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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Arkyhog]
    #28945974 -

Yessir I’ll post one shortly

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Re: Ask Quick Questions, Get Quick Answers [Re: PsillySeeEms]
    #28945985 -

PsillySeeEms said:
Yes, IP will sterilize agar properly. I do 45 minutes to be safe, and haven't had a problem making them up, even with no-pour containers that only have a thin 15ml layer on the bottom. You would think no pours being spread out would crystalize faster than a flask full of the agar solution. Nope.


Thanks for the info, I'll cook it longer then!

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Re: Ask Quick Questions, Get Quick Answers [Re: Romes]
    #28946151 -

I think these jars look good? It's been a long time since I've grown... like back when I used to just inject ms syring into grain. This was done with spores to agar and then 4 transfers.

There's some water on the bottom, I assume it's exudate and I shouldn't worry about it?

Thanks!




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Re: Ask Quick Questions, Get Quick Answers [Re: StalkOfFomentarius] * 3
    #28946154 -

Yarr that be bacterial 🏴‍☠️


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28946159 -

I think I stuffed the polyfil too tight and there's not enough fae :frown:

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Re: Ask Quick Questions, Get Quick Answers [Re: StalkOfFomentarius] * 3
    #28946160 -

That and it’s bacterial


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 2
    #28946161 -

:whathesaid:

Very Bacterial, in fact...

Edited by RockinRobot (09/14/24 02:12 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: StalkOfFomentarius]
    #28946162 -

StalkOfFomentarius said:
I think I stuffed the polyfil too tight and there's not enough fae :frown:


How did you prep the grains? I think I see mold in jar a2 as well. Grain prep is a critical step.


--------------------
🌾💮🫘🥖

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Re: Ask Quick Questions, Get Quick Answers [Re: hellofresh]
    #28946223 -

:whathesaid:  if not the most critical...

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #28946262 -

there's always mold and bacteria after you hydrate the grain, correctly or incorrectly, but all life forms shall be killed in proper sterilisation process.
It's really difficult to "stuff the polyfil too tight" and, if you eventually do, what you will notice is stale growth.
...and yes, mycelium and bacteria, in that jar, had enough oxygen to break down nutrients and convert them into energy that is needed for growth.


--------------------
“All this buttoning and unbuttoning”

Edited by 999999999 (09/14/24 04:43 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: 999999999] * 1
    #28946300 -

Do these jars look ready for coir?

Edited by Dad_Shroomer (09/14/24 05:35 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet]
    #28946354 -

I cloned a tiny p mexicana that fruited on agar. It was to small to split and take tissue from the center of the stem so I cut the mushroom into 4 pieces and put each on its own agar dish. All 4 are showing growth. Will the cap portion potentially contain multiple genetics? Or is that risk minimized as it was an immature mushroom?

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Re: Ask Quick Questions, Get Quick Answers [Re: Amethyst_Scepter]
    #28946356 -

I don’t know about your question, but you don’t need to take interior tissue with a plate pin. Just move the whole thing. Cutting it up is fine too for multiple plates, but sterility is insured by it fruiting in vitro.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28946362 -

Thank you, that was what I was thinking as well but its my first plate pins!

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Re: Ask Quick Questions, Get Quick Answers [Re: Amethyst_Scepter]
    #28946409 -

How concrete of a conclusion is it to determine a spawn jar is clean by shaking and observing complete recovery multiple times over? Ive got a jar I thought might be sketchy, but I've shaken and watched it fully colonize again twice over, so it has fully colonized a total of 3 times and I see no signs of contam. I initially wanted to use it for g2g, so was hoping this would be enough to be confident it's clean.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: hellofresh]
    #28946429 -

soaked oats over night.
15 psi in the pressure cooker for 90 minutes.
Let the pressure cooker cool down naturally.
I actually leave the jars in the cooker until it's time to move them into the still air box.
My plates looked good.

I wonder if my pressure cooker could be the issue. My gauge never goes over 12psi but the rocker definitely starts to move.. meaning it should be at 15psi even if the gauge is off... right?

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Re: Ask Quick Questions, Get Quick Answers [Re: StalkOfFomentarius] * 1
    #28946430 -

Depends on the rocker. Go 120 minutes going forward just to be sure


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28946433 -

Gauge is probably off.  Many are.  x2 on the above.  I often run mine 120 on GP since it doesn't require additional effort.

@Thurman it's a pretty damn good indicator.  You could observe a late stage competitor mold, but that's what the consolidation period really accomplishes IMHO (screening out the sneaky late shit snafus).  Definitely not bacterial if it recovers multiple times. 


--------------------

THE 49TH OYSTER BUCKET TEK

FREE WILL IS A SHADOW

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Re: Ask Quick Questions, Get Quick Answers [Re: alaskappalachian]
    #28946449 -

Is it obvious when LC is contaminated? Ive never messed with LC before, but I recently poured some in a few freshly sterilized grain jars. It looked super clear and clean and is colonizing the grains well. Im assuming if there was a problem with the LC it would be evident at this point?

I know the official recommendation is to test it but to me it just seems the very act of testing it would open it up to contamination, thus making the test pointless. I got my LC in an agar media bottle


--------------------



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Re: Ask Quick Questions, Get Quick Answers [Re: AspectOfTheCreator]
    #28946458 -

AspectOfTheCreator said:
Is it obvious when LC is contaminated? Ive never messed with LC before, but I recently poured some in a few freshly sterilized grain jars. It looked super clear and clean and is colonizing the grains well. Im assuming if there was a problem with the LC it would be evident at this point?

I know the official recommendation is to test it but to me it just seems the very act of testing it would open it up to contamination, thus making the test pointless. I got my LC in an agar media bottle


exactly my thoughts :lol:
But it's not obvious at all when the myc has company...
I just feel serene with josex poke and a quick squirt at it, but after that I feel like I'm going all-in without seeing my cards
:prayer:


--------------------
“All this buttoning and unbuttoning”

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Shop: Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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