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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29103426 -

Jrey16 said:
LyleChipperson said:
Jrey16 said:
LyleChipperson said:
I just had that happen with an agar batch too, you can still use them.


How was the growth?


No different than usual, even if there's less dissolved nutrients it should still be enough.


I kind of a perfectionist and the plates are bothering the fuck out of me lol. Even thinking of pouring more plates, but if these plates would work I'm not gonna waste my time.

I recently got a new pressure cooker, a Presto 23 Qt and it was my first time using it for agar. My old PC was a cheap one from China that was defective af. The pressure gauge wasn't working for example and I always had to just guess and hope it was on 15 PSI but I actually never had any problems with it lol. Idk if it was the way I PC'd the agar or if my LME is getting old because it is all rocked up and I live in a humid environment.


LME is hygroscopic and hydrophilic- keep as airtight as possible.
You might need to dissolve prior to PC, to ensure it doesn't just stay clumped on the bottom if media bottle. 

15-20min is sufficient @15psi

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29103452 -

LewDoja said:
15-20min is sufficient @15psi


Not to be too pedantic, but the 15 minute sterilization cycle is how long the *media* needs to be at that temperature, not the steam around the bottle. If you start with nearly boiling media, 20 minutes may be sufficient. (And with low biological loads, as may be expected with just malt extract and agar, you probably get away with it often even with cool bottles.) But in general, it takes some time for the media to get to temperature. Many references give 40 minutes as the safe time for 500 ml of liquid, 45 minutes for 1000 ml.


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29103465 -

Phaethon said:
I'd bet on the malt extract not fully dissolving. I always pressure cook my agar media for 45 minutes, sometimes longer if I'm not paying attention. If I have sediment it's because I used nutritional yeast and that shit never quite dissolves for me even when it's finely powdered first.

Do you boil and shake your bottles or jars before pressure cooking? It's not really required, but I find it makes it easier to ensure the malt sugar is dissolved, and getting the agar melted and uniformly distributed means it comes out of the PC nice and uniform instead of in two layers (higher concentration of agar on the bottom). I just put all the ingredients in cold and microwave with the lid very loose until it starts to boil.



I didn't boil but I did shake the fuck out of it since it was all rocked up. Where I live is really humid and I heard LME goes bad faster and rocks up in humid areas. I had that batch of LME for about 6 months now. I also PC for 45 minutes and I usually do 900ml water. And I have no microwaves. Most households here don't have a microwave haha


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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29103469 -

Yup I keep it in an airtight jar. Luckily I have some new LME which I'll just use that now. I'm thinking of just making a new batch.. but such a waste. But if these agar plates I just made will work just fine I might as well not. But I feel like most of the nutrients are stuck on the bottom.


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 1
    #29103472 -

Have you tried storing the malt extract with a silica gel desiccant in the jar? If you have a vacuum sealer with a jar attachment, that could also be worth trying - if the jar is really airtight, then evacuating most of the moist air should prevent it bricking up.


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon]
    #29103474 -

Phaethon said:
...If you start with nearly boiling media, 20 minutes may be sufficient.



As long as a proper venting was done it should be.  I also  gradually bring up to temp vs balls to the walls (medium high vs high).

But you're correct it's success is more based on having minimal biological load.

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 1
    #29103475 -

I think they'll be fine. At least wait to see if it fails, before making new.

Water Agar works, so even if there isn't as much nutrients uniformly dispersed it should still preform.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29103483 -

Just streaked my first plates ever the night before last.

I didn't realize the agar was so delicate and my loop pierces it very easily. 

So I was a bit heavy anded a a few and dug I to service a little bit.  live and learn I guess. 

Are they gonna be ok?







--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29103485 -

They'll still work, sometimes mine is like a cheese slicer.

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29103490 -

LewDoja said:
They'll still work, sometimes mine is like a cheese slicer.



Cool cool.

Oh and Thanks for the culture btw


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16] * 2
    #29103496 -

Jrey16 said:
Are these plates still good to use?

I just got done pouring some agar and I noticed what I think is pieces of light malt extract on the bottom of the plates. Some of the light malt extract I used was all rocked up and I'm guessing it didn't fully dissolve. I used LMEA and pressure cooked it for 45 minutes at 15 PSI.






Nothing to worry about, it's just malt protein, your agar is still a homogeneous solution. :naughty:


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29103797 -

Any ideas what these splits towards the caps are? HBP cross with PE I think?



--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 1
    #29103806 -

Is this a good starter scope for culting?




--------------------
This hobby has taught me many things.
Most of those things are patience.

The Hitchhiker's Guide to the Shroomery
The Official Shroomery Cultivation Museum
CakeWars
III

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29103858 -

Most of my spore plates look like that, it's fine. I like to think the cuts in the agar provide more surface area* to make contact with the spores!

*probably not true, but it sounds good.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: Scemilope] * 1
    #29103921 -

Scemilope said:
Is this a good starter scope for culting?





probably not.  To see bacteria or fungus, you need a microscope with at least 400x magnification. However, you'll likely need a higher magnification of 1000x or more to study structures and identify species.

quick and dirty AmazonFu yields this https://www.amazon.com/Omano-Microscope-Full-Size-Professional-Laboratory/dp/B006I6XCY6

But as always with optics, I think you really need to spend at least $300-$400 for quality and clarity.  Similar to binoculars.

Edited by Jebi (01/27/25 12:45 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Scemilope] * 1
    #29103923 -

Not really, maybe for macroscopic features... dissecting, inspecting, or working with larger specimens.

Amscope B120 (400x–1000x) is good though... to study spores or perform advanced fungal microscopy, you’ll need a compound microscope.

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Re: Ask Quick Questions, Get Quick Answers [Re: Scemilope] * 3
    #29103926 -

Depends on what you want to do. If you intend to count clamps, or make detailed drawings of cheilocystidia, this won't make the nut. You'll want at least 1000x nagnification for that.

For day-to-day questions like 'did my spores germ?' or 'is that itty witty thingy bacterial goop or just some wrinkle on the surface of the agar?' this will work great. At 60x you can follow each hypha individually and spot contams before the regular old naked eye is able to.

I am personally really happy with a scope that is basically the same.

Edit: as per usual, I was slow and others chimed in. I had a 1200x scope and virtually never used it. Now that I have like an 80 or 100x, I actually use it, because it allows me to follow my streaks on a plate and see if anything's going on.


--------------------
No nation, no border
Fight law and order


Species grown so far

Edited by Churpee Choozle (01/27/25 12:48 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29103928 -

That tub gives me bacterial vibes. But I'm not sure that is the cause...

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle] * 6
    #29103930 -

Back in late as usual, but for the people debating wood pellets for cubes.... It's been done to death.  Yes, they will fruit on them.  No, it is not beneficial in any way whatsoever compared to coir.  You will need to sterilize or pasteurize it, monitor it closely for contamination, and enjoy a sub-par harvest.

Cubensis will grow on wood, just like if you stuff grain in the pages of a bible it will fruit cubes.  Doesn't mean they eatin' the good word.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] * 1
    #29103964 -

Piggy, how are you?


--------------------
No nation, no border
Fight law and order


Species grown so far

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