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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Let's All Grow Mushrooms 2025 [Re: Axman] * 7
    #29086194 -

Axman said:
1-12-2025:


Took transfers from each of the T1 plates, one to Charcoal agar and one to BRF agar each.

From each of the T1 plates I cut wedges and inoculated 2 jars of rye each.
From BRF pucks I inoculated with agar on 1-6, I scraped some of the growing mycelium up and dropped it into a jar. Each puck got their own jar.





So today:
Plate 026 -> Transferred to 1x BRF plate, 1x Charcoal plate, and Inoculated 2x Rye Grain
Plate 028 -> Transferred to 1x BRF plate, 1x Charcoal plate, and Inoculated 2x Rye Grain
Puck 042 -> Inoculated 1x Rye Grain Jar
Puck 043 -> Inoculated 1x Rye Grain Jar


Today's update, I tried to make sure I got some of the agar in the grain close to the glass to be able to document it as it grows.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Let's All Grow Mushrooms 2025 [Re: Axman] * 1
    #29086284 -

any problem starting new strains as things progress, or better start and finish with what we started with as much as possible?


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Learn it, know it, live it


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Re: Let's All Grow Mushrooms 2025 [Re: Mackload] * 5
    #29086329 -

Mackload said:
any problem starting new strains as things progress, or better start and finish with what we started with as much as possible?


Well the goal is to get from spores to fruits any time after 1-1-2025 so you're free to do whatever varieties or species you want.

If you want to use it for LAGM just make sure it's spores to agar to fruits.

For posterity and the sake of seeing things through, please try your best to run the first ones you start with to completion, no harm in adding additionals IMHO.

I'm getting a gourmet print in the mail in the next few days that I plan on documenting here as well.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Let's All Grow Mushrooms 2025 [Re: Axman] * 4
    #29086563 -

Big transfer day.

3rd Eye (from clones) … did 7 total, mostly T2, 2 were T3 … still on the scenic route to take these back to spores. The tub reboot gave me two tiny fruits and three aborts, seemed liked better off going back to a full tub to eventually get a better shot at swabs.

TW x APE (from LC) …. Got 5 transfers to T1 … will start narrowing down after T2 then try to fruit for spores (and trip…)

SNAPE (from LC) … got 2 T1 …. Same game plan. Have one more T0 to transfer from but needs a few more days.

Have 3 Fiji from spores in BRF puck and 3 Hillbilly from LC in the same … on D2, still no growth





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--------------------------------------------------------------------


Wake up to find out...(~);}

LAGM 25 GLOG


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Re: Let's All Grow Mushrooms 2025 [Re: wichita_jack] * 5
    #29086646 -

1/12 Shiitake spores on agar.



--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Let's All Grow Mushrooms 2025 [Re: RoscoeReturns] * 2
    #29087034 -

RoscoeReturns said:
1/12 Shiitake spores on agar.




Shiitakes are gorgeous :heart::heart:

:nicesmile:

ps,
Irrelevant to the thread but i have a special spot in my heart for them



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:ancientaliens:

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Re: Let's All Grow Mushrooms 2025 [Re: astra] * 6
    #29087044 -

Yesterday I made the worst transfer ever. Hadn't been at home for a couple of days and it was about time for a T1... So got home thoroughly baked and fumbled around in the SAB. I stabbed the plate like 10 times because I didn't hear the hiss on the first pass. Then crudely cut out some really heavy icosahedron and smacked it into a new plate. No pride. If this doesn't contam it's pure luck.


--------------------
Also I tooted churp for being a lowbrow puppet, it's so obvious.
I also added them both to my nazi watchlist in my journal, so we can watch them

-Jean-Luc Picard

Species grown so far (yes, it's a link)

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Re: Let's All Grow Mushrooms 2025 [Re: Churpee Choozle] * 3
    #29087068 -

The beauty of agar! Contam or not, it’ll be fun to watch it grow and find out :smile:


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Re: Let's All Grow Mushrooms 2025 [Re: DV8DUG] * 4
    #29087167 -

DV8DUG said:
1-13-2025

Pan Cyanescens

My first time growing with anything outside of P.Cubensis. It may seem like I went a little overboard with the germ plates but I was testing to see if different nute values affected germination. As you can see plates are color coded by % value of nutes:
  • Red = 4.0%
                  10g agar powder
                  20g LME
                  500ml water
                  10 drops red food coloring
  • Blue = 1.6%
                  10g agar powder
                  8g LME
                  500ml water
                  10 drops blue food coloring
  • Black = 1.0%
                    10g agar powder
                    5g LME
                    500ml water
                    10 drops black food coloring







Here is a sample of a few plates that were looking good to me.



Now I have no experience with this mycelium type. What I have gathered from the site here is I'm looking for fast and whispy.
What does everyone think about any of these plates?





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Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation

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Re: Let's All Grow Mushrooms 2025 [Re: DV8DUG] * 7
    #29087583 -

Germination!!! OkraNat top / x7x+ bottom:



About to transfer them, check out my new SAB bright light:


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Let's All Grow Mushrooms 2025 [Re: KlarnEvil9] * 4
    #29087749 -

KlarnEvil9 said:
Germination!!! OkraNat top / x7x+ bottom:



About to transfer them, check out my new SAB bright light:



I bet that light is awesome for transfers.

How do you like the holy grail plates?

LAGM tax:


The agar and rye seem to be introducing themselves.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Let's All Grow Mushrooms 2025 [Re: Axman] * 2
    #29087781 -

I really dig the HGs all around. My only minor complaint is that sometimes the condensation leaves behind water spots on the lid, making it harder to see through. But that happens rarely, so I'm definitely team HG.


--------------------
"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: Let's All Grow Mushrooms 2025 [Re: KlarnEvil9] * 9
    #29087786 -

First cubes of 2025



--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Let's All Grow Mushrooms 2025 [Re: Axman] * 4
    #29087977 -

Axman said:
KlarnEvil9 said:
Germination!!! OkraNat top / x7x+ bottom:



About to transfer them, check out my new SAB bright light:



I bet that light is awesome for transfers.

How do you like the holy grail plates?

LAGM tax:


The agar and rye seem to be introducing themselves.


I must be really damn old and off the grid, didn't know they call screw on plates 'Holy Grail'.... wow :oldman:

Been using them forever, probably have around 150ish in stock, they make excellent print containers for some reason :heart::heart:



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:ancientaliens:

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Re: Let's All Grow Mushrooms 2025 [Re: astra] * 5
    #29088106 -






That moment when all new germinations look like pan myc 🤣 wth

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Re: Let's All Grow Mushrooms 2025 [Re: HoodedBingling] * 1
    #29088246 -

Oof, be careful, those are pretty contaminated.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Let's All Grow Mushrooms 2025 [Re: Mr Piggy]
    #29088326 -

Bacterial colonies from the swab streaking. Not in the myc itself. Just transfer away right?

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Re: Let's All Grow Mushrooms 2025 [Re: HoodedBingling] * 1
    #29088338 -

HoodedBingling said:
Bacterial colonies from the swab streaking. Not in the myc itself. Just transfer away right?


:asianofapproval:
Looks like maybe the mystery SA pan plate was a mispour,  so maybe not as bad as they look initially.  Should be fine transferring away from that if you're quick.


--------------------

THE 49TH OYSTER BUCKET TEK

FREE WILL IS A SHADOW

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Re: Let's All Grow Mushrooms 2025 [Re: alaskappalachian] * 2
    #29088350 -

Yeah the irregularity is in the agar. The myc itself looks fine to me in person. Only visible contam is the bacteria in some of the swab plates.
I also got a few moldy ones but those r gone

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Re: Let's All Grow Mushrooms 2025 [Re: HoodedBingling] * 3
    #29088437 -

already transferred this rhizo but kinda crazy how distinct it now is... prob gonna get another T2 so im 100% sure i got it!



:feelsgoodman:


--------------------
:mushroom2: panta rhei :mushroom2:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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