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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29092676 -

I do too. At least if masking tape = the tape you use when painting stuff and protecting the not-to-paint parts with (?). And I use that tape all the time for containers with Oyster in it. There is enough gas exchange possible to make the Oysters find their way. And it is much more pleasant to rip the masking tape of, instead of the peeling of off masking tape. Also way more pleasant to the wallet. At least where I live, micropore tape is quite expensive.

It seems like the cheap masking tape I use, is also useable to do PC work with. Because recently I accidentally PC-ed a big jar (1.5 liter) of grain, with 4 holes on the side covered with masking tape. I didn't get any (noticeable) contamination. My rationale says it is due to luck, and
not to an intrinsic characteristic of this masking tape. For fun I will do a full batch of grain jars soon with this specific masking tape instead of micropore tape. And see what happens. Can't do much with this, as I don't think the production process of this tape is consistent. I wouldn't ever use it as masking tape anyways; it's the cheap shitty stuff with all paint problems that come with it.

If you're wondering: basically that 'accident' happened because I was stacking multiple big jars with grains to PC. And one of my jars to hold substrate + spawn, I accidentally used for the grain. I was not paying attention for some reason. I am trying to get away from plastic containers to glass substrate holders. But in the mean time I got caught in the use of plastic bags. And actually very much like the no-need to wash. Pff. Long text. Sorry!

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork]
    #29092700 -

I'm new to bags and trying out some stuff with PE Uncut in them. Finally a bag with spawn and coir starts to fruit. A bit... I don't have a fruiting room. Only normal living rooms available with +-19 Celcius and humidity ranging from 40-80%. I went a bit on the edge here regarding ratios btw: 0.5l spawn on 3l coir.

Is the right approach to let these bags top fruit by poking holes in the bag?

And what about making a cut in the side?

Can anyone point me out to some info that better gets me on track?


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 6
    #29092729 -

callmehippie said:
Maby i misinterpreted.  Here  RR says this



Here's the link to the thread.

https://www.shroomery.org/forums/showflat.php/Number/19406513/fpart/all



RR was a monster of shroom advancement a decade or two ago. He started on this site in 2003, and figured out how to grow way before many people even ate a shroom, and before some people here were born.

We're all just standing on the shoulders of giants here.

But some of his teks and advice are way out of date, wrong, or just not done anymore in favor of other better teks.

Also, he was such an expert that if he guessed "letting spores drop messes up your grow", people would take it as gospel.

For that matter, is anyone still using fucking rattlesnake venom to cross two cubes, (or did it ever even work to cross two species?) like Roger and others did back when, or are we just rubbing spore swabs together like Fhat now.

Venom for cube crossing sounds like voodoo at worst and unneeded at best, but when I first came on here, I was convinced I needed some, based on old posts.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (01/18/25 04:20 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] * 7
    #29092739 -

It doesn’t really even make sense from an evolutionary standpoint.  Mushrooms flush multiple times.  They haven’t reached the end of their life-cycle

He probably had a good flush and had a tub go too long a couple times and it was spent so he mixed up correlation with causation.. happened a lot back then.  I may have been guilty of doing that a time or two :lol:

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Re: Ask Quick Questions, Get Quick Answers [Re: fahtster]
    #29092765 -

Can anyone recommend or link a good brand of 50ml self-standing centrifuge tubes with proper caps that will withstand multiple autoclave cycles? Glass or pp5 is fine around that size, as long as the lids won't deform or melt.

I plan on modding the caps, by drilling a vent and adding small filter pads. So I can use them as plant tissue culture seed rescue/starting vessels. I also wanna be able to sterilize the TC media right in the tube, so basically doing no pours.

Does it really matter what material the cap is, if I plan on venting it that way anyway? Or will they just deform quickly?

All the brands I see listed on amazon selling bulk packs are misleading about the material of the cap. Most don't even say what it is, just that its "leak proof", etc. I can't find any that specifically use pp5 for the caps too. Only the tubes, but many ads makes it seem like the cap is pp5 too. Some of the ones that say you can autoclave (up to 120C) claim to use PE plastic caps though, so I don't really trust it.. Do they mean with or without the lid on? :confused:


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Re: Ask Quick Questions, Get Quick Answers [Re: cybork]
    #29092787 -

cybork said:
I'm new to bags and trying out some stuff with PE Uncut in them. Finally a bag with spawn and coir starts to fruit. A bit... I don't have a fruiting room. Only normal living rooms available with +-19 Celcius and humidity ranging from 40-80%. I went a bit on the edge here regarding ratios btw: 0.5l spawn on 3l coir.

Is the right approach to let these bags top fruit by poking holes in the bag?

And what about making a cut in the side?

Can anyone point me out to some info that better gets me on track?





Surprised Faht passed this one by, try this, same thing really.

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Re: Ask Quick Questions, Get Quick Answers [Re: PsillySeeEms] * 1
    #29092789 -

PsillySeeEms said:
Can anyone recommend or link a good brand of 50ml self-standing centrifuge tubes with proper caps that will withstand multiple autoclave cycles? Glass or pp5 is fine around that size, as long as the lids won't deform or melt.

I plan on modding the caps, by drilling a vent and adding small filter pads. So I can use them as plant tissue culture seed rescue/starting vessels. I also wanna be able to sterilize the TC media right in the tube, so basically doing no pours.

Does it really matter what material the cap is, if I plan on venting it that way anyway? Or will they just deform quickly?

All the brands I see listed on amazon selling bulk packs are misleading about the material of the cap. Most don't even say what it is, just that its "leak proof", etc. I can't find any that specifically use pp5 for the caps too. Only the tubes, but many ads makes it seem like the cap is pp5 too. Some of the ones that say you can autoclave (up to 120C) claim to use PE plastic caps though, so I don't really trust it.. Do they mean with or without the lid on? :confused:





Most medical supply companies sell these and they have to be pp5 or autoclavable, not much price difference compared to Amazon either but I’m not in your country, shop around.
Fuck Amazon.

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29092792 -

BeefSupremeJr said:
youre good.  i think recognizing correct surface conditions is going to be  more critical than what kind of tape you use at this point.

also, do not snap that lid tight.  you need SOME air exchange.  maybe even open up a few hole.

creating a high humidity high co2 environment is a certain recipe for bacteria to run rampant or worse, trich. 

fahts taught me youre better off with lots of fae from the get go for this reason.  mist if you need to, but never choke it off.



:takingnotes: Better to mist, then choking them out, got it.

So now, I'm back to freeze tape again and I put it on 2 out of 3 holes on the long side and 3 out of 5 on the short side. I'll leave it like this for a couple of days and see if any condensation will appear.

Should I mist the walls of the tub to see at what rate it evaporates? Any other advice?

And also, I can't post pictures and get the message: "out of space", anyone got a fix for that? Fixed:awesomenod:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


Edited by Kloakk (01/18/25 12:57 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: 3.A.M]
    #29092849 -

3.A.M said:
cybork said:
I'm new to bags and trying out some stuff with PE Uncut in them. Finally a bag with spawn and coir starts to fruit. A bit... I don't have a fruiting room. Only normal living rooms available with +-19 Celcius and humidity ranging from 40-80%. I went a bit on the edge here regarding ratios btw: 0.5l spawn on 3l coir.

Is the right approach to let these bags top fruit by poking holes in the bag?

And what about making a cut in the side?

Can anyone point me out to some info that better gets me on track?





Surprised Faht passed this one by, try this, same thing really.



Thanks! So should be good now. Maybe to be added a bit more holes. Which I'll add today.

Anyone havind experience with making a cut on the side with cubes? I'm running that situation now also, but maybe someone could already get me out of an incorrect fantasy... :smile:

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork] * 1
    #29092928 -

cybork said:
3.A.M said:
cybork said:
I'm new to bags and trying out some stuff with PE Uncut in them. Finally a bag with spawn and coir starts to fruit. A bit... I don't have a fruiting room. Only normal living rooms available with +-19 Celcius and humidity ranging from 40-80%. I went a bit on the edge here regarding ratios btw: 0.5l spawn on 3l coir.

Is the right approach to let these bags top fruit by poking holes in the bag?

And what about making a cut in the side?

Can anyone point me out to some info that better gets me on track?





Surprised Faht passed this one by, try this, same thing really.



Thanks! So should be good now. Maybe to be added a bit more holes. Which I'll add today.

Anyone havind experience with making a cut on the side with cubes? I'm running that situation now also, but maybe someone could already get me out of an incorrect fantasy... :smile:



I also made an attempt to grow in bags now to do smaller grows and more variety instead of always running a whole tub.

I had a lot of side pins and blobs (PE and ESS). Im guessing it would have helped to compress the surface mor but i used very tall bags and that made it a bit difficult. Also after i added a casing ayer i couldnt really compress and level it out like in a tub. I would try putting an elastic band around the bag just under the fruiting surface or tape around tightly to help prevent sidepins. I initially just opened the top of the bags and gave them too little FAE which just dried them out i think. I then drowned the sub in water for some hours, added some generous holes & slits in the bottom and then got some nice pins finally. Definetely had a hard time though getting the conditions right. Will try again with ziplocks like in Fahts TEK. Just had these bags lying around and thought they look like theyre made for this.. turns out the filters really didnt allow much FAE
ESS          -          PE




--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: tittlez]
    #29092989 -

I want to do a small batch test of PF Tek cakes. Was wondering how long you would PC them in an instant pot.



--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29093039 -

I do 2 hours in IP for PF jars.

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Re: Ask Quick Questions, Get Quick Answers [Re: Jebi]
    #29093062 -





21 days after grain to spawn with SFF shoebox tek.
Is it normal to have little puddles like that?
I kept it close and latched the whole time. Should I supply it with FAE? because the moister seems really high

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29093070 -

Quote:
LewDoja said:
Do you get a bunch of water pooling in the agar plate?




Yeah I used to get some condensation. But now I place my plates in on the 3rd/4th level (cooler temps) facing up so a tiny bit of condensation forms on the lid. Then after they've sat for several hours I'll flip them and that gets rid of that condensation on the lid and water doesn't form on the agar after this preheating step.

Quote:
LewDoja said:
80 is fine for vegetative growth but remember the inside of jars will be warmer than ambient temp.

Also the higher the temp the increased potential for any unwanted organisms gaining a foothold.



Noted. Thanks for sharing :smile:

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Re: Ask Quick Questions, Get Quick Answers [Re: killedSanta] * 1
    #29093074 -

I'm very new here so take everything I say with that in mind.

I don't think you should have pools of water on your surface based on what I've seen in this post Proper Surface Moisture / When to Increase FAE.

I also think based on what I've seen discussed here and in that post you should have FAE from the start. I don't think you want those large droplets on the lid because those will drop and cause puddles, but someone with more experience will probably have some advice.

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Re: Ask Quick Questions, Get Quick Answers [Re: killedSanta] * 1
    #29093087 -

killedSanta said:




21 days after grain to spawn with SFF shoebox tek.
Is it normal to have little puddles like that?
I kept it close and latched the whole time. Should I supply it with FAE? because the moister seems really high



This will not be a quick answer, pard. bear with me.

So this is a good example of what zero fae does to a sub.  This should have fruited a long time ago.  At this point, water has matted the myc completely flat and there is very little possibility for this surface to pin.  It seems like there were a lot of things leading up to this that were correct, then at the fruiting state we kinda missed some critical information.

I'd read Comebackkid's writeup on surface conditions first. That ship has kindof sailed.
Theres unmodified tubs  but this involves a loose lid that allows for air exchange.  Yours appears to be one with a gasket and was snapped down snug. We call this 'choking'.  This thing choked.

Can we salvage it? Perhaps. I know others will tell you to dump it and start over but I tend to think you should always try to salvage what you can.
We'll need a brand new surface first.  What would I do? We'll being that you don't have anything else to fall back on, and you can't break whats broke, Id blast that surface off with the dish sprayer if you can.  It looks pretty solidified. If you have a removable shower head, you might have to try that out? I just went through this with another guy in another thread you might check it out.  Im not sure how he got his matted surface off I think he used kitchen utensils or something.

next, he kind of rolled his cake in some field capacity coir and set it in a watertub of his own making.  Totally unconventional but it actually looks like he'll get a new surface and might actually pull some shrooms. I dont think you'd need a water tub really, just a fresh layer of damp coir on top should give you enough moisture.

IN THE MEANWHILE, and what both of you should do, is start from the beginning of research to make sure you're not missing other critical elements. Pay CLOSE ATTENTION to comebackkids write-up on surface conditions as that will determine whether or not you are able to salvage anything from this project. Read the Hitchhikers guide linked in my signature from beginning to end and if you're unclear on anything read it again and then ask questions.  Choose your path if you want to continue this hobby and I'm happy to help you get wherever you want to get.

Good luck pardner.


At a second look, it kindof looks like there might be some pins forming.  Don't be shy.  Take the lid off and show us a good high quality picture of that surface please.  You might be able to get it to fruit and bypass that whole torture and punishment thing i said earlier. I can usually tell by these kinds of pictures that you were worried to open your tub for fear of contamination  which would indicate that you're getting information from reddit? I could be wrong.

If you look at my photo gallery, you'll see that I have no fear of opening lids. Youll see i have a bunch of holes in my tubs with no filter at all allowing normal airflow in and out of the tub at all times from the moment i spawn until the day I harvest and dump the sub. You see all that condensation on your lid? Yeah thats basically just raining back down on your substrate and matting.  You always want to take the lid off and shake those big droplets off once in a while if you're getting them.  Ideally, you don't get much rain until well into the second harvest in my experience, (if your fae is correct)


Edited by BeefExtreme (01/18/25 12:40 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29093121 -

So harvested a couple of “heads” of lions mane off this block.  It still has some small fuzzy bits (see pic). What should I do now?  Continue to nurture those little spots and see what happens?  Or somehow break it up and recolonize it in something.  I can always get a decent first flush of mushrooms but I completely fail at round 2.  Regardless of the method (block, 5 gallon bucket, etc)

Edited by Lestouffer (01/18/25 01:04 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29093132 -

Not my picture but wondering what causes the caps to get like that? Is it mycelium? And what's up with the very fluffy mycelium on the substrate? This is Tidal Wave




--------------------


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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29093157 -



Ok, so this tub was a clone of a mutant whose cap grew inside out. Im not seeing that expressing here, but I like those two tall bois in the back left. At this point, I would just be cloning a clone and instead, need to take a print from one of these, yes?


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
    #29093167 -

3 of my brf tek jars have started fruiting. Should I scrape the uncolonized substrate off and birth them at this stage or should I let it finish colonizing before I birth them? And what do I do with the fruits, harvest or leave them on while dunking?






--------------------

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