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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 3
    #29013903 -

callmehippie said:
So I know it's not necessary.

but I'm curious..

When inoculating a puck or plate with a swab

Who dips the swab into water first?

Who dosnt dip the swab into  water first?

Whats the difference?



If there are enough spores on the swab to the naked eye you got more than enough to streak it dry. I have never needed to or ever add water to the equation.

Never swabbed to a puck before. Just plates

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29013949 -

Huskies said:
...perhaps my lids are not enough. When I use the standard ring ones, I flip them over before I make the whole. SOme of them have been used a lot, I straighten them out if i notice they are bumpy.

Now, if its bacteria during my grain incolucation, would Lysol help sterilize inside the SAB? Spray, cover the holes, wait 15 mins, and then after alcoholign the crap out of my hands and arms, do the transfers?


Nope.  Lysol has no place in this hobby.  You don't need to chemically strip your arms either, soap and warm water are just fine.  There are no shortcuts to having a steady hand and making sure your technique is correct.  No moving hands or unsterilized tools over plates, lids, anything. 

What kind of filters do you have on your lids?


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
    #29013981 -

Has anyone tried these reusable petri dishes? Im trying to get away from single use condiment cups. I thought about getting glass ones but I feel like these would last me longer. How would I go about sterilizing them in the PC? I know 30 mins at 15 psi, but what kind of containers do I pc them in? would I stack them in jars? Ideally id like to sterilize a large batch at once. What is your favorite type of petri dish and why?

Sorry for the stupid long link
https://www.amazon.com/Autoclavable-Vabiooth-Experiment-Non-Fragile-Scratch-Resistant/dp/B0C954KGQW/ref=sr_1_3?crid=CU73KRUPJROV&dib=eyJ2IjoiMSJ9.KInJB5PHhhQvOp460FB3o85JxymHZXv42F-IrDBYb6a2nFDi7w9us6emD1KqcrdL3sdDj7M8yOr3H0C1TX67PfllSVYUy6DZO3llCVxQVaAroWBvcIL45IvCuEov9xdkhx-Twpx52o1uTSH6VlkInWMjXnAKjklIfSff5veY5fDZ7fZErWNzW-3Ui-kjyBmPLtAfh3FtQTnzv-4IcIfZwWP94yVRB86UrWuJjQk7bT0.Pr1m_eEb18O82oma9DywRR2rPjKY5i6xhF52fJI4SnA&dib_tag=se&keywords=reusable%2Bpetri%2Bdishes%2Bwith%2Blids%2Bpp5&qid=1730862379&sprefix=petri%2Bdishes%2Bre%2Caps%2C220&sr=8-3&th=1

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Re: Ask Quick Questions, Get Quick Answers [Re: Amethyst_Scepter]
    #29014002 -

I love the 60mm version of those. You can put them in wide mouth jars (8 in a pint, 10 in a quart or maybe 11 if you squeeze). I like to use a strip of parchment as a sling to put them in and take them out of the jars. PC at the same time as your agar.
I put them back in the jars, unwrapped, after pouring, to store until I use them.


I'm sure the 90mm ones are great too. Not sure the best vessel for autoclaving them, but I'd guess you could get away with wrapping in foil or using a filtered grow bag. I think ReverendMyc has a post about those (he likes to pre-pour them; I could never get the condensation level low enough and I really don't mind pouring plates).


--------------------
LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Radamanto]
    #29014095 -

Radamanto said:
What you described is what was happening to Josex on the thread I linked. His spawn was 100% clean it just looked off because of the grain quality or how it was stored beforehand or some other variable. But it was performing decently when spawned to tubs


Alright, so some bacteria has that Call of Duty perk where ya drop a grenade on death? Thats fuckin cool - as if we didnt have enough problems...🤣🤣

I had a bad feeling when I got to the part where he inoculated with LC and then transferred back to agar from the jar and had no growth. Excuse me, what? Live myc went in and dead myc came out? Fuckin how? Oh toxic bacteria ass juice is how? Groovy...


Im not about to assume this is my problem bc I gotta make sure I dont just suck before getting into all that, but that was a good read, and kinda freaky, tbh.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29014469 -

Pin mold, I know, my question: is everything pin mold mycelium? Plates are 2 days.




--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29014471 -

Seems that way, you're isolating the pin mold.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #29014476 -

Thanks, I had no intention of trying to clean up, but as a noob I need to know what I am looking at, and what to be looking for going forward.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Amethyst_Scepter]
    #29014699 -

Amethyst_Scepter said:
Has anyone tried these reusable petri dishes? Im trying to get away from single use condiment cups. I thought about getting glass ones but I feel like these would last me longer. How would I go about sterilizing them in the PC? I know 30 mins at 15 psi, but what kind of containers do I pc them in? would I stack them in jars? Ideally id like to sterilize a large batch at once. What is your favorite type of petri dish and why?

Sorry for the stupid long link
https://www.amazon.com/Autoclavable-Vabiooth-Experiment-Non-Fragile-Scratch-Resistant/dp/B0C954KGQW/ref=sr_1_3?crid=CU73KRUPJROV&dib=eyJ2IjoiMSJ9.KInJB5PHhhQvOp460FB3o85JxymHZXv42F-IrDBYb6a2nFDi7w9us6emD1KqcrdL3sdDj7M8yOr3H0C1TX67PfllSVYUy6DZO3llCVxQVaAroWBvcIL45IvCuEov9xdkhx-Twpx52o1uTSH6VlkInWMjXnAKjklIfSff5veY5fDZ7fZErWNzW-3Ui-kjyBmPLtAfh3FtQTnzv-4IcIfZwWP94yVRB86UrWuJjQk7bT0.Pr1m_eEb18O82oma9DywRR2rPjKY5i6xhF52fJI4SnA&dib_tag=se&keywords=reusable%2Bpetri%2Bdishes%2Bwith%2Blids%2Bpp5&qid=1730862379&sprefix=petri%2Bdishes%2Bre%2Caps%2C220&sr=8-3&th=1


Here is a tek on them specifically. There is a link to jars that works for the 90 mm in the comments.
They get a lot of condensation when you do pre-pour plates, but there are ways of dealing with that. And you can also sterilize them empty and then do standard pours which helps with condensation, marginally.

P.S. You can remove everything after the item number in an Amazon link and still get the same result.
https://www.amazon.com/Autoclavable-Vabiooth-Experiment-Non-Fragile-Scratch-Resistant/dp/B0C954KGQW/
https://www.amazon.com/gp/product/B0CMWN25LR


--------------------
Stoned Gummys | BRF Pucks | Primo Reusable Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic

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Re: Ask Quick Questions, Get Quick Answers [Re: ReverendMyc]
    #29014875 -

Ive got some PE spawn almost ready. Sub prep is the same as any other cube but just with a thicker top layer of coir, yeah? I searched but then saw people discussing pretty low spawn ratios, so I just wanted to verify that wasn't considered necessary. Figured Id do a 6qt with 1:2+2qt top layer and call it a day?


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: Amethyst_Scepter]
    #29014891 -

Amethyst_Scepter said:
Sorry for the stupid long link


Check this out - It's easy to remove the extra tracking garbage from links!

Edited by KlarnEvil9 (11/06/24 12:24 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman] * 1
    #29014902 -

ThurmanMurman said:
Ive got some PE spawn almost ready. Sub prep is the same as any other cube but just with a thicker top layer of coir, yeah? I searched but then saw people discussing pretty low spawn ratios, so I just wanted to verify that wasn't considered necessary. Figured Id do a 6qt with 1:2+2qt top layer and call it a day?


I’ve grown a few PE and have some in bulk right now.  I use 1:1.5 with pseudo casing, let colonize then add about an inch final casing.  Didn’t blob at all, but took a lot longer to fruit.  Did not have to mist until 2nd flush


--------------------
Beef’s The Hitchikers Guide to Shroomery
Stipe’s Clean Spawn Checklist
Gummies by milkboy
Eat’s Easy Oats Prep
DV8DUG’s Recognizing Contamination in Grain Jars

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Re: Ask Quick Questions, Get Quick Answers [Re: Headass]
    #29014914 -

Enigma plate "pin" clone. Should I transfer or is this good to put to grain?



--------------------



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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #29014922 -

Should be fine to put directly to grain if that piece of tissue came from a clean plate.


--------------------
LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Headass]
    #29014937 -

Headass said:
ThurmanMurman said:
Ive got some PE spawn almost ready. Sub prep is the same as any other cube but just with a thicker top layer of coir, yeah? I searched but then saw people discussing pretty low spawn ratios, so I just wanted to verify that wasn't considered necessary. Figured Id do a 6qt with 1:2+2qt top layer and call it a day?


I’ve grown a few PE and have some in bulk right now.  I use 1:1.5 with pseudo casing, let colonize then add about an inch final casing.  Didn’t blob at all, but took a lot longer to fruit.  Did not have to mist until 2nd flush


I pseudo case everything anyway, but may I ask why your ratio is 1:1.5? Is that including the top layer or is it closer to 1:3, including the top layer. I understand they're slow to fruit, but colonization speed is typical of cubes, yes?


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29014948 -

A few (truly) QQ:

1. Do you veteran growers save an MS plate after grabbing your choice sector and then return to it if you dont care for the results? Trying to reduce # of plates sitting around.

2. Do you guys see plates, coir and tubs go on sale for black friday?

3. Are veteran growers still wiping down plates and jars with iso before working in the flow or is 5 min or so of pre work breeze time sufficient? Im trying to get away from the obsessive wiping.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29014977 -

ThurmanMurman said:
Headass said:
ThurmanMurman said:
Ive got some PE spawn almost ready. Sub prep is the same as any other cube but just with a thicker top layer of coir, yeah? I searched but then saw people discussing pretty low spawn ratios, so I just wanted to verify that wasn't considered necessary. Figured Id do a 6qt with 1:2+2qt top layer and call it a day?


I’ve grown a few PE and have some in bulk right now.  I use 1:1.5 with pseudo casing, let colonize then add about an inch final casing.  Didn’t blob at all, but took a lot longer to fruit.  Did not have to mist until 2nd flush


I pseudo case everything anyway, but may I ask why your ratio is 1:1.5? Is that including the top layer or is it closer to 1:3, including the top layer. I understand they're slow to fruit, but colonization speed is typical of cubes, yes?


They are slow to fruit yes.  I don’t have rhyme or reason for ratio except it works for my area.  I wouldn’t suggest that this would the only way.  I guess an additional reason was that I wanted to avoid blobbing.  The current run I have I decided to only pseudo casing and not a top case to see what, if anything, changes.

Forgot to add, I went to bulk on Oct 19 with multiple shoeboxes of PE, GT, meanies and B+, all with the same pseudo casing… the non-PE started pins about two days ago, the PE started this morning, so a top casing, as expected, extends to time to fruit.  Just an initial observation of mine


--------------------
Beef’s The Hitchikers Guide to Shroomery
Stipe’s Clean Spawn Checklist
Gummies by milkboy
Eat’s Easy Oats Prep
DV8DUG’s Recognizing Contamination in Grain Jars

Edited by Headass (11/06/24 02:21 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Headass]
    #29014991 -

What's the best/easiest fruiting chamber thread you can think of

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Re: Ask Quick Questions, Get Quick Answers [Re: Xclozure] * 1
    #29014995 -

Xclozure said:
What's the best/easiest fruiting chamber thread you can think of


For cubes? Pasty’s ez dial


--------------------
Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

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Re: Ask Quick Questions, Get Quick Answers [Re: EniQma]
    #29015007 -

Ty Eniqma ! any other ones I should see that's more recent? Someone just told me most people don't do holes in their tubs or use verm anymore?

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Shop: North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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