Axman said:

I would like to get mine sequenced, but have no idea where to even start with that. Can I just mail some spores somewhere or does it need to be fresh tissue? Ive done some reading on it and I'm still lost.

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Alan Rockefeller said:

Definitely save the collection, well dried at low temperature and labeled with the iNaturalist observation number.  If you want to send me a little piece of a cap I can have it DNA barcoded.  You should carefully save the rest of the collection in case it turns out to be a new species.

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Not sure if the same invitation/openness would apply to a wild cube, but it could be worthwhile to ask him! He recently shared the article from this thread's OP here, which makes me think he may find both Tico and this thread interesting

eilauver said:

Huskies said:

I understand that wild genes might have a bunch of variables in potency/yield that make them discouraging compared to a commercial one, but I'm very interested in seeing what happens when that gene pool gets revitalized. Maybe its best of both worlds?

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I feel like the goal of genetic variety and cultivation just not might go hand in hand. Even if one were to find something interesting in a much wider gene pool, for cultivation purposes it would make sense to then still select towards it.

One thing that I immediately thought were wild apple trees. Large majority of the time they are significantly worse than tasty and large commercial cultivars and I can't imagine lots of effort going towards finding something novel from them. Though I admit, it's apples and oranges, considering the length of time apples are domesticated vs mushrooms.

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3.A.M said:

I’ve sent out samples to Alan and also to Caine Barlow here in Australia, both just asked for pieces of cap and stipe dried at a low temperature (I went for 60c) which is what I dry my pans at, also have other friends involved in sequencing and they’ve just requested plates, another guy here in Aus is researching genetic links between cubes and I’ve sent him agar wedges in ziplocks, as long as there’s enough to work with and no cell damage you’ll be fine, not sure if freeze drying would be a good idea though.

As far as crossing I’ve kinda come to the conclusion you’d need to stabilise a little too, simply because most wild clones survived in the wild and managed to fruit in those conditions, give them a high nutrient food source and perfect conditions and they don’t seem to know what to do with it, same with F1’s, majority of those grows are actually pretty shitty, there’s always exceptions though, I’m sure if you crossed F1’s you’d get some very cool mutations happy to grow in tubs. But you’d also get a lot of trash.
My first and only cross ever to fail was a wild F1 x APE, obviously 1 attempt is nothing to go by and I’ll try again and see what happens but it took the wind out of me for the time being.
This is a standard clone grow

Not the most satisfying.
Got one pinning pretty nicely at the moment from this guy

Very curious as to whether it’ll repeat those ochra like stipes.

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Huskies said:

Very nice! Got any big flush pics?

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They haven't really flushed for me, I'm assuming its from the multispore, or that none of the spawn was 100% clean, but they're not all maturing at the same time. I'm looking forward to see the F-2 grows.

Axman said:

I decided to do a swab giveaway with my Tico genetics if anyone is interested.

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very cool write up about Tico, thorough! And to see it go from wild through those steps to fruiting is awesome

Huskies said:

Has anyone found unique phenotypes so far? Axman, I saw the gnarled fruit with wavy cap in the marketplace and it was very pretty. Did you clone that one by any chance?

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GreeneMyco said:

And cool on the giveaway Ax! Not quite of "age" to check it out just yet, but I might look back in ~2 weeks. Definitely interested And echoing Huskies, real neat cluster there!

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Huskies said:

Nice! Axman, thats a nice cluster you got.

3AM: I'm guessing the clones are always providing a better flush compared to non-cloned grows?

Has anyone found unique phenotypes so far? Axman, I saw the gnarled fruit with wavy cap in the marketplace and it was very pretty. Did you clone that one by any chance?

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In my experience both clones and F1’s provide pretty average flushes, the one above is an outlier, as far as unique phenos, I wouldn’t say it’s unique necessarily but


Definitely hefty