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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 2
    #29097636 -

Kloakk said:
KlarnEvil9 said:
Definitely try to clone it! I would harvest it, give it a rinse or wipe to remove excess coir, put it into your SAB, then split it lengthwise by tearing with your hands. Then you can scrape out a bit of inner stipe tissue (with a scalpel or forceps) from each half to go onto its own agar plate!


Sounds like a plan, thanks. Just rinse in tap water?


Should be ok, but I have seen people say to use peroxide or isopropyl to clean off the outside of the stipe as well. I've tried all 3, but with limited success (outdoor fruits are dirty!)


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] * 5
    #29097660 -

Unless it happens to be dripping with mud, I keep it dry. My thought is a wet outer surface can carry contams to the inside. I guess you could rinse and let dry, but I only do that with seriously dirty specimens.

Here's the last one that got rinsed, wild foraged.



--------------------
It takes guts to be
gentle and kind

Another way to live

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 4
    #29097689 -

TL;DR: Always vent…

Regarding venting, I’d like to add that I have an autoclave designed for submerging jars rather than steam sterilization (though I can run it like any pressure cooker as well). This allows it to operate at very high pressure.

If I run it without venting at 250°F/121°C, the pressure exceeds 3.5 bar, which is 50 psi.
When I vent it properly, it operates at 17–20 psi.

There’s really nothing to debate. Neither the Presto nor the All-Americans run at such high pressure, and you simply won’t reach 250°F without venting.

If air/steam keeps escaping with the weight on, eventually it’ll release all the air and retain only steam. However, in that case, you would need to start the timer at that point or extend the total sterilization time.


Edit: promoting not venting is not pseudoscience, it’s straight up misleading nonsense. I’ve got my fingers crossed that anyone being tempted not to vent, does so anyways for the sake of not taking shortcuts. Don’t let this be the reason for failure.

I’ll see if I post something in regards to pressure/temps with vent/no-vent after I’ve serviced the autoclave in the future.

Just vent the damn thing….


--------------------
= Pressure needed to reach 121C/250F, should you decide not to vent =
= Cabin Sequestering FAQ (to roof, or not to roof?) =

"Assumption is the mother of all fuckups"

Edited by MycCycle (01/22/25 03:23 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 2
    #29097709 -

KlarnEvil9 said:
Should be ok, but I have seen people say to use peroxide or isopropyl to clean off the outside of the stipe as well. I've tried all 3, but with limited success (outdoor fruits are dirty!)



LadysKnight said:
Unless it happens to be dripping with mud, I keep it dry. My thought is a wet outer surface can carry contams to the inside. I guess you could rinse and let dry, but I only do that with seriously dirty specimens.

Here's the last one that got rinsed, wild foraged.




I'll do a quick rinse and let it dry up, split and get some tissue, appreciate the help :clone:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Stipe-n Cap]
    #29097786 -

Interesting point about AA vs presto. As I run presto and have only just now gotten an AA. I've never had trapped air issues. I can't speak on AA

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Re: Ask Quick Questions, Get Quick Answers [Re: PhenoDreamers] * 1
    #29097788 -

I just remembered that you can get a tiny sample from the cleaned stipe using a sterile needle/syringe, sort of a Josex poke. Just poke the needle through and you'll have enough live tissue for it to grow.

I will try to find the post, but I think they used a little puff of air to get the biopsy tissue from the needle to the agar.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 4
    #29097796 -

Kloakk said:
I'll do a quick rinse and let it dry up, split and get some tissue, appreciate the help :clone:


Maaan that stipe must have been like 2mm, if I get growth from this then I already know I can clone pans:rofl:

Clone 1



Clone 2



--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] * 2
    #29097805 -

KlarnEvil9 said:
I just remembered that you can get a tiny sample from the cleaned stipe using a sterile needle/syringe, sort of a Josex poke. Just poke the needle through and you'll have enough live tissue for it to grow.

I will try to find the post, but I think they used a little puff of air to get the biopsy tissue from the needle to the agar.


I’ve used the Josex Poke successfully several times, I s up sterile water and peel open the stipe, poke it with the needle and used the sterile water to push it out, being careful not to squirt too much water on the plates


--------------------
Beef’s The Hitchikers Guide to Shroomery
Stipe’s Clean Spawn Checklist
Gummies by milkboy
Eat’s Easy Oats Prep
DV8DUG’s Recognizing Contamination in Grain Jars

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] * 2
    #29097811 -

KlarnEvil9 said:
I just remembered that you can get a tiny sample from the cleaned stipe using a sterile needle/syringe, sort of a Josex poke. Just poke the needle through and you'll have enough live tissue for it to grow.

I will try to find the post, but I think they used a little puff of air to get the biopsy tissue from the needle to the agar.


Water 👍

It’s literally a Josex poke, just on tissue instead of agar


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] * 1
    #29097816 -

KlarnEvil9 said:
I just remembered that you can get a tiny sample from the cleaned stipe using a sterile needle/syringe, sort of a Josex poke. Just poke the needle through and you'll have enough live tissue for it to grow.

I will try to find the post, but I think they used a little puff of air to get the biopsy tissue from the needle to the agar.


I read that thread, but as I don't have any needles at the moment I tried my best with what I had, it'll probably fail one way or another, but I hade to give it a go before it was to late, thanks again.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #29097817 -

Smellyhobbit said:
KlarnEvil9 said:
I just remembered that you can get a tiny sample from the cleaned stipe using a sterile needle/syringe, sort of a Josex poke. Just poke the needle through and you'll have enough live tissue for it to grow.

I will try to find the post, but I think they used a little puff of air to get the biopsy tissue from the needle to the agar.


Water 👍

It’s literally a Josex poke, just on tissue instead of agar


Word, I have used that with pretty good success, but I was going to BRF pucks, so the wee bit o' water wasn't an issue. But I could swear I remember reading another post about doing the poke with a dry needle/syringe for the purpose of cloning outdoor / wild fruits. Can't recall if it was going to agar or BRF.

Or I might be completely out of my mind. :shrug:


save me smellyhobbit, you're my only hope!


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29097818 -

:hellyeah:  Looks like you had success with your clones, that's awesome!


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] * 1
    #29097845 -

KlarnEvil9 said:
Smellyhobbit said:
KlarnEvil9 said:
I just remembered that you can get a tiny sample from the cleaned stipe using a sterile needle/syringe, sort of a Josex poke. Just poke the needle through and you'll have enough live tissue for it to grow.

I will try to find the post, but I think they used a little puff of air to get the biopsy tissue from the needle to the agar.


Water 👍

It’s literally a Josex poke, just on tissue instead of agar


Word, I have used that with pretty good success, but I was going to BRF pucks, so the wee bit o' water wasn't an issue. But I could swear I remember reading another post about doing the poke with a dry needle/syringe for the purpose of cloning outdoor / wild fruits. Can't recall if it was going to agar or BRF.

Or I might be completely out of my mind. :shrug:


save me smellyhobbit, you're my only hope!


This?
https://www.shroomery.org/forums/showflat.php/Number/28406466#28406466


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] * 3
    #29097854 -

I can't tell if the clone biopsy discussion involves poking the exposed, outer flesh or if y'all are splitting the stipe, but I would think the latter is a safer approach.

Edit: It looks like that was the plan from the start. I apologize for being repetitive - I think I missed that whole page of the thread. 😬


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

Edited by ThurmanMurman (01/22/25 04:47 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29097881 -

That's not the same post, but it's a very similar idea. Although I think the one I remember was poking the needle across the stipe, which leaves the outer surface as a vector for contam.

I think Thurmann is right about collecting the biopsy from the inner tissue, far less possible contams.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 2
    #29097884 -

ThurmanMurman said:
I can't tell if the clone biopsy discussion involves poking the exposed, outer flesh or if y'all are splitting the stipe, but I would think the latter is a safer approach.


I’ve always split to stipe working in front of FFU


--------------------
Beef’s The Hitchikers Guide to Shroomery
Stipe’s Clean Spawn Checklist
Gummies by milkboy
Eat’s Easy Oats Prep
DV8DUG’s Recognizing Contamination in Grain Jars

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Re: Ask Quick Questions, Get Quick Answers [Re: Headass] * 1
    #29097906 -

I missed the part where Kloakk mentioned splitting the stipe. That was my bad.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

Edited by ThurmanMurman (01/22/25 04:57 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] * 1
    #29097912 -

KlarnEvil9 said:
That's not the same post, but it's a very similar idea. Although I think the one I remember was poking the needle across the stipe, which leaves the outer surface as a vector for contam.

I think Thurmann is right about collecting the biopsy from the inner tissue, far less possible contams.


Ah I see, can't remember seeing that thread in my search. And if the stipe wasn't this tiny little thing and I had better tools I would take a different and better approach, like Thurman and Headass is saying. I did try to get inner tissue, but I had to use my scalpel to split it and to get a sample, probably got some of the outer tissue to. We'll see in a couple of days I guess. :biggrin:

Speaking of cloning small fruits, how are the pan growers doing it? I am dreaming of getting a hold of a TTBVI print, and after reading baba's pan monotub thread he is saying the right genetics is key, but I can't remember anything about how he is cloning :strokebeard:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29097916 -

QQ: What should I put on the table that has my SAB on it to prevent slipping? It's a folding plastic table, pretty slick on top without a towel or something.

I know that a bath towel is probably not the way, but I'm not sure what I should be looking for. A different type of towel? Or some adhesive rubber gasketing around the lip that makes contact with the table?

Current SAB pic:


She's a sight better than the soft sided one I had (I know I suck), but I don't know if the towel is causing problems or helping solve them.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29097920 -

Kloakk said:
KlarnEvil9 said:
That's not the same post, but it's a very similar idea. Although I think the one I remember was poking the needle across the stipe, which leaves the outer surface as a vector for contam.

I think Thurmann is right about collecting the biopsy from the inner tissue, far less possible contams.


Ah I see, can't remember seeing that thread in my search. And if the stipe wasn't this tiny little thing and I had better tools I would take a different and better approach, like Thurman and Headass is saying. I did try to get inner tissue, but I had to use my scalpel to split it and to get a sample, probably got some of the outer tissue to. We'll see in a couple of days I guess. :biggrin:

Speaking of cloning small fruits, how are the pan growers doing it? I am dreaming of getting a hold of a TTBVI print, and after reading baba's pan monotub thread he is saying the right genetics is key, but I can't remember anything about how he is cloning :strokebeard:


Your sample plates looked good, you managed to get a nice, tiny specimen. Well done!

As far as cloning small fruits goes, Josex poke all the way! I think they recommend using a 21ga needle to get the smallest sample possible. But I know pan growers have used this since it's so precise and designed for tiny shrooms.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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