Kinoko314 said:

Fiet said:

The size good for LC??


Edit: Guy's need a LC Jar lid a Air Exchange Hole ore is a Syringe Filter enough?

Thx

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Any size is good for LC.  It just depends how much you want to make.  I like to only fill mine half way or less, so I usually use larger jars on the rare occasion I do LC.  A syringe filter is sufficient for LC.

ThurmanMurman said:

Guys, I cant figure out what I'm doing wrong here, but its always this little gray POS...if this was riding on the plate that I used, then I might as well just quit, bc that plate looked pristine to my eyes. Work done in verified laminar flow, never hovered above my work...I just told my gf the other day that if these jars contaminated, I was in trouble, bc I cant find anymore holes to plug in my technique. The quart jars have unmodded lids and I must've fucked the dog and not loosened enough during sterilization, bc theyre a bit warped and difficult to turn. Any chance my lids are at fault? Im 99% sure I can discard my copium idea of that being weak myc, though would be funny if it was bc I've tossed a lot of shit with that slightly grey shit on it. I've Never seen pics of that being passed off as myc though, so I won't kid myself.

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It's unlikely that it was your PC cycle, since mold spores die pretty quick in the heat.  It's probably getting in when you inoculate.  It could be an issue with your lids.  I don't have much experience with unmodified lids, but if things are warped and the lid isn't getting close to a full seal all around, it could lead to contamination.  Even having it unscrewed a little too much could potentially be a problem.

One thing I wanted to point out is that you talked about not hovering over your work.  That's perfect for SAB work, but in front of a flow hood you need to be thinking about "first air."  You don't want anything getting in between the flow and your work.  It's basically the same thing turned 90 degrees.  I don't know if that's your issue, but it's something to keep in mind.

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Quote:

Azhiaaziam said:Did that compromise the sterilization?

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notthatsorry said:

Quote:

Azhiaaziam said:Did that compromise the sterilization?

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Not in my experience. Every PC cycle I've ever run has ended up smelling like whatever was in the PC.

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Azhiaaziam said:

I did my first batch of Agar using Bods Tek, same bottle and everything.

When I opened my PC I noticed I could smell malt, and the water used to steam had become cloudy.

This seems to indicate that some of the agar solution came out of the bottle and into the water.

Does this mean my lid was too loose? Did that compromise the sterilization?

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Azhiaaziam said:

I did my first batch of Agar using Bods Tek, same bottle and everything.

When I opened my PC I noticed I could smell malt, and the water used to steam had become cloudy.

This seems to indicate that some of the agar solution came out of the bottle and into the water.

Does this mean my lid was too loose? Did that compromise the sterilization?

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Quote:

SPECIES_% PSILOCYBIN_% PSILOCIN_% BAEOCYSTIN_REFERENCE
P. azurenscens ______1.78_____ .38_____.35_________ Stamets and Gartz 1995
P. bohemica _________1.34______.11_____.02_________ Gartz and Muller 1989; Gartz (1994)
P. semilanceata______.98_______.02_____.36__________Gartz 1994
P. baeocystis________.85_______.59_____.10__________Repke et al. 1977; Beug and Bigwood 1982(b)
P. cyanescens________.85_______.36_____.03__________Stijve and Kuyper 1985; Repke et al. 1977
P. tampanensis_______.68_______.32______n/a_________Gartz 1994
P. cubensis__________.63_______.60_____.025_________Gartz 1994; Stijve and de Meijer 1993
P. weilii____________.61_______.27_____.05
P. hoogshagenii______.60_______.10_____n/a___________Heim and Hofmann 1958
P. stuntzii__________.36_______.12_____.02___________Beug and Bigwood 1982(b); Repke et al. 1977
P. cyanofibrillosa___.21_______.04_____n/a___________Stamets et al. 1980
P. liniformans_______.16_______n/d_____.005__________Stijve and Kuyper

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DERRAYLD said:

Bigdogg said:

The amount of condensation is a little concerning. It is a little foamy on top and although fully colonized there are a couple bald spots.

I can't tell if there are grey/green spots or if it's bruising or reflections.

I would send it. I'm sure you'll get at least a first flush. The myc might even wipe out any contam once it hits bulk sub and air.

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Cubensis mycelium won't wipe shit out, that's just false info.

Getting a flush is not related to the mycelium winning some mythological fight against evil.

Contaminated is contaminated.

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callmehippie said:

Bigdogg said:

The amount of condensation is a little concerning. It is a little foamy on top and although fully colonized there are a couple bald spots.

I can't tell if there are grey/green spots or if it's bruising or reflections.

I would send it. I'm sure you'll get at least a first flush. The myc might even wipe out any contam once it hits bulk sub and air.

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Cool thanks for the advice. I was worried about the condensation as well. I've got a mini inflatable monotub (about 44qt) and I've got a bunch of shoe boxes.  I'm sure it's probably personal preference. But I could have higher chance of success rate if I break it up into shoe boxes as opposed to Throwing It All in One tub right?

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