

Any idea what this is? I am in fruiting conditions on a grow. Are these Pins or just really aggressive mycelium? never seen a ring formation like that.

Thanks




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Re: Ask Quick Questions, Get Quick Answers [Re: reggiecat] * 1
    #29109711 -

reggiecat said:
Hi all,

I just did my first agar pour today! I did not inoculate them yet because I want to know if my pour tek was good. So I am leaving them uninoculated for a few days to see if any contamination occurs.

Where would you store them, the fridge or on a shelf room temperature somewhere? They are wrapped in parafilm. Would you also put them in a ziplock or something? The bag they came in got mangled by me, so I can not put them there.

Thank you for your input!



I leave my plates in my SAB for the most part until I use them, otherwise a ziploc would be fine :thumbup:


--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29109717 -

LewDoja said:
KlarnEvil9 said:
Dumb Q: Can I use rye for Pans? I need to get my Pan cultures onto some grain soon, I have rye on hand. I know it may not be optimal, but is it a waste of time, effort, and grain?



Which do you think are the most optimal?

Q1. Yes

Q2. No



I think I have oats in my brain for pans, but that probably came from something outdated. I'll give 'er a go.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Shop: Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy


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