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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Peleus] * 3
    #29105379 -

Peleus said:
Is this one ready to be harvested?



Looks like a frilly hat from the Roaring 20s!


--------------------
“Order is needed by the ignorant but it takes a genius to master chaos.”
― Einstein


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Re: Ask Quick Questions, Get Quick Answers [Re: KauaiOrca]
    #29105394 -

Would wedge samples survive freezing conditions?


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29105407 -

DV8DUG said:
Is it possible to pull a monokaryon from a plate and bring it all the way to grain and have it look like a mated culture?

Or does it look dissimilar and can't be mistaken for dikaryon?


Not likely. Monos usually (read always) require a scope to suss out. They look different on a plate once grown, and then mostly won’t fruit if sent alone. You’d know if you had a mono.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29105409 -

Goal: I am trying to germinate swabs using brf pucks.

Method: I used a scalpel & tweezers to pull off a little tuft of cotton swab, then placed it on the puck. (I only have 1 swab of this variety, so I'm being cautious with how I use it. Can't put all my eggs in one basket).

Issue: I tried to push the swab down into the puck, but little bits of the puck would get stuck to the tweezers and it was difficult to get the swab off. I compromised by gently laying the swab tuft on top of the puck.

Timeline: I put swab > puck 10 days ago

Concern: I'm worried that the spores are not making contact with the food/water in the puck and cannot germinate. 10 days is not a long time, but if it keeps stretching out then I'd like to know how I can facilitate germination

Question: What would you do to get germination?

Question 2: Are these ideas silly?  Open jars in SAB, then...
  • Drop sterilite water on it from syringe
  • Drop lc broth on it from syringe
  • Transfer a small square of agar on top of it, like adding the roof in cabin sequestering


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MUSHROOMS RULE

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Re: Ask Quick Questions, Get Quick Answers [Re: Dala] * 2
    #29105412 -

I would drop sterile water onto the material to hydrate, yes.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Dala]
    #29105415 -

What about poking it deeper into the puck with a scalpel? Or using your scalpel to push a little brf over your swab and patting it down?


--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29105417 -

Huskies said:
Would wedge samples survive freezing conditions?


Some! I’ve heard of folks successfully reviving frozen cultures, but at a far less than 100% recovery rate. More like 50%, but that’s anecdotal and YMMV


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𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: gibilan11] * 3
    #29105418 -

gibilan11 said:
Would it be relatively safe to store PC'd rye jars for something like 2 weeks?

I waited too long and my agar culture reached the edges of the plates, so I think it's better to do one more agar transfer to be sure it's kept clean.

The lids are metal with rubber seal (previously used), with 4 small GE holes and tyvek fabric filter on the inside.

Should I seal the holes with to prevent the grain from drying or does that increase the risk of contam?


I’ve left mine for months with no issue at room temp, if they are sterile they are sterile.


--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Tri] * 1
    #29105446 -

When would you harvest these? They seem to finally be “growing out” of their fuzzy feet, but overall growth has stopped. Some of them are still fattening up a bit, but they’re not getting any taller. They’re getting more wrinkly and funky though

They’re still ROCK hard. Like surprisingly hard.

They’re Hillbilly Pumpkin X MelMac

Thanks for any advice!

(Ignore the sharpie marks. They’re for drilling holes for FAE during the time I take the sub out to harvest)



Edited by SidePinKing (01/28/25 03:38 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29105447 -

Is 70% isopropyl alcohol with moisturizer okay to use for mycology? Or should it have no moisturizer?.. which I have been using. The alcohol with moisturizer is a lot cheaper and I'm trying to save money


--------------------


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Re: Ask Quick Questions, Get Quick Answers [Re: Tri] * 1
    #29105452 -

Thanks for the input Smelly.

Tri said:
What about poking it deeper into the puck with a scalpel? Or using your scalpel to push a little brf over your swab and patting it down?


Initially I tried to poke it deeper, but the brf was sticky and got stuck to the tweezers, which also kept the swab stuck to the tweezers (and later the swab got stuck to the scalpel as I used it to get the swab off the tweezers lol).

However, scooping a bit of the puck over top of the swab is a good idea and i might use that approach next time.

Thanks y'all


--------------------
MUSHROOMS RULE

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 1
    #29105550 -

Jrey16 said:
Is 70% isopropyl alcohol with moisturizer okay to use for mycology? Or should it have no moisturizer?.. which I have been using. The alcohol with moisturizer is a lot cheaper and I'm trying to save money


For what application? You really don’t need 70% iso at all


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: SidePinKing]
    #29105557 -

SidePinKing said:
When would you harvest these? They seem to finally be “growing out” of their fuzzy feet, but overall growth has stopped. Some of them are still fattening up a bit, but they’re not getting any taller. They’re getting more wrinkly and funky though

They’re still ROCK hard. Like surprisingly hard.

They’re Hillbilly Pumpkin X MelMac

Thanks for any advice!

(Ignore the sharpie marks. They’re for drilling holes for FAE during the time I take the sub out to harvest)





Let them soften up like a Marshmallow


--------------------
The Hitchikers Guide to the Shroomery

Sometimes you just have to shut up and color!
~CMS Wilbur

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #29105567 -

Hello kind people of Shroomery!
My solution to most worries I've had has been patients, but now I've come so far, so now I'm here once again in search of clarification. I have a small tub with coco-coir with rye berries 4:1. Everything colonized really fast and now there are white dots everywhere (good right). There are some parts that are not white, but there is myc there, just haven't broken the surface (don't know if this is good or not). Now I'm starting to get pins, and I've heard that I need to increase FAE. Normally one just puts the lid upside down, but since I have these bags I'm a bit confused on what to do.

So my question is: do I need to give FAE (how much?)? A user here said that I could cut a slit 2 inch over the surface, I was thinking that I can open it up a bit on the top of the bag (so from the side the bag has the shape of the top of the number '1') so I don't need to cut it. The thing is that only half of the substrate has water beads on it, you can see where it glimmers and not in the pictures (the perspective makes it look like less than half has water, but it's like 50/50). Does this mean I need to mist the sides?

Also, about the parts where the myc hasn't broken through fully, can that cause problems when giving FAE? Does my sub even look healthy?




Sorry for bad quality.

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Re: Ask Quick Questions, Get Quick Answers [Re: OLDDAD77] * 1
    #29105581 -

Mwj12977 said:
Let them soften up like a Marshmallow


Heard, will do! Thank you :thumbup:

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Re: Ask Quick Questions, Get Quick Answers [Re: Dala] * 1
    #29105599 -

Dala said:
Thanks for the input Smelly.

Tri said:
What about poking it deeper into the puck with a scalpel? Or using your scalpel to push a little brf over your swab and patting it down?


Initially I tried to poke it deeper, but the brf was sticky and got stuck to the tweezers, which also kept the swab stuck to the tweezers (and later the swab got stuck to the scalpel as I used it to get the swab off the tweezers lol).

However, scooping a bit of the puck over top of the swab is a good idea and i might use that approach next time.

Thanks y'all


Just another thought, when I have a single swab and need to get the most out of it, like others have mentioned cutting the swab into petals (similar to what you did) I will streak at least 2 plates with it and bury some of it hopefully in more the one spot. And the buried cotton is usually the first place to have noticeable growth.

Also these petals are very tiny.

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Re: Ask Quick Questions, Get Quick Answers [Re: capsap]
    #29105607 -

So I'm confused, are you using tubs or bags?

Are they all in ones?

Was the rye colonized before going 4:1 with coir?

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29105641 -

LewDoja said:
Just another thought, when I have a single swab and need to get the most out of it, like others have mentioned cutting the swab into petals (similar to what you did) I will streak at least 2 plates with it and bury some of it hopefully in more the one spot. And the buried cotton is usually the first place to have noticeable growth.

Also these petals are very tiny.


Why? I got a swab from Nektar, I took my scalpel and scraped the swab and got plenty spores, the swab still look like when I got it.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29105689 -

LewDoja said:
when I have a single swab and need to get the most out of it



--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29105695 -

The x7x+ is a heavy sporer and can be massive prints. Not all swabs will be so easy to scrape spores off. Or maybe be more difficult to get germination to begin with (older).

Edited by LewDoja (01/28/25 06:58 PM)

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Shop: North Spore Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Grow Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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