GenesisCorrupted said:

That actually looks normal to me because he inoculated with LC. It inoculates all the way down where it flowed through the jar and it usually starts a ring at the bottom.

Crazy textures in that jar though.

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callmehippie said:

How resilient is mycelium? If you've got super clean healthy spawn. Could you essentially break and shake it as many times as you want and it'll just keep recovering?

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ansr said:

Whats the reason for a single or a few single wheat grains refusing to get colonized?
I don't think they are contaminated. Picture from a week ago but one or two grains in the jars are still not colonized.
Should I wait or can I just pick them out?

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Phaethon said:

You don't need to test anything on agar to use PF tek. The whole point is its resistance to bacterial contamination and small size so in the event of a rogue contaminant, you've only lost a little jar.

You can also go from BRF to agar, once you have germination. Just make a short BRF puck without a top layer of dry verm, and inject it with the syringe in a still air box. When it's got nice growth you can cut out with a scalpel, make that transfer to agar.

The point of LC is to inoculate grains or sometimes Muda bottles (all in ones, look up Muda bottle tek). It usually goes faster than agar wedges, so people like it for that, and it's easy to make enough to inoculate a ton of jars with very little effort. Requires care to ensure it's squeaky clean.

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callmehippie said:

Phaethon said:

You don't need to test anything on agar to use PF tek. The whole point is its resistance to bacterial contamination and small size so in the event of a rogue contaminant, you've only lost a little jar.

You can also go from BRF to agar, once you have germination. Just make a short BRF puck without a top layer of dry verm, and inject it with the syringe in a still air box. When it's got nice growth you can cut out with a scalpel, make that transfer to agar.

The point of LC is to inoculate grains or sometimes Muda bottles (all in ones, look up Muda bottle tek). It usually goes faster than agar wedges, so people like it for that, and it's easy to make enough to inoculate a ton of jars with very little effort. Requires care to ensure it's squeaky clean.

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Do you have to take any extra measures when making the brf puck without the top dry layer of verm? Cuz doesn't that dry layer help keep it from getting contaminated from the top? Should I be putting micro four tape or something over the holes if I'm not doing the dry layer?

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