ThurmanMurman said:

RoscoeReturns said:

ThurmanMurman said:

So I'm working on my ffu sterile technique, after a botched first attempt at A2G. I understand not to put my hands over open vessels and dont put anything between filter and my work. So one issue I could imagine causing problems was with the steps involving cutting up the plate. I work on a cookie rack, started out opening HG style plate, laid the lid on cookie rack with the top down, did my cutting, and then set the cup back on the lid upside down. Unscrewed grain jar lid almost all the way, flamed scalpel again, cooled it in the flow for maybe 5 seconds, then removed lid, set it top side down on the rack, used my scalpel to flick agar into the jar, then put the lid back on the jar. So my question is whether or not I should be placing the plate face down on the lid, bc it's pretty much impossible to do perfectly without touching the rim of the cup to the rim of the lid, and the rim of the lid was exposed to open air the entire time it was colonizing, so I'm wondering if that's where I picked up nasties. Disclaimer- this isn't something I considered until after the fact, and not something I did while actively wondering if it was a problem. Is this considered procedurally accurate or should I just leave the cup face up in the flow while I finish prepping the jar and scalpel?

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I never put down lids on anything. Lift lid off plate, take wedge, put lid on plate. Take lid off jar and drop wedge in. Put lid back on jar. I never put down a lid. I never put down my scalpel. Unscrew jar lids and unwrap plates before I flame my blade so I can do everything with one hand.

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You're only putting a single wedge in a quart of grain? I thought the procedure was to slice the whole plate into a grid and flick them into the jar. I suppose I could unscrew the lid entirely so it's just sitting on the jar, slice up the plate, remove lid and transfer and then replace lid. Not even dick around with setting down the plate or flaming again...

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StophJS said:

Not really a question, but I asked a lot of questions recently about why my grainspawn keeps failing. To update: I did not yet change my lids or jars, but I did switch to reverse osmosis water, PC for two hours and inoculate in SAB by squirting fresh tested LC syringe directly onto grains instead of through SHIP. Still get this growth. For just a moment I thought there was healthy growth, but it only looked normal between the glass and first grains. Between grains it turns into this. No bacterial smell or otherwise obvious wet spot or sourness.

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longstrangetrip8 said:

Hey everyone

I noticed in an ezdial tub, one side  has far less condensation  near holes than the other sides

Worth putting micropore tape on that side (and not the others), or would that ruin flow of FAE....  or is this not even worth worrying about?

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RoscoeReturns said:

ThurmanMurman said:

RoscoeReturns said:

ThurmanMurman said:

So I'm working on my ffu sterile technique, after a botched first attempt at A2G. I understand not to put my hands over open vessels and dont put anything between filter and my work. So one issue I could imagine causing problems was with the steps involving cutting up the plate. I work on a cookie rack, started out opening HG style plate, laid the lid on cookie rack with the top down, did my cutting, and then set the cup back on the lid upside down. Unscrewed grain jar lid almost all the way, flamed scalpel again, cooled it in the flow for maybe 5 seconds, then removed lid, set it top side down on the rack, used my scalpel to flick agar into the jar, then put the lid back on the jar. So my question is whether or not I should be placing the plate face down on the lid, bc it's pretty much impossible to do perfectly without touching the rim of the cup to the rim of the lid, and the rim of the lid was exposed to open air the entire time it was colonizing, so I'm wondering if that's where I picked up nasties. Disclaimer- this isn't something I considered until after the fact, and not something I did while actively wondering if it was a problem. Is this considered procedurally accurate or should I just leave the cup face up in the flow while I finish prepping the jar and scalpel?

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I never put down lids on anything. Lift lid off plate, take wedge, put lid on plate. Take lid off jar and drop wedge in. Put lid back on jar. I never put down a lid. I never put down my scalpel. Unscrew jar lids and unwrap plates before I flame my blade so I can do everything with one hand.

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You're only putting a single wedge in a quart of grain? I thought the procedure was to slice the whole plate into a grid and flick them into the jar. I suppose I could unscrew the lid entirely so it's just sitting on the jar, slice up the plate, remove lid and transfer and then replace lid. Not even dick around with setting down the plate or flaming again...

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You can put multiple wedges in a jar. I do sometimes. You can also just do a single wedge. The result is the same it may just take slightly longer. If you’re not having pretty consistent success I would just use one wedge. Make your transfers quick and easy, and limit the chance of contamination.

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flyboi said:

What is the most logical pasteurization technique in 2024? Bucket is not the only reliable one, I use verm/gypsum/coir.

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Quote:

maybe not so quick question please fellas

When pasteurizing, are people ramping up slowly and ramping down slowly to/from temperature? What is the best practice?

I have been wetting the sub in the vaccuum bags with 80°C water to get close to 69°C from the start, then sous vide for 2 hours once bath is at temperature plus an extra ten or fifteen minutes to allow for temp equalisation of my bags, which are made very thin, so that they come to temperature with the water very rapidly as it is climbing to temp with the sous vide

When I finish I just remove them and leave them on a shelf till cooled, then cut a slit to allow some air back in so everything is not anaerobic in there while they wait a day usually to get spawned

does this all sound alright and like best possible practice?

Or should I be rapidly cooling bags after pasteurization in a cool water bath and/or allowing them to rise to temp over an hour or more as the sous vide warms the bath?

I also assume I should be allowing air back in also and not leaving them sealed and vaccuumed there until use?

My process works fine it seems but I wonder what the gold standard is?

Thanks, good times

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SingularFusion said:

repost of unanswered question from a month ago please

Quote:

maybe not so quick question please fellas

When pasteurizing, are people ramping up slowly and ramping down slowly to/from temperature? What is the best practice?

I have been wetting the sub in the vaccuum bags with 80°C water to get close to 69°C from the start, then sous vide for 2 hours once bath is at temperature plus an extra ten or fifteen minutes to allow for temp equalisation of my bags, which are made very thin, so that they come to temperature with the water very rapidly as it is climbing to temp with the sous vide

When I finish I just remove them and leave them on a shelf till cooled, then cut a slit to allow some air back in so everything is not anaerobic in there while they wait a day usually to get spawned

does this all sound alright and like best possible practice?

Or should I be rapidly cooling bags after pasteurization in a cool water bath and/or allowing them to rise to temp over an hour or more as the sous vide warms the bath?

I also assume I should be allowing air back in also and not leaving them sealed and vaccuumed there until use?

My process works fine it seems but I wonder what the gold standard is?

Thanks, good times

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Tri-Polar said:

callmehippie said:

About how many six quart shoe boxes is it possible to make with 3 lb of Spawn?

I've got a couple 3 lb grain bags I'm interested in spawning out into shoe boxes

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Depends on your spawn ratio, 3-6 comfortably though I reckon






What's a good ratio?

Nichrome said:

Get a sous vide.

You can indeed get away with a nice packed coir casing or topper if you dial in your moisture.

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