MycCycle said:

ThurmanMurman said:

Mr Piggy said:

Once you get bored of collecting perfect cultures, you can do things like pursue slow growing low yield pretty FREAKS.

---

I just made some PE LC, and I'm putting the natalensis away for now. It's funny how much of a hurry we are at the starting point and then later think "holy shit man, this has kinda turned into a lot and I want a break."

---

"Hmm, why not put this and this on agar as well, and maybe this while I wait for the first ones?"

Just to find you ran through more plates in a week month than you would ever believe you could need in a year...

---

Smellyhobbit said:

I feel so seen

---

ThurmanMurman said:

I deny these allegations and have no further comment.

---

MycCycle said:

Smellyhobbit said:

I feel so seen

---

Your signature certainly is.
Those are so white!

Closest I have, I think, is Storm Trooper. However, I cannot for the life of me get it clean...
What is it?

---

It’s an APE cross I’m working on stabilizing called Kodama

Phaethon said:

For actually keeping track of them, the world is your oyster (mushroom). The freezer boxes for those cryovials have nice Battleship-style row/column labels (A-J, 1-10), so you can write down the "coordinates" for each culture so you don't have to take each out individually to find something. I've also just written on the box lid (Sharpie is erasable with alcohol). You can use a spreadsheet, a text file, or a plain old paper notebook. There's probably off the shelf software that would also help with organizing.

---

PrinceOfCats said:

What kind of contamination I m looking at?

---

Blueberry Muffin said:

Is spore swab to brf puck an actual legit thing to do? I would think agar is your best chance at actually transferring away from contamination

---

My plan was to transfer away a plin to agar, but the Nats I did seemed to stall out. I think my mixture was on the dry side.

The main reason I'm doing swab to brf pucks is because the last time I did a swab to agar I ended up with plates that were more bacteria than mycelium. I spent months trying to clean them up, but couldn't get a clean culture even with water agar, josex poke, etc...

FWIW, it seems to be working. Here are some pics from some glass petris that I transferred brf material to:







They will still need a couple transfers to clean up, but as a starting point they seem to be in much better shape.

Edit: I'm thinking of taking the following transfers but any opinions are welcome:

Pic 1: 9-11 o clock
Pic 2: toss
Pic 3: 6-9 o clock
Pic 4: 3-4 7-10 11-12

Edit 2: I just realized Pic 2 is agar to agar not brf to agar. I must've mislabeled it. Still going to toss it.

Blueberry Muffin said:

Is spore swab to brf puck an actual legit thing to do? I would think agar is your best chance at actually transferring away from contamination

---