Lignicolous said:

I tried my hand at some tissue cultures of two radically different phenotypes of G. subspectabilis that I collected last season. I managed to get a couple of what appear (at least to my untrained eye) to be clean plates of one of them. I wasn't really on top of the transfers and all five attempts at the other one were overwhelmed by contamination. I've got some grain going now though and might try tossing some dowels on what's left in the good plates. One of the early plates that I kept around is pinning now, which is kinda fun.

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Smellyhobbit said:

Here we go. Not as much as I thought 15oz would be, but I bet it’ll do alright. Going to make some up and send a jar tonight.



Sent 2 tubs still of the same MS F0 that I’ve been working with.

These tubs are both 1:2 spawn:sub. Substrate is 1:1 coir and hydrated fir sawdust. As I type this I realize nowhere on the bag or website does it say “Douglas fir,” but I hope this works regardless. Poured boiling water over them until they stopped absorbing.

1 tub has a 1 quart straight coir top layer and one has no top layer at all. Now we wait.




Edit: I confirmed with the supplier that it is indeed douglas fir 👍

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The 1:1 sawdust:coir substrate without a coir top layer got cased today. Surface was forming little myc mounds, pseudosclerotia has formed, and I think we’re close to fruit.

Scemilope said:

When I started to doubt my plates would germ I stuffed a couple swabs into BRF pucks. Those have also germinated.

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Smellyhobbit said:

BRF is the best tool for stubborn spores. Love it for germing.

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I'm still having all kinds of germ trouble that I never have before.  I think it's just too cold in my house

Friend nearby was having similar problems until he build (I know) an incubator for them.