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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Arctic_Fox] * 1
    #28845511 -

Where has SPG been


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Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

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Re: Ask Quick Questions, Get Quick Answers [Re: 999999999]
    #28845684 -

Gleyck said:
You're good :thumbup:
Those first jars will just colonise faster than the others, so your "minor flaw" was on the remaining jars :lol:
"How long will it be sterile for?" in theory, indefinitely... just impregnate it ASAP with spores, LC or LI


I’m keeping a jar without inoculant as a control.  Three months later it looks totally normal.


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🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
Hitchhiker's Guide to the Shroomery
Do not be concerned with the fruits of your actions, just give and do your best.
Shunryū Suzuki, Zen Mind Beginner's Mind

I start to laugh at the cosmic humor of this grumpy meat goblin scowling at everything and carrying around petty resentments like treasured turds.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28845815 -

A friend gave me a fully colonized 8 oz oat jar. What is the best way to birth it? I'm still pretty new and I've only ever done BRF pf tek before.

I was thinking put it in a shoebox with some coco coir? But not sure what the amounts/ratio should be for just a single small jar (most teks are for larger quantities, obviously). Any advice would be appreciated!

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Re: Ask Quick Questions, Get Quick Answers [Re: satanicpancake]
    #28845829 -

You could get a small container for it.
A shoebox sized tub.


--------------------

The Shroomery Manual
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Dream Interpretation
GC’s Ayahuasca Recipe
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Re: Ask Quick Questions, Get Quick Answers [Re: satanicpancake] * 1
    #28845830 -

Is it fine with some burs wheat grains after pressure cooking or do you just redo it?

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Re: Ask Quick Questions, Get Quick Answers [Re: ansr] * 1
    #28845833 -

A few burst grains is fine.


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

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Re: Ask Quick Questions, Get Quick Answers [Re: satanicpancake] * 3
    #28845847 -

satanicpancake said:
A friend gave me a fully colonized 8 oz oat jar. What is the best way to birth it? I'm still pretty new and I've only ever done BRF pf tek before.

I was thinking put it in a shoebox with some coco coir? But not sure what the amounts/ratio should be for just a single small jar (most teks are for larger quantities, obviously). Any advice would be appreciated!


You can use a Ziploc bag with some coco coir: https://www.shroomery.org/forums/showflat.php/Number/27280275

Ratio-wise, 1:2 or 1:3 (spawn:substrate) is a good place to start. You can use more substrate if you're very confident the jar is clean.


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LAGM 2026 grow log | My library/trade list

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 2
    #28846074 -

Hi team.

I've got questions around substrate shrinkage.  That's pure coir, nothing else.  Is the shrinkage not excessive?  If myc does not "eat" the non-nutritive coir, is this shrinkage then purely due to absorption of water? And, if so, was my coir too dry?  I do the "squeeze really hard, only a few drops should come out" method.  Lastly, for my second flush, should I soak this substrate after harvest, or is there nothing to do?

Kind regards, and thanks in advance.



Closeup plus metric ruler (mm) for scale:


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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA] * 1
    #28846079 -

SWBZA said:
Hi team.

I've got questions around substrate shrinkage.  That's pure coir, nothing else.  Is the shrinkage not excessive?  If myc does not "eat" the non-nutritive coir, is this shrinkage then purely due to absorption of water? And, if so, was my coir too dry?  I do the "squeeze really hard, only a few drops should come out" method.  Lastly, for my second flush, should I soak this substrate after harvest, or is there nothing to do?

Kind regards, and thanks in advance.



Closeup plus metric ruler (mm) for scale:




Yeah you should soak it after harvest. It would probably shrink less if you compress the coir as much as you can when spawning


--------------------
The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: Blueberry Muffin]
    #28846089 -

Thanks. Regarding compressing the coir - myc must breathe, yeah? Where do you find the balance between having enough air in the coir / grain mix vs. your suggestion of compressing enough to limit shrinking?

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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA]
    #28846116 -

So I had my first flush. I left the babies then misted it heavy last night. When I got up for work 5 hours later the pools of water were gone. Should I just let it ride? Should I add a new casing?

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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA]
    #28846130 -

SWBZA said:
Thanks. Regarding compressing the coir - myc must breathe, yeah? Where do you find the balance between having enough air in the coir / grain mix vs. your suggestion of compressing enough to limit shrinking?


you dont, its not an issue with coir.


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Re: Ask Quick Questions, Get Quick Answers [Re: fiddle_head] * 1
    #28846138 -

fiddle_head said:
SWBZA said:
Thanks. Regarding compressing the coir - myc must breathe, yeah? Where do you find the balance between having enough air in the coir / grain mix vs. your suggestion of compressing enough to limit shrinking?


you dont, its not an issue with coir.


Thanks

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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA] * 1
    #28846195 -

SWBZA said:
fiddle_head said:
SWBZA said:
Thanks. Regarding compressing the coir - myc must breathe, yeah? Where do you find the balance between having enough air in the coir / grain mix vs. your suggestion of compressing enough to limit shrinking?


you dont, its not an issue with coir.


Thanks


Fiddy knows what he's talking about. He compresses with fully body weight and I do too. Eliminates most side pins and myc seems to love it too.


--------------------
The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: Blueberry Muffin]
    #28846200 -

:whathesaid:

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Re: Ask Quick Questions, Get Quick Answers [Re: Blueberry Muffin]
    #28846210 -

Blueberry Muffin said:
SWBZA said:
fiddle_head said:
SWBZA said:
Thanks. Regarding compressing the coir - myc must breathe, yeah? Where do you find the balance between having enough air in the coir / grain mix vs. your suggestion of compressing enough to limit shrinking?


you dont, its not an issue with coir.


Thanks


Fiddy knows what he's talking about. He compresses with fully body weight and I do too. Eliminates most side pins and myc seems to love it too.


Damn, full body weight? I haven't put to bulk yet but going off beefs tub write up he says firmly but not with all your might.


--------------------
Where to start?

Are your plates clean?


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Re: Ask Quick Questions, Get Quick Answers [Re: boxtrotalpha] * 1
    #28846226 -




Fiddy knows what he's talking about. He compresses with fully body weight and I do too. Eliminates most side pins and myc seems to love it too.


Damn, full body weight? I haven't put to bulk yet but going off beefs tub write up he says firmly but not with all your might.


I stick an empty tub on top and put as much weight on it as I can. The better it's packed the less side pins you'll get. I have NEVER used a liner

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28846267 -

Flesh from original mushroom-> agar.
Agar-> grow.
Grow-> spore swab.
Swab-> agar.
Agar-> grow.

Is the underline grown fruit F1?

If not, please clarify why.
:takingnotes:

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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA]
    #28846311 -

SWBZA said:
Hi team.

I've got questions around substrate shrinkage.  That's pure coir, nothing else.  Is the shrinkage not excessive?  If myc does not "eat" the non-nutritive coir, is this shrinkage then purely due to absorption of water? And, if so, was my coir too dry?  I do the "squeeze really hard, only a few drops should come out" method.  Lastly, for my second flush, should I soak this substrate after harvest, or is there nothing to do?

Kind regards, and thanks in advance.



Closeup plus metric ruler (mm) for scale:





Shrinkage is a good thing and is indicative of production. Cubes do break down and consume parts of the coir. Coir is primarily comprised of complex carbohydrates such as cellulose.


--------------------
Question everything, it's very responsive.
culture storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Lithop] * 2
    #28846317 -

Lithop said:
Flesh from original mushroom-> agar.
Agar-> grow.
Grow-> spore swab.
Swab-> agar.
Agar-> grow.

Is the underline grown fruit F1?

If not, please clarify why.
:takingnotes:


no.

you start with F0, which is your starting or "wild" gen. Whichever you decide to start with, whether its selecting and stabilizing based on pheno alone or if you're making a cross. You grow out the initial f0 ms (spores) on agar and transfer until you have clean, uniform looking growth. now grow out the culture, take a clone. Grow THAT out. Now harvest spores. Now repeat this process until stable, usually 7-8 times. with each generation of inbreeding you are selecting based on pheno/desired traits both when you take a clone and when you select a fruit for your next ms, now we are on F1

its a little hard to catch on immediately if you're not very familiar with classic mendelian genetics or biology. But essentially you're going:

spores>grow>clone fruit (agar)>grow again>harvest spores

youre just alternating between growing out mss and making clones. you are narrowing the heterozygosity (wild or natural state) each time you do this selecting carefully based on your desired traits.

that represents one cycle or filial gen, which you will need to repeat until f7-f8, until stable and most if not all of the fruiting bodies are showing the desired traits.

Edited by fiddle_head (07/11/24 09:51 AM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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