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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Psycho D]
    #28888914 -

yes.

Keep them


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Astra said:

“Peace River has a gentle personality, yet TTBVI hit me like strong LSD with a twist of medical grade meth. Not for the faint of heart.”

Team Panaeolus

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Re: Ask Quick Questions, Get Quick Answers [Re: Psycho D] * 1
    #28888916 -

The pins and abourts though small do still contain actives.


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Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: Ask Quick Questions, Get Quick Answers [Re: HILLBILLY OUTLAW] * 1
    #28888921 -

When I harvested. I even kept the stumps. I made stumpy tea. Still very active.


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Re: Ask Quick Questions, Get Quick Answers [Re: TheAtlantean]
    #28888926 -

TheAtlantean said:
yes.

Keep them


Awesome! Thanks for your help


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"Believing in others is to radiate hope and love in life."

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Re: Ask Quick Questions, Get Quick Answers [Re: HILLBILLY OUTLAW]
    #28888927 -

HILLBILLY OUTLAW said:
The pins and abourts though small do still contain actives.


That’s the answer I was hoping for. Thanks!


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"Believing in others is to radiate hope and love in life."

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted] * 1
    #28888930 -

GenesisCorrupted said:
When I harvested. I even kept the stumps. I made stumpy tea. Still very active.


Thanks for the help!


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"Believing in others is to radiate hope and love in life."

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Re: Ask Quick Questions, Get Quick Answers [Re: Psycho D]
    #28889281 -

Grain that doesn't take...

I had a grain bag that didn't take with some LC. The other 2 did. Could I put the bag in a PC for a bit to resterilize and re try inoculating?

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Re: Ask Quick Questions, Get Quick Answers [Re: SkippyCOR]
    #28889313 -

SkippyCOR said:
Grain that doesn't take...

I had a grain bag that didn't take with some LC. The other 2 did. Could I put the bag in a PC for a bit to resterilize and re try inoculating?


Didn’t take? Like the LC didn’t end up colonizing any grain? Did you use enough of the liquid solution? Pictures can help a lot.

Honestly, I would just start over. Maybe reuse the bag? If at all? But if you have a PC, why not just do some jars? Easily reusable and overall great for the environment.


--------------------
Astra said:

“Peace River has a gentle personality, yet TTBVI hit me like strong LSD with a twist of medical grade meth. Not for the faint of heart.”

Team Panaeolus

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Re: Ask Quick Questions, Get Quick Answers [Re: GenesisCorrupted]
    #28889317 -

GenesisCorrupted said:
When I harvested. I even kept the stumps. I made stumpy tea. Still very active.


Stumpy T was my double amputee rapper neighbor in my old hood :lol:


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Learn it, know it, live it


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Re: Ask Quick Questions, Get Quick Answers [Re: TheAtlantean] * 1
    #28889326 -

TheAtlantean said:
SkippyCOR said:
Grain that doesn't take...

I had a grain bag that didn't take with some LC. The other 2 did. Could I put the bag in a PC for a bit to resterilize and re try inoculating?


Didn’t take? Like the LC didn’t end up colonizing any grain? Did you use enough of the liquid solution? Pictures can help a lot.

Honestly, I would just start over. Maybe reuse the bag? If at all? But if you have a PC, why not just do some jars? Easily reusable and overall great for the environment.



I think I put around 3.5cc into it. Its been a few months. There is nothing to show its just a normal grain bag that looks fresh. No contam, no moisture it looks brand new. The mycelium just didn't take I guess.

I have switched to using grain jars and buying grain in bulk, but I have a few grain bags laying around still. I have a bunch of LC so I think I'm going to do an experiment and PC the bag for a bit and re inoculate it. Worst case ill just have to throw it out later.

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Re: Ask Quick Questions, Get Quick Answers [Re: SkippyCOR]
    #28889357 -

All trash or anything good in here? First agar, spore syringe.

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Re: Ask Quick Questions, Get Quick Answers [Re: Letsfly24]
    #28889361 -

Looks like you have bacteria riding along. I would consider taking from the leading edge? And transfer on to a new plate. But could also consider getting a print from the marketplace? Prints are easier to work with IMO.

Just swab and swipe.

If you insist on using the syringe, make sure it’s thoroughly shaken and only apply 1-2 drops. That should ensure a good chance.


--------------------
Astra said:

“Peace River has a gentle personality, yet TTBVI hit me like strong LSD with a twist of medical grade meth. Not for the faint of heart.”

Team Panaeolus

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Re: Ask Quick Questions, Get Quick Answers [Re: Letsfly24]
    #28889366 -

Do you have other germination plates? How do you make your agar and with what nutrient? What are you trying to germinate ?

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Re: Ask Quick Questions, Get Quick Answers [Re: Letsfly24] * 1
    #28889371 -

The entire plate looks like mold to me. Do you have other germination plates?

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Re: Ask Quick Questions, Get Quick Answers [Re: TheAtlantean]
    #28889372 -

Thank you! Waiting to get allowed in to the market place and I’ll step up the game. Great tips.

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Re: Ask Quick Questions, Get Quick Answers [Re: Cosmyc]
    #28889374 -

It states that it’s LB agar. I foolishly bought them on Amazon. I’ve since bought the materials to make my own. I think I’ll just trash the plate fo safety sake. No action on the other plates I did so maybe no spores came out. Always learning.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28889382 -

I got some of these: "AirPort" Lids for LC and Grain Spawn

Quote:
These reusable “airport” lids are designed to keep contaminants out of your liquid culture or grain spawn, while allowing for adequate gas exchange.  Equipped with  super heavy duty injection ports, 0.22 µm syringe filters (13 mm diameter), and rust-proof stainless steel canning bands, these lids can be re-used for years.


But then I did some research and found several posts saying that syringe filters aren't enough for grain spawn.

Will they work or do I have to return them? :bored:


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Re: Ask Quick Questions, Get Quick Answers [Re: Arctic_Fox] * 1
    #28889413 -

Arctic_Fox said:
I got some of these: "AirPort" Lids for LC and Grain Spawn

Quote:
These reusable “airport” lids are designed to keep contaminants out of your liquid culture or grain spawn, while allowing for adequate gas exchange.  Equipped with  super heavy duty injection ports, 0.22 µm syringe filters (13 mm diameter), and rust-proof stainless steel canning bands, these lids can be re-used for years.


But then I did some research and found several posts saying that syringe filters aren't enough for grain spawn.

Will they work or do I have to return them? :bored:


They ain't great for grain spawn


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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr]
    #28889428 -

cronicr said:
Arctic_Fox said:
I got some of these: "AirPort" Lids for LC and Grain Spawn

Quote:
These reusable “airport” lids are designed to keep contaminants out of your liquid culture or grain spawn, while allowing for adequate gas exchange.  Equipped with  super heavy duty injection ports, 0.22 µm syringe filters (13 mm diameter), and rust-proof stainless steel canning bands, these lids can be re-used for years.


But then I did some research and found several posts saying that syringe filters aren't enough for grain spawn.

Will they work or do I have to return them? :bored:


They ain't great for grain spawn


Yeah, the hole is really small. I'll go back to flipping the inner part of a normal lid and leaving it slightly loose.


--------------------


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Re: Ask Quick Questions, Get Quick Answers [Re: Arctic_Fox]
    #28889469 -

for those who have experience with long-term storage of plins in cryovials with sterile water: it's normal for them to turn completly black? - thought this kind of oxidation would need oxigen... starting to doubt if the cryovials are air sealed...

Edited by Psybanana (07/27/24 06:59 PM)

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Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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